Download Heterogeneous HER2 antigen expression among individual

Heterogeneous
HER2 antigen expression
among individual
Circulating Tumor Cells
within
breast cancer patients
Julie Smith, DVM, PHD
Postdoc / lecturer @ Biomedical Laboratory Science,
Department of Technology
DET SUNDHEDSFAGLIGE OG TEKNOLOGISKE FAKULTET
Just so you know…. Agenda!
Background:
- HER2 expression in relation to breast cancer?
- Primary tumors vs metastases – How do we treat?
- What are CTCs? A liquid Biopsy? CytoTrack
Materials and Methods:
- Development of HER2 characterization on Tumor Cell Lines
- Testing HER2 characterisation on Breast Cancer Patients
Results:
- The HER2 ICC method
- HER2 on CTCs of 7 breast cancer patients
Conclusion and round off
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BACKGROUND
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BACKGROUND
HER2 neg
HER2 pos
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BACKGROUND
?
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CTC = circulating tumour cells
EMT = epithelial-to-mesenchymal transition
MET = mesenchymal-to-epithelial transition
DTC = disseminated tumour cells
BACKGROUND
Liquid Biopsy?
Scanning
Imaging
HER2
ICC/FISH
ICC = ImmuneCytoChemistry
staining
Revisting
Reimaging
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MATERIALS AND METHODS
To develop: HER2-staining protocol for CytoDisc:
Tumor Cell Lines spiked in healthy donor blood
Mix of known HER2 negative and positive (SKBR-3, CALU-3, MB231, MCF7)
To test: the developed HER2 staining protocol in vivo
Breast Cancer Patients, n = 11
from The Maria Sklodowska-Curie Memorial Cancer Centre and Institute of
Oncology, Warsaw, Poland.
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RESULTS
Tumor cell line studies:
Incubate CytoDisc with CTCs in:
•
1:50 Anti-HER2 diluted in PBS buffer
•
For 1 hour
•
In darkness
•
At room temperature
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RESULTS
Breast cancer in vivo studies:
CTCs were detected in 7 of 11 patients ranging from 1 to 189 CTC.
Table 1. CTC Enumeration and Characterization
Patient
CTC total
(n)
HER2+
(n)
HER2+
(%)
A
189
0
0%
B
1
0
0%
C
0
0
-
D
5
0
0%
E
0
0
-
F
0
0
-
G
14
12
86%
H
2
2
100%
I
22
7
32%
J
4
4
100%
K
0
0
-
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CONCLUSION
A novel method for CTC enumeration and HER2 characterization is described.
CTC heterogeneity was observed in breast cancer patients.
Future clinical studies are on-going to examine the clinical significance of
these findings.
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THANK YOU
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ROUND OFF
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