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Cell wall Extract of Mycobacterium bovis BCG
as an immunotherapeutic agent for bladder
cancer treatment
J.Kanimozhi , G.Nirmaladevi
Department of Immunotechnology, Mediclone Academy of Biosciences, Chennai.
Phone 9962602390
Fax
[email protected]
Abstract
Bladder cancer is the fourth most common tumor in men and the eighth most common in
women.BCG (Bacille Calmette - Guerin) vaccine which is derived from a standard strain of
Mycobacterium bovis is a potent immunostimulant, and has been used for the treatment of bladder
cancer, the present work is focused on the use of Tuberculin PPD and CWE (Cell Wall Extract)
obtained from M.bovis (BCG) for the treatment of bladder cancer. Tuberculin Purified Protein
derivative (PPD) used to demonstrate delayed type hypersensitivity and it is a protein made from
the heat treated products of growth and lysis of the tubercle bacillus (Mycobacterium bovis). It is
prepared from the water soluble fraction obtained by heating in steam and subsequently filtering
cultures of the mycobacteria grown in a suitable liquid medium. The protein fraction of the filtrate
is separated by Ammonium Sulphate precipitation, washed and redissolved. The aim of the study
was to investigate antitumor activity of PPD and cell wall extract in vitro. In the in vitro
experiments, NBT -II rat, urinary bladder tumor and Sp2/o mouse myeloma tumor cell lines were
used. The cells were cultured with 50 to 250 µg of two different batches of PPD or CWE for 24 h,
and cytotoxicity assay was performed with the use of crystal violet staining technique. The
biological activity of PPD and CWE was studied by 0.1 ml intradermal injection of PPD and CWE
in sensitized guinea pigs. The protein pattern of PPD and CWE was studied in 10% non reducing
SDS PAGE with silver staining. The results obtained in the in vitro study recommends that PPD
can be used as a therapeutic tool.
Keywords
BCG, PPD, Bladder tumor
Abbreviations
BCG Bacillus Calmittee Guerin
PPD Purified Protein Derivative
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