Download Detection of E. coli Bacteria by Diffractive Optics (Application Brief)

APPLICATION BRIEF
3EROLOGICAL4ESTINGs0ATHOGEN$ETECTIONs&OOD4ESTING
Detection of E. coli Bacteria by Diffractive Optics
4HEABILITYTORAPIDLYDETECTBACTERIAINAVARIETYOFCRUDEBIOLOGICALSAMPLESCANSIGNIFICANTLYACCELERATETHEDIAGNOSISOFBACTERIALINFECTIONSASWELLASTHEIDENTIFICATIONOFBACTERIALCONTAMINATIONINFOODANDENVIRONMENTAL
SAMPLES#URRENTMETHODSFORDETECTIONAREOFTENLABORINTENSIVEEXPENSIVEANDREQUIRESPECIALIZEDPERSONNELTO
PERFORMMAKINGTHEMUNSUITABLEASRAPIDONSITEDETECTIONTOOLS(OWEVERGIVENTHELARGESIZEOFBACTERIATHEY
AREWELLSUITEDFORDETECTIONUSINGDIFFRACTIVEOPTICSEVENWHENPRESENTINLOWTITERS)NTHEEXAMPLEBELOWE. coli
0157WASDETECTEDINSPINACHEXTRACTSBYREALTIMEDIFFRACTIVEOPTICSUSINGANAVIDINCOATEDSENSOR
$IFFRACTIVE)NTENSITY$)
10
E coli in
spinach
extract
8
TMB
Order of addition:
Biotinylated rabbit anti-goat
antibody
6
Polyclonal goat antibody against
E. coli O157 (Capture Antibody)
4
Detection antibody against
E. coli O157
2
!NTIBODYCONJUGATEDTO
HORSERADISHPEROXIDASE(20
0
0
2000
4000
6000
8000
TMB /XIDATIONANDPRECIPITATIONOF
TETRAMETHYLBENZIDINE4-"
4IMES
Highlights:
s2APIDUNDERMINUTESANDEASYTOUSEDETECTIONPLATFORMFORBACTERIAINBIOLOGICALSAMPLES
s#OMPATIBLEWITHAWIDEVARIETYOFCRUDEBIOLOGICALSAMPLESINCLUDINGBODILYFLUIDSANDFOODEXTRACTS
s.OSAMPLEPREPROCESSINGORPRECULTUREREQUIREDUNLIKETRADITIONALIMMUNOASSAYSOR0#2TECHNIQUES
FORPATHOGENDETECTION
s#OMPATIBLEWITHAVARIETYOFASSAYFORMATSANDAMPLIFICATIONSTRATEGIESEG4-"OXIDATIONANDPRECIPITATION
ASSHOWNABOVEFORHIGHERSENSITIVITY
s#ANBEMULTIPLEXEDUSING!XELASPANEL0LUSÛ3ENSORSFORMULTIPLEPATHOGENSCREENINGASSAYS
s0OTENTIALAPPLICATIONSFORRAPIDINFECTIOUSDISEASETESTINGANDFOODENVIRONMENTALTESTING
www.axela.com
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[email protected]
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