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Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics Services > In Vivo Xenograft Services Xenografting Xenografting is the transplantation of tissue from one species into another. Xenografting has been established as benchmark studies in pre-clinical cancer research. Typically, immunodeficient mice serve as hosts for a wide variety of human tumors, effectively serving as models for human subjects. Xenografting a complete and accurate study of tumor growth and the activity of drug administration. Xenografting tumor cells into mice has advanced pre-clinical cancer research significantly www.acris-antibodies.com Altogen Labs 11200 Manchaca Road #203 Austin TX 78748 USA Telephone 512 433 6177 email [email protected] Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics Services > In Vivo Xenograft Services Advantages of Xenografting Develop new therapeutic agents Xenotransplantation is the transplantation of living cells, tissues or organs from one species to another. quickly, efficiently and cost effectively Evaluate the efficacy and toxicity of potential therapeutic agents Evaluate target compound activity using in vivo system (human cells) Predict cytotoxicity of cancer drugs Altogen Labs 11200 Manchaca Road #203 Austin TX 78748 USA Telephone 512 433 6177 email [email protected] Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics Services > In Vivo Xenograft Services Xenograft Study Pipelines Pipeline Xenograft Assay I Our staff designs the study based on your project requirements and objectives. Leveraging years of knowledge and experience, Altogen Labs guarantees an optimized experimental approach with respect to mice selection, cell lines, inoculation, and dosage. Pipeline Xenograft Assay II Testing a Compound The client provides a compound and cell line to be researched. Our staff will establish a xenograft animal model and test compound activity and how it affects tumor growth. Pipeline Xenograft Assay III Representative data of a xenograft compound test study Nature (2010) Sep 30;467(7315):596-9 Testing a Cell Line The client provides a cell line and our staff tests its ability to establish tumor growth in standard immunodeficient mice. Altogen Labs 11200 Manchaca Road #203 Austin TX 78748 USA Telephone 512 433 6177 email [email protected] Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics Services > In Vivo Xenograft Services Compound Testing Objectives How does the test compound affect tumor growth? Does the test compound inhibit tumor invasion or metastasis? Localization of test compound in the tumor? Define drug characteristics: Bioavailability Toxicity Efficacy Potency Is the cell line tumorigenic? Changes in gene expression upon treatment or gene manipulation How does gene manipulation in a cell line tumor growth, invasiveness, and metastasis Xenograft studies can rigorously characterize candidate drug efficacy and pharmacokinetic properties http://news.mit.edu Altogen Labs 11200 Manchaca Road #203 Austin TX 78748 USA Telephone 512 433 6177 email [email protected] Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics Services > In Vivo Xenograft Services Basic Xenograft Study Xenograft studies can be designed to: Identify lead compounds Optimize dose schedules Identify combination strategies Altogen Labs 11200 Manchaca Road #203 Austin TX 78748 USA Telephone 512 433 6177 email [email protected] Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics Services > In Vivo Xenograft Services Xenograft Service Options Use in-house or customer-provided cell lines 150 in-house cell lines available Begin testing a compound immediately after injecting mice with tumor cells (unstaged) or once the tumors reach a certain size (staged) Assign mice to groups randomly or stratify them by body weight, tumor size, disease factors, or any combination of parameters Administer test compounds by any of the following methods: topically, IP, IV, SC, PO, high pressure tail vein, osmotic minipumps (SC or IP), SC drug pellets, or in the food or water Snap freeze harvested tumors or fix them for histology or other specialized assays Analyze blood chemistry at various times throughout the study MCF-7 cells LNCap cells jax.org Altogen Labs 11200 Manchaca Road #203 Austin TX 78748 USA Telephone 512 433 6177 email [email protected] pathpedia.com Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics Services > In Vivo Xenograft Services Growth Rates for Different Cell Lines Altogen Labs 11200 Manchaca Road #203 Austin TX 78748 USA Telephone 512 433 6177 email [email protected] Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics Services > In Vivo Xenograft Services Sample Cell Lines Available Cell Line Tissue Disease Caki-2 Kidney Clear Cell Carcinoma PANC-1 Pancreas Epithiloid Carcinoma SK-MEL-2 Skin Malignant Melanoma SK-OV-3 Ovary Adenocarcinoma U-87 MG Brain Glioblastoma/Astrocytoma A431 Epidermis Epidermoid Carcinoma SW-480 Colon Adenocarcinoma HeLa Cervix Adenocarcinoma Altogen Labs has ~150 cell lines available for xenograft animal studies Complete listing available at altogenlabs.com/xenograft-models Altogen Labs 11200 Manchaca Road #203 Austin TX 78748 USA Telephone 512 433 6177 email [email protected] Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics Services > In Vivo Xenograft Services Drug Administration Variables Route of dose: Intratumoral Intramuscular Oral Intravenous Intratracheal Subcutaneous Intraperitoneal Frequency of dose Duration of dose Several routes of drug administration can be explored in a Xenograft model www.patient-derived-xenograft-services.com Altogen Labs 11200 Manchaca Road #203 Austin TX 78748 USA Telephone 512 433 6177 email [email protected] Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics Services > In Vivo Xenograft Services Data Endpoints Estimated tumor volume (L x W2 /2) Mean/median time to specified tumor volume Survival time to specified tumor volume Tumor doubling time Tumor growth inhibition (TGI) Tumor growth delay (TGD) Increased life span (ILS) Tumor cell kill rate A549 (Lung Carcinoma) Xenograft Study Altogen Labs 11200 Manchaca Road #203 Austin TX 78748 USA Telephone 512 433 6177 email [email protected] Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics Services > In Vivo Xenograft Services Expanded Xenograft Services Clinical observations daily Haematology analysis (blood cell count) Clinical chemistry profile (of urine and plasma) Assessment of metastasis Characterization of the tumor (necrosis, etc) Tumoral tissue will be banked and preserved for further analysis Cryo-preservation (for cell culture) Formalin fixed tumors and tissues (for histopathological and immunostaining analysis) RNAlater fixed tumors and tissues (for RNA isolation and gene expression analysis) Our services include expanded tumor cell characterization and preservation cryostuff2.com Altogen Labs 11200 Manchaca Road #203 Austin TX 78748 USA Telephone 512 433 6177 email [email protected] Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics Services > In Vivo Xenograft Services Altogen Labs’ team of scientists carries extensive experience in Xenograft model research Altogen Labs is compliant as a Good Laboratory Practices (GLP) environment, which is required by the FDA for for preclinical research. Our Altogen Labs’ Xenograft services are extensive, rigorous, and flexible. Altogen Labs can partner with you for any Xenograft research project. Photo credit: wisegeek.com Contact us to discuss Xenograft model details, timeline estimates, and price quotes! Altogen Labs 11200 Manchaca Road #203 Austin TX 78748 USA Telephone 512 433 6177 email [email protected]