Download Answer is C Explanation: During the PCR process, specific primers

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project

Document related concepts

Hospital-acquired infection wikipedia , lookup

Urinary tract infection wikipedia , lookup

DNA vaccination wikipedia , lookup

Transcript
Answer is C
Explanation: During the PCR process, specific primers are DNA oligomers that define the ends of the
target sequences to be amplified. Each round of the amplification process begins with denaturation at
high temperature which denatures, or separates, the double-stranded DNA into complementary singlestranded DNA. The annealing step occurs when the temperature is reduced to that which is appropriate
for the primers to pair up and anneal at specific sequences on the long, single-stranded DNA; this short
stretch of double-stranded DNA provides a starting point for the polymerase to replicate the desired
sequence. (It would be possible for the original complementary DNAs to again pair and anneal at this
temperature, but the relatively high concentration of primers ensures that they are the most likely
annealing to take place.) The extension step takes place at the ideal temperature for the polymerase to
recognize the primer-annealed stretch of double-stranded DNA and produce a complementary strand
along the target sequence. Thus, the correct statement is “extension allows the target sequence to be
replicated by the polymerase.”
Resource: https://www-clinicalkey-com.proxy1.cl.msu.edu/#!/content/book/3-s2.0B978032329568000067X
Answer is B
Explanation: Patients with diabetes are particularly prone to UTIs, and this patient has had them before,
so clinical suspicion must always be high even with minimal symptoms. In this case, however, the
urinalysis findings strongly suggest that she is urinating frequently because her glucose is running high,
resulting in glucose in the urine, which often results in frequent urination (typically increased amounts
of urine, termed polyuria). With all four of these componets of the urinalysis being normal however
(nitrite, luek. esterase, WBC and bacteria), the post-test probability is extremely low and the diagnosis is
essentially excluded.
Resource: Primary Care Medicine: Office Evaluation and Management of the Adult Patient, 7e › Chapter
133: Approach to Dysuria and Urinary Tract Infections in Women
Answer is C
Explanation:
Excerpt from MDHHS Expedited Partner Therapy (EPT) For Chlamydia and Gonorrhea: The partners of
infected clients within the 60 days prior to treatment are the best candidates for EPT as they are at
highest risk for infection. If the last sexual encounter was more than 60 days prior, the most recent
sexual partner should be treated. There is no limit on how many partners can be provided treatment via
EPT. A combination of partner strategies can also be used. For example, a patient with several partners
may refer one or more partners to the clinic and take EPT for other partners. If a partner is pregnant,
every effort should be made to contact her for a referral to pregnancy services and/or pre-natal care.
Resource: http://www.michigan.gov/mdhhs/0,5885,7-339-71550_5104_31274-12538-,00.html#CD%20Reporting