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Answer is C Explanation: During the PCR process, specific primers are DNA oligomers that define the ends of the target sequences to be amplified. Each round of the amplification process begins with denaturation at high temperature which denatures, or separates, the double-stranded DNA into complementary singlestranded DNA. The annealing step occurs when the temperature is reduced to that which is appropriate for the primers to pair up and anneal at specific sequences on the long, single-stranded DNA; this short stretch of double-stranded DNA provides a starting point for the polymerase to replicate the desired sequence. (It would be possible for the original complementary DNAs to again pair and anneal at this temperature, but the relatively high concentration of primers ensures that they are the most likely annealing to take place.) The extension step takes place at the ideal temperature for the polymerase to recognize the primer-annealed stretch of double-stranded DNA and produce a complementary strand along the target sequence. Thus, the correct statement is “extension allows the target sequence to be replicated by the polymerase.” Resource: https://www-clinicalkey-com.proxy1.cl.msu.edu/#!/content/book/3-s2.0B978032329568000067X Answer is B Explanation: Patients with diabetes are particularly prone to UTIs, and this patient has had them before, so clinical suspicion must always be high even with minimal symptoms. In this case, however, the urinalysis findings strongly suggest that she is urinating frequently because her glucose is running high, resulting in glucose in the urine, which often results in frequent urination (typically increased amounts of urine, termed polyuria). With all four of these componets of the urinalysis being normal however (nitrite, luek. esterase, WBC and bacteria), the post-test probability is extremely low and the diagnosis is essentially excluded. Resource: Primary Care Medicine: Office Evaluation and Management of the Adult Patient, 7e › Chapter 133: Approach to Dysuria and Urinary Tract Infections in Women Answer is C Explanation: Excerpt from MDHHS Expedited Partner Therapy (EPT) For Chlamydia and Gonorrhea: The partners of infected clients within the 60 days prior to treatment are the best candidates for EPT as they are at highest risk for infection. If the last sexual encounter was more than 60 days prior, the most recent sexual partner should be treated. There is no limit on how many partners can be provided treatment via EPT. A combination of partner strategies can also be used. For example, a patient with several partners may refer one or more partners to the clinic and take EPT for other partners. If a partner is pregnant, every effort should be made to contact her for a referral to pregnancy services and/or pre-natal care. Resource: http://www.michigan.gov/mdhhs/0,5885,7-339-71550_5104_31274-12538-,00.html#CD%20Reporting