Download Controls Family identification Family

For Investigational Use Only. Not Intended for Use in Clinical Diagnostics.
Operator
Sample ID
22015037
Experiment ID
22015037
Date of Result
Tue May 20 09:24:49 2014
Assay Name
AMR-ve_Genotyping_AT-1
Assay ID
050103
Well Position
---
Software Version
2014-04-08
Device
---
Controls
control
result
explanation
staining control
passed
"Failed" indicates possible problems originating from the
hybridization or the staining procedure (see troubleshooting
section in the manual).
negative control
passed
"Failed" indicates problems possibly originating from the
staining procedure (see troubleshooting section in the manual).
Family identification
marker
classification
explanation
YES
Summary on family affiliation. "YES" indicates that the isolate
belongs to the family Enterobacteriaceae or to the genus Vibrio
spec. "NO" indicates other bacteria. Please note that Proteus
spec. may yield "NO".
classification
explanation
Enterobacteriaceae
Family-specific Genes
marker
gapA
positive
glyceraldehyde 3-phosphate dehydrogenase A (CP000468.1)
ihfA
positive
integration host factor subunit alpha (U00096.3)
hemL
positive
glutamate-1-semialdehyde aminotransferase (U00096.3)
dnaE
positive
DNA polymerase III subunit alpha (U00096.3)
Genes associated with streptogramin A/B resistance
marker
classification
explanation
vatE
negative
acetyltransferase;streptogramin A acetyltransferase;
associated with resistance to streptogramin A (AF242872.1)
ermB
negative
rRNA adenine N-6-methyltransferase, lincosamide and
streptogramin B resistance protein (AB089505.1)
Genes associated with aminoaminoglycoside resistance
marker
classification
explanation
aadA1, aadA2,
aadA4
negative
aminoglycoside adenyltransferase; associated with resistance
to streptomycin, spectinomycin (EU704128.1)
aac3Ia
negative
3-N-aminoglycoside acetyltransferase; associated with
resistance to astromicin; gentamicin; sisomicin (U90945.1)
aac3IVa
negative
3-N-aminoglycoside acetyltransferase; associated with
resistance to apramycin; dibekacin; gentamicin; netilmicin;
sisomicin; tobramycin (EU784152.1)
aac6Ib
negative
aminoglycoside 6'-N-acetyltransferase; associated with
resistance to streptomycin, spectinomycin (M21682.1)
ant2
negative
aminoglycoside (2'') adenylyltransferase; associated with
resistance to dibekacin; gentamicin; kanamycin; sisomicin;
tobramycin (L06418.4)
strA
negative
aminoglycoside-3''-phosphotransferase (locus A); associated
with resistance to streptomycin (EF090911.1)
strB
negative
aminoglycoside-6''-phosphotransferase; associated with
resistance to streptomycin (EF090911.1)
Genes associated with beta lactam resistance
marker
classification
explanation
acc1, acc2
negative
class C beta-lactamase (EF554600.1)
act1
negative
class C beta-lactamase (U58495.2)
cmy
negative
consensus sequence for blaCMY-13, blaCMY-2, blaCMY-24,
blaCMY-35, blaCMY-CFE1, blaCMY-CFE2 (Citrobacter spp.),
blaCMY-Cmur (Citrobacter murliniae), blaCMY-Cwer
(Citrobacter werkmanii), blaCMY-Cyou (Citrobacter youngae),
blaCMY-HG3, blaCMY-HG4
ctxM1, ctxM15
negative
extended spectrum beta lactamase, class A beta-lactamase
(X92506.1), including ctxM15 (HQ202266.1)
ctxM2, ctxM8,
ctxM26
negative
extended spectrum beta lactamases, class A beta-lactamases
(AM040709.1, AF518567.2, AY750914.2)
ctxM9
negative
class A beta-lactamase (AF174129.3)
dha1
negative
class C beta-lactamase (EF406115.1)
blaFOX
negative
consensus sequence for blaFOX genes (AJ703795.1)
len1
negative
