Download pLX304 - TransOMIC

LOCUS
DEFINITION
Exported
9377 bp ds-DNA
circular SYN 29-JAN-2014
Lentiviral Gateway(R) destination vector with a blasticidin
resistance marker and a V5 epitope tag, for strong expression and
improved plasmid yield and viral titer.
ACCESSION
.
VERSION
.
KEYWORDS
pLX304
SOURCE
synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE
1 (bases 1 to 9377)
AUTHORS
Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja
R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver
SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C,
Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE.
TITLE
A public genome-scale lentiviral expression library of human ORFs.
JOURNAL
Nat. Methods 2011;8:659-61.
PUBMED
21706014
REFERENCE
2 (bases 1 to 9377)
AUTHORS
Root Lab
TITLE
Direct Submission
JOURNAL
Exported Monday, Mar 31, 2014 from SnapGene 2.3.2
http://www.snapgene.com
FEATURES
Location/Qualifiers
source
1..9377
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer
65..368
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter
369..572
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
protein_bind
595..719
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="attR1"
/note="recombination site for the Gateway(R) LR reaction"
promoter
744..774
/note="lac UV5 promoter"
/note="E. coli lac promoter with an ""up"" mutation"
CDS
828..1487
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS
1829..2134
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/note="ccdB"
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind
complement(2175..2299)
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="attR2"
/note="recombination site for the Gateway(R) LR reaction"
CDS
2301..2342
/codon_start=1
/product="epitope tag from simian virus 5"
/note="V5 tag"
/translation="GKPIPNPLLGLDST"
misc_feature
2343..2351
/note="stop codons"
misc_feature
2384..2972
/note="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR
3044..3277
/note="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR)
from
HIV-1"
polyA_signal
3355..3476
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin
3516..3651
/note="SV40 ori"
/note="SV40 origin of replication"
rep_origin
3858..4313
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter
4339..4443
/gene="bla"
/note="AmpR promoter"
CDS
4444..5304
/codon_start=1
/note="AmpR"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin
promoter
LTR
misc_feature
misc_feature
promoter
CDS
5475..6063
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
6530..6758
/note="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
6759..6939
/note="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
