Download Osmotic Fragility And Non-Protein Thiol Oxidation By Mentha

Osmotic Fragility And Non-Protein Thiol Oxidation By Mentha pulegium L.
Extracts Associated To t-Butyl Hydroperoxide In Human Erythrocytes
Bianchini, M.C.; Gularte, C.A.O.; Tamborena, T.; Fomer, V.; Puntel, R.L.
Laboratório de Bioquímica e Toxicologia de Produtos Naturais e Sintéticos, UNIPAMPA,
RS, Brazil
Excessive production of reactive oxygen species (ROS) may lead to a condition of
oxidative stress which is a common feature in many pathologic processes. Medicinal
plants are usually used to treat many human diseases. Accordingly, aerial parts of
Mentha pulegium L. has been traditionally used in folk medicine to treat several
disorders. Thus, the aim of this study was to analyze, in vitro, the antioxidant properties
of the different extract of Mentha pulegium using human erythrocytes. Erythrocytes were
maintained in agitation with different concentrations of extracts of M. pulegium and/or tbutyl hydroperoxide for 2hs. The non-protein sulfhydryl groups (NPSH), thiobarbituric
acid reactive substances (TBARS), methemoglobin formation, acetylcholinesterase
activity and osmotic (OF) fragility were determined according methods previously
described. The data were analyzed by the one-way or two-way ANOVA followed by post
hoc test when appropriate. The presented results shown that t-butyl hydroperoxide
induced a significant (p<0.05) increase in TBARS levels compared to control. However,
aqueous (1000 µg/mL) and ethanolic (10-1000 µg/mL) extracts significantly protect
against t-butyl hydroperoxide-induced lipid peroxidation (p<0.0001). Additionally, NPSH
levels were not altered by the t-butyl hydroperoxide, being that both extracts significant
(p<0.0001) potentiates NPSH oxidation when associated to t-butyl hydroperoxide.
Similarly, OF was not altered by the t-butyl hydroperoxide being that extracts potentiates
cell lysis (p<0.05). Both methaemoglobin formation and the acetylcholinesterase activity
were not changed by t-butyl hydroperoxide and/or extracts (p>0.05). Altogether, the
results support the antioxidant potential of M. pulegium. However, more studies are
necessary to better understand mechanism(s) involved in the NPSH oxidation and OF
induced by the plant extract associated with t-butyl hydroperoxide.
Word Keys: blood humans, osmotic fragility, Mentha pulegium L
Supported by: CNPq, CAPES, FAPERGS, FINEP and UNIPAMPA