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Correlation of human T-cell lymphotropic virus type 1
(HTLV-1) mRNA with proviral DNA load, virus-specific CD8+
T cells, and disease severity in HTLV-1–associated
myelopathy (HAM/TSP)
by Yoshihisa Yamano, Masahiro Nagai, Meghan Brennan, Carlos A. Mora, Samantha
S Soldan, Utano Tomaru, Norihiro Takenouchi, Shuji Izumo, Mitsuhiro Osame, and
Steven Jacobson
Blood
Volume 99(1):88-94
January 1, 2002
©2002 by American Society of Hematology
Validation of real-time quantitative RT-PCR assay.(A) To determine interassay CV% for this realtime RT-PCR assay, cDNA from the HTLV-1–infected MT-2 cell line was serially diluted with cDNA
from the uninfected Jurkat cell line and HTLV-1 tax mRNA load was ...
Yoshihisa Yamano et al. Blood 2002;99:88-94
©2002 by American Society of Hematology
Sensitivity and specificity of real-time RT-PCR assay.(A) To determine the sensitivity of this realtime RT-PCR assay, HTLV-1 tax mRNA load was determined from MT-2 cells serially diluted with
PBMCs from an HTLV-1− healthy donor.
Yoshihisa Yamano et al. Blood 2002;99:88-94
©2002 by American Society of Hematology
HTLV-I tax mRNA load in HAM/TSP patients and ACs. HTLV-1 tax mRNA load was assessed in
PBMCs from 16 HAM/TSP patients (HAM) and 8 asymptomatic HTLV-I carriers (AC).
Yoshihisa Yamano et al. Blood 2002;99:88-94
©2002 by American Society of Hematology
Correlation between HTLV-1 proviral DNA load and HTLV-1 tax mRNA load in HAM/TSP patients.
Yoshihisa Yamano et al. Blood 2002;99:88-94
©2002 by American Society of Hematology
Correlation between HTLV-1 tax mRNA load and HTLV-1 Tax protein expression in vitro.
Yoshihisa Yamano et al. Blood 2002;99:88-94
©2002 by American Society of Hematology
Correlation of HTLV-1 Tax-specific CD8+ T-cell frequency with HTLV-1 tax mRNA load.The
frequency of HTLV-1 Tax 11-19/HLA-A*0201-specific CD8+ T cells (expressed as a percentage of
total CD8+ T cells) was analyzed using a PE-conjugated HLA-A*0201 tetramer fo...
Yoshihisa Yamano et al. Blood 2002;99:88-94
©2002 by American Society of Hematology
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