6986..7111
/note="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
7604..7837
/note="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
8241..8741
/note="hPGK promoter"
/note="human phosphoglycerate kinase 1 (PGK1) promoter"
8753..9148
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/note="BSD"
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature
9208..9325
/note="cPPT/CTS"
/note="central polypurine tract and central
sequence of HIV-1"
ORIGIN
1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg
601 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
661 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
721 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
781 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg
841 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
termination
tatatggagt
gacccccgcc
ttccattgac
gtgtatcata
cattatgccc
gtcatcgcta
tttgactcac
caccaaaatc
ggcggtaggc
atcaacaagt
attaaattag
gtcactatgg
aatgtgtgga
gagaaaaaaa
tttgaggcat
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ttcagtcagt
taaagaccgt
gcctgatgaa
gggatagtgt
tctggagtga
cgtgttacgg
tctcagccaa
acttcttcgc
tgccgctggc
ttaatgaatt
gcttactaaa
tatatactga
tacagtgaca
tccggtctgg
aaagcggaaa
tttgctgacg
gagccgttat
gatggtgatc
cccggtggtg
gccggtctcc
aaacgccatt
gtctgcaggt
ttttatgcaa
ctttcttgta
cgtagtaatg
caaaatttgt
atacgctgct
ctccttgtat
acgtggcgtg
cacctgtcag
catcgccgcc
cgtggtgttg
gattctgcgc
ttcccgcggc
gagtcggatc
gcagctgtag
tcccaacgaa
tgagcctggg
ccttgagtgc
ctcagaccct
cttattattc
tttattgcag
gcattttttt
gtctggctct
ccgcccattc
cctcggcctc
acccaattcg
tcgtgactgg
cgccagctgg
cctgaatggc
tacgcgcagc
cccttccttt
tttagggttc
tggttcacgt
tgctcaatgt
aaagaaaaat
tgctcatccg
tcacccttgt
ataccacgac
tgaaaacctg
tccctgggtg
ccccgttttc
gattcaggtt
acaacagtac
agccagataa
tatgtatacc
gttgacagcg
taagcacaac
atcaggaagg
agaacagggg
cgtctgtttg
cccctggcca
catatcgggg
gttatcgggg
aacctgatgt
cgaccatagt
aatctaattt
caaagtggtt
agctagcgct
gaaagattga
ttaatgcctt
aaatcctggt
gtgtgcactg
ctcctttccg
tgccttgccc
tcggggaaat
gggacgtcct
ctgctgccgg
tccctttggg
atcttagcca
gacaagatct
agctctctgg
ttcaagtagt
tttagtcagt
agtatttata
cttataatgg
cactgcattc
agctatcccg
tccgccccat
tgagctattc
ccctatagtg
gaaaaccctg
cgtaatagcg
gaatgggacg
gtgaccgcta
ctcgccacgt
cgatttagtg
agtgggccat
acctataacc
aagcacaagt
gaattccgta
tacaccgttt
gatttccggc
gcctatttcc
agtttcacca
accatgggca
catcatgccg
tgcgatgagt
cagtatgcgt
cgaagtatgt
acagctatca
catgcagaat
gatggctgag
ctggtgaaat
tggatgtaca
gtgcacgtct
atgaaagctg
aagaagtggc
tctggggaat
gactggatat
aatatattga
ggtaagccta
aaccggtggc
ctggtattct
tgtatcatgc
tgctgtctct
tgtttgctga
ggactttcgc
gctgctggac
catcgtcctt
tctgctacgt
ctctgcggcc
ccgcctcccc
ctttttaaaa
gctttttgct