class A beta-lactamase (AY743416.1)
blaMOX
(consensus)
negative
consensus sequence for all blaMOX genes
blaMOX-CMY9
negative
class C beta-lactamase, extended-spectrum beta-lactamase
precursor, associated with resistance to cephalosporins
(AF381617.1)
oxa1
negative
beta-lactamclass D beta-lactamase OXA-1 (AY458016.1)
oxa2
negative
beta-lactamase OXA-2; oxacillinase (U63835.1)
oxa7
negative
class D beta-lactamase OXA-7 (AY866525.1)
oxa9
negative
beta-lactamase OXA-9 (M55547.1)
per2
negative
class A beta-lactamase PER-2; extended-spectrum betalactamase (X93314.1)
pse1
negative
carbenicillinase (Z18955.1)
blaSHV
negative
class A beta-lactamase - consensus sequence for blaSHV
genes, including extended-spectrum beta-lactamases
(EF035566.1)
blaTEM
negative
class A beta-lactamase - consensus sequence for blaTEM
genes, including extended-spectrum beta-lactamases
(Y12694.1)
Genes associated with chloramphenicol resistance
marker
classification
explanation
cmlA1
negative
chloramphenicol transporter (EF113389.1)
catA1
negative
chloramphenicol acetyltransferase (group A) (V00622.1)
cat3
negative
chloramphenicol acetyltransferase (type III) (AJ249249.1)
catB3
negative
chloramphenicol acetyltransferase (group B) (AJ009818.1)
floR
negative
florfenicol export protein (AF252855.1)
catB8
negative
chloramphenicol acetyltransferase (AF227506.1)
Genes associated with macrolide resistance
marker
classification
explanation
ereA
negative
type I erythromycin resistance (AY183453.1)
ereB
negative
type II erythromycin resistance (AB207867.1)
Genes associated with quinolone resistance
marker
classification
explanation
qnrA1
negative
quinolone or fluoroquinolone resistance protein (AY931018.1)
qnrB
negative
quinolone or fluoroquinolone resistance protein (AB281054.1)
qnrS
negative
quinolone or fluoroquinolone resistance protein (AM234722.1)
Genes associated with sulphonamide resistance
marker
classification
explanation
sul1
negative
dihydropteroate synthetase type 1 (AJ698325.1)
sul2
negative
dihydropteroate synthetase type 2 (DQ464881.1)
sul3
negative
dihydropteroate synthetase type 3 (AJ459418.2)
Genes associated with tetracycline resistance
marker
classification
explanation
tetA_11
negative
tetracycline resistance protein A, tetracycline efflux protein
(CP000971.1)
tetB_11
negative
tetracycline resistance protein A, class B (V00611.1)
tetC_11
negative
tetracycline resistance protein A, class C (EU751612.1)
tetD_1
negative
tetracycline resistance protein A, class D (X65876.1)
tetE_11
negative
tetracycline resistance protein A, class E (L06940.1)
tetG_11
negative
tetracycline resistance protein A, class G (AF261825.2)
Genes associated with trimethoprim resistance
marker
classification
explanation
dfrA1_21
negative
dihydrofolate reductase type I (AJ884723.1)
dfrA7_11
negative
dihydrofolate reductase type 7 (AB161450.1, AM237806.1)
dfr12_11
negative
dihydrofolate reductase type 12 (AB154407.1)
dfr13_11
negative
dihydrofolate reductase type A13; dihydrofolate reductase type
A21 (Z50802.3)
dfrA14_21
negative
dihydrofolate reductase type 14 (AJ313522.1)
dfrA15_1
negative
dihydrofolate reductase type 15 (Z83311.1)
dfrA17_11
negative
dihydrofolate reductase type 17 (AF169041.1)
dfrA19_1
negative
dihydrofolate reductase type 19 (AJ310778.1)
dfrV_21
negative
dihydrofolate reductase type 5 (AB188269.1)
Genes associated with integrases
marker
classification
explanation
int1
negative
class 1 integrase (AY260546.3)
int2
negative
class 2 integrase (AY183453.1)