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Some powerpoint slides shown during CHEM 641 class 9/12/07
Primary sequence
Human hemoglobin beta-subunit (FASTA format)
>gi|4504349|ref|NP_000509.1| beta globin [Homo
sapiens]
MVHLTPEEKSAVTALWGKVNVDEVGEALGRLLVVY
PWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLG
AFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRL
LGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVAN
ALAHKYH
Primary Sequence
Secondary Structure
-helix
-helix
double headed arrows indicate hydrogen bonds
H
O
N
O
C
N
R
H
R
H
C
N
O
O
R
H
C
N
C
N
R
H
O
R
H
O
C
N
O
C
N
R
H
R
H
C
N
O
O
C
C
N
R
H
anti-parallel -sheet
H
O
N
N-term
C
N
R
H
R
H
C
O
C
N
R
H
R
N
C
O
O
H
R
O
H
R
O
N
C
N
H
C
C-term
C
N
C
N
N
R
C
O
O
H
R
O
H
R
parallel -sheet
H
O
N
N-term
C
N
R
H
H
O
N
N-term
C
N
R
H
R
H
C
N
O
R
H
C
N
O
O
C
N
R
H
O
C
N
R
H
R
H
C
N
O
C
O
R
H
N
O
C
O
C-term
R
C
R
R
C-term
Secondary Structure
Tertiary Structure
Tertiary Structure
Quaternary Structure
ht-ADH from
B. stearothermophilus
is a homotetramer
Hemoglobin and Myoglobin Quaternary Structure
Hb 22-heterotetramer
Mb – single subunit
(tertiary structure only)
Click picture
to right for Hb
dynamics movie
Classes of Globular Protein Folds
1) Anti-parallel -helix
2) Parallel -sheet -- motif
3) Anti-parallel b-sheet
4) Metal / disulfide rich