ctaactaggg
gtgtgcccgt
gtggaaaatc
acttgcaaag
ttacaaataa
tagttgtggt
cccctaactc
ggctgactaa
cagaagtagt
agtcgtatta
gcgttaccca
aagaggcccg
cgccctgtag
cacttgccag
tcgccggctt
ctttacggca
cgccctgata
agaccgttca
tttatccggc
tggcaatgaa
tccatgagca
agtttctaca
ctaaagggtt
gttttgattt
aatattatac
tttgtgatgg
ggcagggcgg
atttgcgcgc
caaaaagagg
gttgctcaag
gaagcccgtc
gtcgcccggt
gcagtttaag
gagtgatatt
gctgtcagat
gcgcatgatg
tgatctcagc
ataaatgtca
gttgtgtttt
tatttatatc
tccctaaccc
gcgttaagtc
taactatgtt
tattgcttcc
ttatgaggag
cgcaaccccc
tttccccctc
aggggctcgg
tccttggctg
cccttcggcc
tcttccgcgt
gcgtcgactt
gaaaaggggg
tgtactgggt
aacccactgc
ctgttgtgtg
tctagcagta
aaatgaatat
agcaatagca
ttgtccaaac
cgcccatccc
ttttttttat
gaggaggctt
cgcgcgctca
acttaatcgc
caccgatcgc
cggcgcatta
cgccctagcg
tccccgtcaa
cctcgacccc
gacggttttt
gctggatatt
ctttattcac
agacggtgag
aactgaaacg
catatattcg
tattgagaat
aaacgtggcc
gcaaggcgac
cttccatgtc
ggcgtaaaga
tgatttttgc
tatgctatga
gcatatatga
gtctgcgtgc
ttattgaaat
gtttacacct
attgacacgc
aaagtctccc
accaccgata
caccgcgaaa
ggctccctta
acagtattat
attttacgtt
tctcctcggt
gacaatcaac
gctcctttta
cgtatggctt
ttgtggcccg
actggttggg
cctattgcca
ctgttgggca
ctcgcctgtg
ctcaatccag
cttcgccttc
taagaccaat
gactggaagg
ctctctggtt
ttaagcctca
actctggtaa
cgtatagtag
cagagagtga
tcacaaattt
tcatcaatgt
gcccctaact
ttatgcagag
ttttggaggc
ctggccgtcg
cttgcagcac
ccttcccaac
agcgcggcgg
cccgctcctt
gctctaaatc
aaaaaacttg
cgccctttga
acggcctttt
attcttgccc
ctggtgatat
ttttcatcgc
caagatgtgg
atgtttttcg
aatatggaca
aaggtgctga
ggcagaatgc
tctggatccg
ggtataagaa
agcagcgtat
tgtcaatatc
cgaacgctgg
gaacggctct
ataaaagaga
ccgggcgacg
gtgaacttta
tggccagtgt
atgacatcaa
tacacagcca
gtagtctgtt
tctcgttcag
ctcgattcta
ctctggatta
cgctatgtgg
tcattttctc
ttgtcaggca
gcattgccac
cggcggaact
ctgacaattc
ttgccacctg
cggaccttcc
gccctcagac
gacttacaag
gctaattcac
agaccagatc
ataaagcttg
ctagagatcc
ttcatgtcat
gaggaacttg
cacaaataaa
atcttatcat
ccgcccagtt
gccgaggccg
ctagggacgt
ttttacaacg
atcccccttt
agttgcgcag
gtgtggtggt
tcgctttctt
gggggctccc
attagggtga
cgttggagtc
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
cacgttcttt
ctattctttt
gatttaacaa
acttttcggg
atgtatccgc
agtatgagta
cctgtttttg
gcacgagtgg
cccgaagaac
tcccgtattg
ttggttgagt
ttatgcagtg
atcggaggac
cttgatcgtt
atgcctgtag
gcttcccggc
cgctcggccc
tctcgcggta
tacacgacgg
gcctcactga
gatttaaaac
atgaccaaaa
atcaaaggat
aaaccaccgc
aaggtaactg
ttaggccacc
ttaccagtgg
tagttaccgg
ttggagcgaa
acgcttcccg
gagcgcacga
cgccacctct
aaaaacgcca
atgttctttc
gctgataccg
gaagagcgcc
tggcacgaca
tagctcactc
ggaattgtga
cgcgcaatta
atgcaatact
gagagaaaaa
taggaaggca
gagatattgt
tctgagcctg
tgccttgagt
ccctcagacc
gaaagcgaaa
cacggcaaga
ctagaaggag
atgggaaaaa
atgggcaagc
aggctgtaga
tagatcatta
aatagtggac
gatttataag
aaatttaacg
