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Control of protein affinity in bioactive nanocellulose by passivation with PDMAEMAblock-POEGMA copolymers and false positive response Maija Vuoriluoto1, Hannes Orelma1, Leena-Sisko Johansson1, Baolei Zhu2, Mikko Poutanen3, Andreas Walther2, Janne Laine1, Orlando J. Rojas1 1Bio-based Colloids and Materials (BiCMat), Department of Forest Products Technology, Aalto University, School of Chemical Technology, Espoo, Finland 2DWI − Leibniz-Institute for Interactive Materials Research, Aachen, Germany 3Department of Applied Physics, School of Science, Aalto University, Espoo, Finland Motivation • Non-specific protein adsorption limits the accuracy and selectivity of biosensors • Multifunctional polymers containing poly(ethylene glycol) (PEG) molecules effective in preventing non-specific surface protein adsorption • Cellulosic materials are appealing substrates for biomedical applications http://technologyinscience.blogspot.fi/2012/12/rapid-diagnostic-tests-types-strength.html http://www.early-pregnancy-tests.com/inpregtesstr.html http://uk.clearblue.com/clearblue-pregnancy-tests-range/clearblue-plus-pregnancy-test 2 Structure of PDMAEMA-blockPOEGMA copolymers analyzed RAFT polymerization of: PDMAEMA = poly(2-(dimethylamino)ethyl methacrylate) • Quaternized by methyl iodide POEGMA = poly(oligo(ethylene glycol)methyl ether methacrylate) • Highly hydrophilic due to PEG units + + 200 Monomers PDMAEMA 150 + + POEGMA 100 50 0 Block copolymer adsorption POEGMA Block copolymer adsorption PDMAEMA + + - + + -+ + + + COO COO COO- COO- TEMPO-CNF 3 Reduction in human IgG adsorption on TEMPO-CNF with blocking agents 14.0 Adsorbed hIgG (mg/m²) 12.0 10.0 Blocking efficiency % 15 % 23 % 8.0 6.0 4.0 2.0 0.0 75 % 96 % 100 % 88 % 96 % 85 % 96 % Adsorbed amount after blocking with 4 Thank you!