Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Correlation of human T-cell lymphotropic virus type 1 (HTLV-1) mRNA with proviral DNA load, virus-specific CD8+ T cells, and disease severity in HTLV-1–associated myelopathy (HAM/TSP) by Yoshihisa Yamano, Masahiro Nagai, Meghan Brennan, Carlos A. Mora, Samantha S Soldan, Utano Tomaru, Norihiro Takenouchi, Shuji Izumo, Mitsuhiro Osame, and Steven Jacobson Blood Volume 99(1):88-94 January 1, 2002 ©2002 by American Society of Hematology Validation of real-time quantitative RT-PCR assay.(A) To determine interassay CV% for this realtime RT-PCR assay, cDNA from the HTLV-1–infected MT-2 cell line was serially diluted with cDNA from the uninfected Jurkat cell line and HTLV-1 tax mRNA load was ... Yoshihisa Yamano et al. Blood 2002;99:88-94 ©2002 by American Society of Hematology Sensitivity and specificity of real-time RT-PCR assay.(A) To determine the sensitivity of this realtime RT-PCR assay, HTLV-1 tax mRNA load was determined from MT-2 cells serially diluted with PBMCs from an HTLV-1− healthy donor. Yoshihisa Yamano et al. Blood 2002;99:88-94 ©2002 by American Society of Hematology HTLV-I tax mRNA load in HAM/TSP patients and ACs. HTLV-1 tax mRNA load was assessed in PBMCs from 16 HAM/TSP patients (HAM) and 8 asymptomatic HTLV-I carriers (AC). Yoshihisa Yamano et al. Blood 2002;99:88-94 ©2002 by American Society of Hematology Correlation between HTLV-1 proviral DNA load and HTLV-1 tax mRNA load in HAM/TSP patients. Yoshihisa Yamano et al. Blood 2002;99:88-94 ©2002 by American Society of Hematology Correlation between HTLV-1 tax mRNA load and HTLV-1 Tax protein expression in vitro. Yoshihisa Yamano et al. Blood 2002;99:88-94 ©2002 by American Society of Hematology Correlation of HTLV-1 Tax-specific CD8+ T-cell frequency with HTLV-1 tax mRNA load.The frequency of HTLV-1 Tax 11-19/HLA-A*0201-specific CD8+ T cells (expressed as a percentage of total CD8+ T cells) was analyzed using a PE-conjugated HLA-A*0201 tetramer fo... Yoshihisa Yamano et al. Blood 2002;99:88-94 ©2002 by American Society of Hematology