gaaatgtgcg
tcatgagaca
ttcaacattt
ctcacccaga
gttacatcga
gttttccaat
acgccgggca
actcaccagt
ctgccataac
cgaaggagct
gggaaccgga
caatggcaac
aacaattaat
ttccggctgg
tcattgcagc
ggagtcaggc
ttaagcattg
ttcattttta
tcccttaacg
cttcttgaga
taccagcggt
gcttcagcag
acttcaagaa
ctgctgccag
ataaggcgca
cgacctacac
aagggagaaa
gggagcttcc
gacttgagcg
gcaacgcggc
ctgcgttatc
ctcgccgcag
caatacgcaa
ggtttcccga
attaggcacc
gcggataaca
accctcacta
cttgtagtct
gcaccgtgca
acagacgggt
atttaagtgc
ggagctctct
gcttcaagta
cttttagtca
gggaaaccag
ggcgaggggc
agagatgggt
attcggttaa
agggagctag
caaatactgg
tataatacag
tcttgttcca
ggattttgcc
cgaattttaa
cggaacccct
ataaccctga
ccgtgtcgcc
aacgctggtg
actggatctc
gatgagcact
agagcaactc
cacagaaaag
catgagtgat
aaccgctttt
gctgaatgaa
aacgttgcgc
agactggatg
ctggtttatt
actggggcca
aactatggat
gtaactgtca
atttaaaagg
tgagttttcg
tccttttttt
ggtttgtttg
agcgcagata
ctctgtagca
tggcgataag
gcggtcgggc
cgaactgaga
ggcggacagg
agggggaaac
tcgatttttg
ctttttacgg
ccctgattct
ccgaacgacc
accgcctctc
ctggaaagcg
ccaggcttta
atttcacaca
aagggaacaa
tgcaacatgg
tgccgattgg
ctgacatgga
ctagctcgat
ggctaactag
gtgtgtgccc
gtgtggaaaa
aggagctctc
ggcgactggt
gcgagagcgt
ggccaggggg
aacgattcgc
gacagctaca
tagcaaccct
aactggaaca
gatttcggcc
caaaatatta
atttgtttat
taaatgcttc
cttattccct
aaagtaaaag
aacagcggta
tttaaagttc
ggtcgccgca
catcttacgg
aacactgcgg
ttgcacaaca
gccataccaa
aaactattaa
gaggcggata
gctgataaat
gatggtaagc
gaacgaaata
gaccaagttt
atctaggtga
ttccactgag
ctgcgcgtaa
ccggatcaag
ccaaatactg
ccgcctacat
tcgtgtctta
tgaacggggg
tacctacagc
tatccggtaa
gcctggtatc
tgatgctcgt
ttcctggcct
gtggataacc
gagcgcagcg
cccgcgcgtt
ggcagtgagc
cactttatgc
ggaaacagct
aagctggagc
taacgatgag
tggaagtaag
ttggacgaac
acataaacgg
ggaacccact
gtctgttgtg
tctctagcag
tcgacgcagg
gagtacgcca
cagtattaag
aaagaaaaaa
agttaatcct
accatccctt
ctattgtgtg
acactcaacc
tattggttaa
acgcttacaa
ttttctaaat
aataatattg
tttttgcggc
atgctgaaga
agatccttga
tgctatgtgg
tacactattc
atggcatgac
ccaacttact
tgggggatca
acgacgagcg
ctggcgaact
aagttgcagg
ctggagccgg
cctcccgtat
gacagatcgc
actcatatat
agatcctttt
cgtcagaccc
tctgctgctt
agctaccaac
ttcttctagt
acctcgctct
ccgggttgga
gttcgtgcac
gtgagctatg
gcggcagggt
tttatagtcc
caggggggcg
tttgctggcc
gtattaccgc
agtcagtgag
ggccgattca
gcaacgcaat
ttccggctcg
atgaccatga
tgcaagctta
ttagcaacat
gtggtacgat
cactgaattg
gtctctctgg
gcttaagcct
tgactctggt
tggcgcccga
actcggcttg
aaaattttga
cgggggagaa
tataaattaa
ggcctgttag
cagacaggat
catcaaagga
ctatctcggt
aaaatgagct
tttaggtggc
acattcaaat
aaaaaggaag
attttgcctt
tcagttgggt
gagttttcgc
cgcggtatta
tcagaatgac
agtaagagaa
tctgacaacg
tgtaactcgc
tgacaccacg
acttactcta
accacttctg
tgagcgtggg
cgtagttatc
tgagataggt
actttagatt
tgataatctc
cgtagaaaag
gcaaacaaaa
tctttttccg
gtagccgtag
gctaatcctg
ctcaagacga
acagcccagc
