Download Maija Vuoriluoto - Edinburgh Napier University

Control of protein affinity in
bioactive nanocellulose by
passivation with PDMAEMAblock-POEGMA copolymers and
false positive response
Maija Vuoriluoto1, Hannes Orelma1, Leena-Sisko Johansson1, Baolei Zhu2, Mikko
Poutanen3, Andreas Walther2, Janne Laine1, Orlando J. Rojas1
1Bio-based
Colloids and Materials (BiCMat), Department of Forest Products Technology, Aalto University, School of
Chemical Technology, Espoo, Finland
2DWI − Leibniz-Institute for Interactive Materials Research, Aachen, Germany
3Department of Applied Physics, School of Science, Aalto University, Espoo, Finland
Motivation
• Non-specific protein adsorption limits
the accuracy and selectivity of
biosensors
• Multifunctional polymers containing
poly(ethylene glycol) (PEG) molecules
effective in preventing non-specific
surface protein adsorption
• Cellulosic materials are appealing
substrates for biomedical applications
http://technologyinscience.blogspot.fi/2012/12/rapid-diagnostic-tests-types-strength.html
http://www.early-pregnancy-tests.com/inpregtesstr.html
http://uk.clearblue.com/clearblue-pregnancy-tests-range/clearblue-plus-pregnancy-test
2
Structure of PDMAEMA-blockPOEGMA copolymers analyzed
RAFT polymerization of:
PDMAEMA = poly(2-(dimethylamino)ethyl
methacrylate)
•
Quaternized by methyl iodide
POEGMA = poly(oligo(ethylene glycol)methyl ether
methacrylate)
•
Highly hydrophilic due to PEG units
+ +
200
Monomers
PDMAEMA
150 + +
POEGMA
100
50
0
Block copolymer
adsorption
POEGMA
Block copolymer
adsorption
PDMAEMA
+ + - + + -+ +
+ +
COO COO COO- COO-
TEMPO-CNF
3
Reduction in human IgG adsorption on
TEMPO-CNF with blocking agents
14.0
Adsorbed hIgG
(mg/m²)
12.0
10.0
Blocking efficiency %
15 %
23 %
8.0
6.0
4.0
2.0
0.0
75 %
96 %
100 %
88 %
96 %
85 %
96 %
Adsorbed amount
after blocking with
4
Thank you!