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Table 3: Antibodies Species Goat Rabbit Antigen USPL1 PRPF6 PRPF4 Coilin UBF GFP snRNP200 EF-TUD ICE1 ELL Mouse Lamin B1 RNAP II (N20) SMN Y12 Fibrillarin 72B9 GFP GAPDH ASF/SF2 clone 103 Coilin a) clone 5P10 b) clone IH10 PRPF8 (E5) FLAG (M2) ELL tubulin (DM1A) RNAPII CTD 4H8 Chicken PML Company/reference Home-made Abcam HPA/Sigma Aldrich Proteintech (IF Fig. 1A/C/D; S1B; 4C, 7B-C) HPA/Sigma Aldrich Cell signaling (Fig. 5C) HPA/Sigma Aldrich HPA/Sigma Aldrich Gift from A. Shilatifard, Stowers Institute, Kansas City, US Gift from A. Shilatifard, Stowers Institute, Kansas City, US (IF) abcam Santa Cruz IF: Immunoglobe/ WB: BD (Fig. 4)/gift from O. Gruss, ZMBH, Germany (Fig. S1A)* DCP, Dundee, UK (Fig. 4); Abcam (Fig. S1B) Cytoskeleton Roche (Fig. 4B) Abcam Invitrogen a) IF: Monoclonal supernatant (Fig. 1B, 2A, 5C, 6C, 7A, S3B) b) WB: Abcam (Fig. 4)/Santa Cruz (Fig. S1A) Santa Cruz Sigma Aldrich Bethyl (WB) Sigma abcam Gift from R. Hay, University of Dundee Comment (Schulz et al., 2012) (Smith et al., 2011) *(Meister et al., 2001) (Pettersson et al., 1984) (Geoffroy et al., 2010) Supplemental References Geoffroy, M.C., E.G. Jaffray, K.J. Walker, and R.T. Hay. 2010. Arsenic-induced SUMO-dependent recruitment of RNF4 into PML nuclear bodies. Molecular biology of the cell. 21:4227-4239. Meister, G., D. Buhler, R. Pillai, F. Lottspeich, and U. Fischer. 2001. A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs. Nature cell biology. 3:945-949. Pettersson, I., M. Hinterberger, T. Mimori, E. Gottlieb, and J.A. Steitz. 1984. The structure of mammalian small nuclear ribonucleoproteins. Identification of multiple protein components reactive with anti-(U1)ribonucleoprotein and anti-Sm autoantibodies. The Journal of biological chemistry. 259:5907-5914. Schulz, S., G. Chachami, L. Kozaczkiewicz, U. Winter, N. Stankovic-Valentin, P. Haas, K. Hofmann, H. Urlaub, H. Ovaa, J. Wittbrodt, E. Meulmeester, and F. Melchior. 2012. Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions. EMBO reports. 13:930-938. Smith, E.R., C. Lin, A.S. Garrett, J. Thornton, N. Mohaghegh, D. Hu, J. Jackson, A. Saraf, S.K. Swanson, C. Seidel, L. Florens, M.P. Washburn, J.C. Eissenberg, and A. Shilatifard. 2011. The little elongation complex regulates small nuclear RNA transcription. Molecular cell. 44:954-965.