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THE CORRECTION OF THE UV-INDUCED MUTAGENESIS BY
EXTRACT FROM KIWI FRUIT
Akbarova Gunay
Khazar University, Baku, Azerbaijan
It were done experiments on the Escherichia coli cells with normal genotype (B/r
WP2 K-12 and AB 1157) and their derivatives with defects in the reparation genes:
1885 AV (uvrB¯), BHL 1 (rec A¯), JC 5519 (rec BC¯), JC 9239 (rec F¯) and SM 561
(lex A¯) to identify the characteristics of the correction of the UV-induced mutagenesis
by kiwifruit extract.
There was mutagenesis decreased by 0,001% extract in UV-irradiated cells as
wild type and rec BC¯cells with a high efficiency and in recA¯ lexA¯cells with
substantially reduced efficiency. The efficiency of mutagenesis modifications
practically absent in uvrB¯ and rec F¯ mutants. Thereby the management of resistant of
E.coli cells to mutagenic UV-radiation on the given stage of the mutation process is
largely due to the state of activity of the uvr В и rec F genes products and relatively less
- rec A and lex A genes products.
The modifying effect of kiwifruit extract at the final stages of the mutagenesis is
realized in the interaction between the two main ways of eliminating of induced primary
damages of DNA. One of them is related to the state of the activity of the error-free
DNA repair system, which is under the control of the constitutive enzyme of the
excision and post-replicate repair: products of uvr B (uvr AB-dependent exonuclease),
rec F gene (reparations, but not recombination function of the unidentified product), rec
BC gene (UV-induced endonuclease) and is always active of one of the two centers of
recA gene (protein RecA). Another way depends on the allelic state of lex A genes
(protein Lex A) and the second center of rec A gene and is associated with the
suppression of flowing with errors the inducible DNA repair system. Its important
feature is that increasing the viability of cells carrying DNA damage, it also increases
the total number of mutations.
It indicates that the related part of kiwifruit extract in two main process of
elimination of the primary damage of the DNA determines the sufficiently high
efficiency of modifications of induced mutagenesis at the final stages of mutations
formation.