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RADIATION INDUCED CHANGES IN LEVELS OF SELECTED PROTEINS IN PERIPHERAL BLOOD SERUM OF BREAST CANCER PATIENTS AS A POTENTIAL TRIAGE BIODOSIMETER FOR LARGE-SCALE RADIOLOGICAL EMERGENCIES Deperas-Kaminska Marta1,2, Bajinskis Ainars1,3, Marczyk Michal4, Polanska Joanna4, Wersäll Peter5, LidbrinkElisabet5, AinsburyElizabeth A.6, Guipaud Oliver7, Benderitter Marc7, Haghdoost Siamak1 and Wojcik Andrzej1 1: CRPR, Stockholm University, Sweden; 2: Institute of Mother and Child, Warszawa, Poland; 3: Faculty of Medicine, University of Latvia; 4: Data Mining Group, Institute of Automatic Control, Silesian University of Technology, Gliwice, Poland; 5: Division of Radiotherapy, Radiumhemmet, Karolinska University Hospital, Stockholm, Sweden; 6: Public Health England Centre for Radiation, Chemical and Environmental Hazards, Chilton, Didcot, Oxford; 7: Institute for Radiological Protection and Nuclear Safety, France. Corresponding author’s email: [email protected] The threat of a large scale radiological emergency, where thousands of people may require fast biological dosimetry for the purpose of triage, make it necessary to search for new, high throughput biological dosimeters. We tested an assay based on the quantitative analysis of selected proteins in peripheral blood serum. We were particularly interested in testing proteins that are specific to irradiation of skin, as these can be used in cases of partial body exposure. Candidate proteins were identified in an earlier study with mice, where skin of the animals was exposed to different doses of radiation and global expression of serum proteins was analysed. Eight proteins were found, the expression of which showed a consistent doseresponse relationship. Human analogues of these proteins were identified and their expression was measured in peripheral blood serum of 16 breast cancer patients undergoing external beam radiotherapy. The proteins were Apolipoprotein E; Apolipoprotein H; Complement protein 7; Prothrombinase; Pantothenate Kinase 4; Alpha-2-macroglobulin; Fetuin B and Alpha-1-Anti-Chymotrypsin. Measurements were carried out in blood samples collected prior to exposure (control), on the day after 1 fraction (2 Gy), on the day after 5 fractions (10 Gy), on the day after 10 fractions (20 Gy) and 1 month after 23-25 fractions (total dose of 46-50 Gy). Multivariate analysis was carried out and a multinomial logistic regression model was built. The results indicate that the combined analysis of Apolipoprotein E, Factor X and Pantothenate Kinase 4 allows discriminating between exposure to 2 Gy and lower and between 10 Gy and higher. The discrimination is possible up to one month after exposure. Funded by European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement number 241536.