Download Schulz, S., G. Chachami, L. Kozaczkiewicz, U. Winter, N. Stankovic

Table 3: Antibodies
Species
Goat
Rabbit
Antigen
USPL1
PRPF6
PRPF4
Coilin
UBF
GFP
snRNP200
EF-TUD
ICE1
ELL
Mouse
Lamin B1
RNAP II (N20)
SMN
Y12
Fibrillarin 72B9
GFP
GAPDH
ASF/SF2 clone 103
Coilin
a) clone 5P10
b) clone IH10
PRPF8 (E5)
FLAG (M2)
ELL
 tubulin (DM1A)
RNAPII CTD 4H8
Chicken PML
Company/reference
Home-made
Abcam
HPA/Sigma Aldrich
Proteintech (IF Fig. 1A/C/D; S1B; 4C, 7B-C)
HPA/Sigma Aldrich
Cell signaling (Fig. 5C)
HPA/Sigma Aldrich
HPA/Sigma Aldrich
Gift from A. Shilatifard, Stowers Institute,
Kansas City, US
Gift from A. Shilatifard, Stowers Institute,
Kansas City, US (IF)
abcam
Santa Cruz
IF: Immunoglobe/ WB: BD (Fig. 4)/gift from
O. Gruss, ZMBH, Germany (Fig. S1A)*
DCP, Dundee, UK (Fig. 4); Abcam (Fig. S1B)
Cytoskeleton
Roche (Fig. 4B)
Abcam
Invitrogen
a) IF: Monoclonal supernatant (Fig. 1B, 2A,
5C, 6C, 7A, S3B)
b) WB: Abcam (Fig. 4)/Santa Cruz (Fig. S1A)
Santa Cruz
Sigma Aldrich
Bethyl (WB)
Sigma
abcam
Gift from R. Hay, University of Dundee
Comment
(Schulz et al., 2012)
(Smith et al., 2011)
*(Meister et al.,
2001)
(Pettersson et al.,
1984)
(Geoffroy et al.,
2010)
Supplemental References
Geoffroy, M.C., E.G. Jaffray, K.J. Walker, and R.T. Hay. 2010. Arsenic-induced SUMO-dependent
recruitment of RNF4 into PML nuclear bodies. Molecular biology of the cell. 21:4227-4239.
Meister, G., D. Buhler, R. Pillai, F. Lottspeich, and U. Fischer. 2001. A multiprotein complex mediates
the ATP-dependent assembly of spliceosomal U snRNPs. Nature cell biology. 3:945-949.
Pettersson, I., M. Hinterberger, T. Mimori, E. Gottlieb, and J.A. Steitz. 1984. The structure of
mammalian small nuclear ribonucleoproteins. Identification of multiple protein components
reactive with anti-(U1)ribonucleoprotein and anti-Sm autoantibodies. The Journal of
biological chemistry. 259:5907-5914.
Schulz, S., G. Chachami, L. Kozaczkiewicz, U. Winter, N. Stankovic-Valentin, P. Haas, K. Hofmann, H.
Urlaub, H. Ovaa, J. Wittbrodt, E. Meulmeester, and F. Melchior. 2012. Ubiquitin-specific
protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions. EMBO
reports. 13:930-938.
Smith, E.R., C. Lin, A.S. Garrett, J. Thornton, N. Mohaghegh, D. Hu, J. Jackson, A. Saraf, S.K. Swanson,
C. Seidel, L. Florens, M.P. Washburn, J.C. Eissenberg, and A. Shilatifard. 2011. The little
elongation complex regulates small nuclear RNA transcription. Molecular cell. 44:954-965.