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Anti-MHC Class I Monomorphic monoclonal antibody, clone DWT33 [FITC] Cat.No: DCABY-172 Lot. No. (See product label) PRODUCT INFORMATION Specificity Mouse anti Horse MHC Class I Monomorphic antibody, clone DWT33 recognizes monomorphic equine MHC Class I and was classified at the International Equine Leucocyte Antigen Workshop. Immunogen Equine leucocytes Isotype IgG2a Source/Host Mouse Species Reactivity Horse Clone DWT33 Conjugate FITC Applications Flow Cytometry Preparation Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Procedure Conjugated Antibodies Format Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid Size 100 μg Buffer Phosphate buffered saline. Storage Store at +4°C for one month or at -20°C for longer.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Background Introduction MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen. Keywords A 28; A 9; Antigen presenting molecule; Aw 24; Aw 68; CLASS I HISTOCOMPATIBILITY ANTIGEN; H2 K1; H2K; HLA A; HLA class I histocompatibility antigen A 1 alpha chain; HLA class I histocompatibility antigen A 11 alpha chain; HLA class I histocompatibility antigen A 2 alpha chain; HLA class I histocompatibility antigen A 24 alpha chain; HLA class I histocompatibility antigen A 3 alpha chain; HLA class I histocompatibility antigen A 30 alpha chain; HLA class I histocompatibility antigen A 32 alpha chain; HLA class I histocompatibility antigen A 68 alpha chain; HLAA; Leukocyte antigen class I A; Major histocompatibility complex class I A; MHC class I antigen A 1; MHC class I antigen A*11; MHC class I antigen A*2; MHC class I antigen A*24; MHC class I antigen A*3; MHC class I antigen A*30; MHC class I antigen A*32; MHC class I antigen A*68; MHC class I antigen HLA A heavy chain; MHC class I heavy chain H2 K Antigen Gene Information Creative Diagnostics. All rights reserved 45-1 Ramsey Road Shirley, NY 11967, USA Tel: 1-631-624-4882 Fax: 1-631-938-8221 E-mail: [email protected] www.creative-diagnostics.com 1 References 1. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 62:101-143. 2. Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity. Vet Immunol Immunopathol. 131:1-8. Creative Diagnostics. All rights reserved 45-1 Ramsey Road Shirley, NY 11967, USA Tel: 1-631-624-4882 Fax: 1-631-938-8221 E-mail: [email protected] www.creative-diagnostics.com 2