Download Integrated optoelectronics and nanomechanical biosensors for

Nanobiosensors based on optoelectronics and nanomechanical
transducers for genomics and proteomics applications
L.M. Lechuga, J. Tamayo, M. Calleja, A. Calle and C. Domínguez
Grupo de Biosensores, Centro Nacional de Microeléctronica (CNM), CSIC.
Isaac Newton, 8 – 28760 Tres Cantos, Madrid (Spain) [email protected]
The Biosensor Group (National Microelectronic Center, CSIC, Spain) is focus on the
development of integrated sensors and biosensors based on evanescent wave sensing
and on nanomechanical changes, with high sensitivity and selectivity for application in
the functional genomics, proteomics and the environmental fields.
The group work on the development of Total Analysis Systems (lab-on-a-chip) based
on three types of biosensors:
(a)
(b)
(c)
A Surface Plasmon Resonance Sensor (SPR)
An integrated Mach-Zehnder Interferometers (MZI) biosensor based on SiCMOS compatible technology
A nanomechanical biosensor based on silicon microcantilevers and its
extension to DNA Biochip
A presentation of each of the devices will be done, covering different aspects as design,
fabrication, operating principle, immobilization procedures of the biological receptor,
flow delivery systems, data acquisition and fields of applications. Our final aim is to
obtain fully-operated prototypes able of performing measures outside the laboratory
environment.
The selectivity of the biosensors is achieved through specific receptors (mainly
antibodies and DNA) immobilized onto the device surface. For functional genomic
applications our aim is the development and fabrication of a nanobiochip based on
microcantilevers with enough sensitive for DNA hybridisation detection, using methods
for oligonucleotide immobilization at nanometer scale on the sensor surface. For
environmental applications, target pollutants we are interested on are pesticides (as
DDT), biocide agents and endocrine disruptors compounds.