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EVALUATION OF PROTEINURIA IN CATS: COMPARISON BETWEEN COOMASSIE BLUE AND PYROGALLOL REDMOLYBDATE M. Giraldia, S. Patrinieria, G. Rossia, W. Bertazzolob, P. Scarpaa 1 Department of Veterinary Sciences and Public Health, University of Milan, Via Celoria 10, Milano 20133, Italy;2Veterinary Animal Hospital Città di Pavia, Pavia, Italy Pyrogallol Red-Molybdate (PRM) and CoomassieBlu (CB) are the commonest assays used for evaluation of urinary protein (UP) concentration. Data about method-dependent differences are lacking. Therefore, the aims of this study were to compare UPs and urinary protein:creatinine (UPC) ratios recorded with PRM and CB and to evaluate intra-assay coefficients of variations (CV). Urine samples were collected from 58 client-owned cats by ultrasonographically-guided cystocentesis irrespective of results of sediment or of underlining diseases. UPs were measured with PRM and CB in triplicate. The mean values were used to calculate UPC ratios. The Wilcoxon signed rank test was performed to investigate the differences between UP obtained with PRR and CB and between calculated UPC ratios. Correlation between methods was assessed with the Spearman test and agreement with Passing-Bablok and Bland-Altman tests.CV were calculated in 15 samples by 20 repeated measurements of UPs (with both methods). Concordance between UPC ratios of both methods in classifying patients as proteinuric, borderline proteinuric or non proteinuric(according to IRIS sub-staging) was assessed using Cohen’s k coefficient test. Proteinuria and UPC ratio showed statistically differences between methods (p<0,0001) with CB method having higher values. Agreement between methods showed constant and proportional errors. The two methods were correlated for both UP and UPC (p<0,0001).All intra-assay CVs were <10%. Concordance in classifying samples was moderate (k=0,476). CB method was precise at any level of proteinuria but the higher UPC obtained with CB compared to PRM may affect interpretation and clinical decisions according to the IRIS guidelines.