agaaagcgcc
cggaacagga
tgtcgggttt
gagcctatgg
ttttgctcac
ctttgagtga
cgaggaagcg
ttaatgcagc
taatgtgagt
tatgttgtgt
ttacgccaag
atgtagtctt
gccttacaag
cgtgccttat
ccgcattgca
ttagaccaga
caataaagct
aactagagat
acagggactt
ctgaagcgcg
ctagcggagg
ttagatcgcg
aacatatagt
aaacatcaga
cagaagaact
tagagataaa
7381
7441
7501
7561
7621
7681
7741
7801
7861
7921
7981
8041
8101
8161
8221
8281
8341
8401
8461
8521
8581
8641
8701
8761
8821
8881
8941
9001
9061
9121
9181
9241
9301
9361
//
agacaccaag
acagcaagcg
gtgaattata
caaagagaag
ggttcttggg
ccagacaatt
cgcaacagca
tggctgtgga
aactcatttg
agatttggaa
taatacactc
tggaattaga
atataaaatt
tactttctat
tcccaacccc
gcggctgctc
tcttcacgtc
cgacgcttcc
cgtgacaaac
caatggcagc
ccgagagcag
ccctgttcct
tcggctccct
gcctttgtct
ccccatctct
cactggtgtc
cactgctgct
caggggcatc
gatcaaagcc
gctgccctct
gagacaaatg
tgcaggggaa
acaaattaca
gcgccggctc
gaagctttag
gccgctgatc
taaatataaa
agtggtgcag
agcagcagga
attgtctggt
tctgttgcaa
aagataccta
caccactgct
tcacacgacc
cttaattgaa
taaatgggca
attcataatg
agtgaataga
gaggggaccc
tgggcgtggt
cgttcgcagc
tgctccgccc
ggaagccgca
gcgccgaccg
cggccgggaa
gcccgcgcgg
cgttgaccga
caagaagaat
gaagactaca
aatgtatatc
gctgcggcag
ttgagcccct
atagtgaagg
ggttatgtgt
gcagtattca
agaatagtag
aaaattcaaa
gaggggg
acaagataga
ttcagacctg
gtagtaaaaa
agagaaaaaa
agcactatgg
atagtgcagc
ctcacagtct
aaggatcaac
gtgccttgga
tggatggagt
gaatcgcaaa
agtttgtgga
atagtaggag
gttaggcagg
ttgcgccttt
tccgggaaac
gtcacccgga
ctaagtcggg
cgtctcacta
cgatgggctg
ggggcggtgc
tgttccgcat
atcaccgacc
ccaccctcat
gcgtcgccag
attttactgg
ctggcaacct
gcggacggtg
acagtgatgg
gggagggcct
tccacaattt
acataatagc
attttcgggt
ggaagagcaa
gaggaggaga
ttgaaccatt
gagcagtggg
gcgcagcgtc
agcagaacaa
ggggcatcaa
agctcctggg
atgctagttg
gggacagaga
accagcaaga
attggtttaa
gcttggtagg
gatattcacc
tccaaggcag
gcagcggcgc
tcttcgccgc
aaggttcctt
gtaccctcgc
tggccaatag
gggaggcggg
tctgcaagcc
tctctcccca
tgaaagagca
cgcagctctc
gggaccttgt
gacttgtatc
ccgacaggtg
acagccgacg
gcagctgcag
taaaagaaaa
aacagacata
ttattacagg
aacaaaagta
tatgagggac
aggagtagca
aataggagct
aatgacgctg
tttgctgagg
gcagctccag
gatttggggt
gagtaataaa
aattaacaat
aaagaatgaa
cataacaaat
tttaagaata
attatcgttt
ccctgggttt
cgaccctggg
tacccttgtg
gcggttcgcg
agacggacag
cggctgctca
gtgtggggcg
tccggagcgc
gggggtacca
acggctacaa
tctagcgacg
gcagaactcg
gtcgcgatcg
cttctcgatc
gcagttggga
tagtaagaat
ggggggattg
caaactaaag
gacagcagag
agaccaccgc
aattggagaa
cccaccaagg
ttgttccttg
acggtacagg
gctattgagg
gcaagaatcc
tgctctggaa
tctctggaac
tacacaagct
caagaattat
tggctgtggt
gtttttgctg
cagacccacc
gcgcagggac
tctcgcacat
ggccccccgg
gcgtgccgga
cgccagggag
gcagggcgcg
gtagtgtggg
acgtcggcag
ccatggccaa
tcaacagcat
gccgcatctt
tggtgctggg
gaaatgagaa
tgcatcctgg
ttcgtgaatt
tctagatctt
gggggtacag
aattacaaaa
atccactttg