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Directions for Use DNA Molecular Weight Markers Code Description Size K180-250UL DNA Molecular Weight Marker,100 bp Ladder 250 µL K181-500UL DNA Molecular Weight Marker,1 kb Ladder 500 µL N550-300UL Ready Ladder™, 100 bp DNA Marker 300 µL N551-600UL Ready Ladder™, 1 kb DNA Marker 600 µL N746-100RXN Ready Ladder™, 50 bp DNA Marker 1,000 µL N854-600UL EZ- Vision® 1 kb DNA Ladder 600 µL N856-300UL EZ Vision® 100 bp DNA Ladder 300 µL General Information VWR Life Science AMRESCO offers 50 bp, 100 bp and 1 kb DNA ladders for determination of double-stranded DNA sizes on agarose or acrylamide gels. The 50 bp ladder consists of 10 fragments ranging from 50 to 500 bp. The 100 bp ladder contains 13 double-stranded DNA fragments ranging from 100 to 3000 bp. The 1 kb ladder consists of 11 double-stranded bands with molecular weights between 500 and 10,000 bp. The intensity of individual bands varies to facilitate easy identification. Ladders are available in three formulations: A buffered aqueous solution that is mixed with loading buffer before applying to gel (K180, K181) Ready Ladder™: A pre-mixture of DNA marker and loading buffer that can be applied directly to the gel (N550, N551, N746) EZ-Vision® DNA Ladders: A pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualization dye that can be applied directly to the gel (N854, N856) Storage/Stability Store frozen (0 to -20ºC). Stable for at least 1 year. Product Use Limitations For research use only. Not for therapeutic or diagnostic use. AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139 Directions for Use Product Details Code Number of Fragments Size Range (bp) Volume (µL) Supplied N746 N550 10 13 50-500 100-3,000 1000 300 100 50 Load Volume (µL) Per Well 6 6 2X Loading Buffer (µL) DNA Staining Required N/A* N/A* Yes Yes N856 13 100-3,000 300 50 6 N/A* No** K180 13 100-3,000 250 50 5 5 Yes K181 11 500-10,000 500 100 5 5 Yes Number of Lanes N551 11 500-10,000 600 100 6 N/A* Yes N854 11 500-10,000 600 100 6 N/A* No** * Ready Ladder™ and EZ-Vision® products are supplied ready-to-use and are to be loaded into wells directly from the supplied tubes. **EZ-Vision® Ladders are a mixture of DNA, loading buffer and a non-toxic, non-mutagenic fluorescent DNA dye. These ladders can be seen immediately after electrophoresis, without additional staining. N746 – Ready Ladder™, 50 bp DNA Marker Fragment Size (bp) 500 450 400 350 300 250 200 150 100 50 DNA Amount (ng) Per 10 µL Volume 79 67 59 55 49 76 33 22 31 29 Ready Ladder™, 50 bp DNA Marker resolved on a 2% TAE/Agarose I™ gel AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139 Directions for Use K181 – DNA Molecular Weight Marker, 1 kb Ladder N551 – Ready Ladder™, 1 kb DNA Marker N854 – EZ- Vision® 1 kb DNA Ladder Fragment Size (bp) 10,000 8,000 6,000 5,000 4,000 3,000 2,500 2,000 1,500 1,000 500 Ready Ladder™, 1 kb DNA Marker resolved on a 1% TBE/Agarose II™ gel AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139 Directions for Use K180 – DNA Molecular Weight Marker, 100 bp Ladder N550 – Ready Ladder™, 100 bp DNA Marker N856 – EZ- Vision® 100 bp DNA Ladder Fragment Size (bp) 3,000 1,000 1,500 1,000 900 800 700 600 500 400 300 200 100 Ready Ladder™ 100 bp DNA Marker resolved on a 1% TAE/Agarose II™ gel AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139 Directions for Use For Technical Support Toll Free: 1-800-610-2789 (USA & Canada) Fax: (440) 349-0235 Email: [email protected] AMRESCO, LLC A VWR Company Corporate Headquarters 28600 Fountain Parkway Solon, Ohio USA 44139-4300 Tel: 440/349-1199 Fax: 440/349-1182 www.amresco-inc.com DNA Molecular Weight Markers ZY0591 Rev. 1 12/2015 © Copyright 2010 by AMRESCO, LLC All Rights Reserved. AMRESCO® is a registered trademark of AMRESCO, LLC AMRESCO, LLC Corporate Headquarters, 28600 Fountain Parkway, Solon, OH 44139