Download File - Aqua Pro Team

When the human body is infected with a pathogen, the immune system responds by making antibodies.
These antibodies bind to the antigen produced by the pathogen. The antibody attached to the antigen is
called an antigen-antibody complex. The antigen-antibody complex is then either destroyed or removed
from the body. An enzyme linked immunoabsorbant assay or ELISA test is used to determine if a patient
is infected with a pathogen by testing said patient for antibodies. An ELISA works by first adding an
antigen to the wells of a plastic well. This antigen will bind to the plastic. The wells are then rinsed to
remove any antigen not attached to the plastic. Then a secondary antibody is added to the well. A
secondary antibody is an antibody from an animal other than a human. This antibody is linked with an
enzyme. The secondary antibody will then bind to the primary antibody. The well is then rinsed out again
to remove free-floating secondary antibody. The last step is to add a pigment that reacts with the enzyme
that is liked to the secondary antibody. The continued presence of the secondary antibody depends on
the existence of a primary antibody. If the secondary antibody (and therefore the primary antibody) is
present, the well will turn blue. If the secondary antibody is absent, the well will not change color.
In this particular variation of the "Indirect ELISA test" the antigen was bound to a circular cellulose filter
paper. Next, a sample of the suspected contaminated water was added to the well (B). Given the
presence of human or animal E. Histolytica antibody, binding would occur between the antibody and the
antigen. Furthermore, the enzyme linked secondary antibody was added and allowed to bind to the
primary antibody obtained from the contaminated water source (C). Any of the unbound antibody was
washed with an aqueous buffer solution. Finally the enzyme substrate was added and given the presence
of bound antibody (from the feces in the water source) a color change was noted.
This product embraces responsible design since it only uses very small amount of harmless chemicals
and biochemical products. The cell's sturdy design insures that the contaminated water samples and
chemicals are responsibly disposed insuring that neither the chemicals nor the contaminated water return
into the ecosystem. Finally, our forthcoming online data tracking allows the effective communication of the
tests data available to people all over the world regardless of location.