Download for Total Vitamin B1 (Thiamin) - Official Methods of Analysis of AOAC

AOAC SMPR 2015.002
Table 1. Method performance requirementsa
Analytical range
Standard Method Performance RequirementsSM
(SMPRs) for Total Vitamin B1 (Thiamin) in Infant and
Adult/ Pediatric Nutritional Formula
Intended Use: Reference Method for Dispute Resolution
1 Applicability
Determinations of total vitamin B1 (thiamin) in all forms
of infant, and adult, and/or pediatric formulas (powders,
ready-to-feed liquids, and liquid concentrates). Total B1 defined as
the sum of thiamin (CAS No. 70-16-6), thiamin phosphate (CAS
No. 532-40-1), thiamin diphosphate (CAS No. 154-87-0), and
thiamin triphosphate (CAS No. 3475-65-8). All data should be
mass corrected and expressed as thiamin ion.
20–2000b
Limit of quantitation (LOQ)
≤20b
Recovery
90–110%
Repeatability (RSDr)
≤5%
Reproducibility (RSDR)
≤10%
a
Concentrations apply to: (a) “ready-to-feed” liquids “as is”;
(b) reconstituted powders (25 g into 200 g water); and (c) liquid
concentrates diluted 1:1 by weight.
b
μg /100 g reconstituted final product.
Any analytical technique that meets the following method
performance requirements is acceptable.
repeatability standard deviation (SDr); or % repeatability relative
standard deviation (%RSDr).
Reproducibility.—The standard deviation or relative standard
deviation calculated from among-laboratory data. Expressed
as the reproducibility relative standard deviation (SDR); or %
reproducibility relative standard deviation (%RSDR).
Vitamin B1 (thiamin).—2-[3-[(4-Amino-2-methyl-pyrimidin5-yl)methyl]-4-methyl-thiazol-5-yl] ethanol. CAS No. 70-16-6
(including all forms listed in applicability statement above).
3 Definitions
4 Method Performance Requirements
2 Analytical Technique
Accuracy (corresponds to the VIM definition for “trueness”).—
The closeness of agreement between the average of an infinite
number of replicate measured quantity values and a reference
quantity value.
Adult/pediatric formula.—Nutritionally complete, specially
formulated food, consumed in liquid form, which may constitute
the sole source of nourishment, made from any combination of
milk, soy, rice, whey, hydrolyzed protein, starch, and amino acids,
with and without intact protein.
Infant formula.—Breast-milk substitute specially manufactured
to satisfy, by itself, the nutritional requirements of infants during
the first months of life up to the introduction of appropriate
complementary feeding (Codex Standard 72-1981), made from any
combination of milk, soy, rice, whey, hydrolyzed protein, starch,
and amino acids, with and without intact protein.
Limit of detection (LOD).—The minimum concentration or mass
of analyte that can be detected in a given matrix with no greater
than 5% false-positive risk and 5% false-negative risk.
Limit of quantitation (LOQ).—The minimum concentration
or mass of analyte in a given matrix that can be reported as a
quantitative result
Repeatability.—Variation arising when all efforts are made
to keep conditions constant by using the same instrument and
operator, and repeating during a short time period. Expressed as the
See Table 1.
5 System Suitability Tests and/or Analytical Quality Control
Suitable methods will include blank check samples, and check
standards at the lowest point and midrange point of the analytical
range.
6 Reference Material(s)
NIST Standard Reference Material® (SRM) 1849a Infant/Adult
Nutritional Formula or equivalent. The SRM is a milk-based, hybrid
infant/adult nutritional powder prepared by a manufacturer of
infant formula and adult nutritional products. A unit of SRM 1849
consists of 10 packets, each containing approximately 10 g of
material. Certified value of NIST 1849a is 12.57 ± 0.98 mg/kg
vitamin B1 (thiamin).
7 Validation Guidance
Recommended level of validation: Official Methods of
AnalysisSM.
8 Maximum Time-to-Result
No maximum time.
Approved by AOAC Stakeholder Panel on Infant Formula and
Adult Nutritionals (SPIFAN). Final Version Date: April 17, 2015.
Effective Date: April 17, 2015.
© 2015 AOAC INTERNATIONAL