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Thrombin Induces the Redistribution and Acute Release of
Tissue Factor Pathway Inhibitor From Specific Granules
Within Human Endothelial Cells in Culture
by Cristina Lupu, Florea Lupu, Ulla Dennehy, Vijay V. Kakkar, and Michael F. Scully
Arterioscler Thromb Vasc Biol
Volume 15(11):2055-2062
November 1, 1995
Copyright © American Heart Association, Inc. All rights reserved.
Immunofluorescent localization of TFPI in resting human endothelial cells in culture.
Cristina Lupu et al. Arterioscler Thromb Vasc Biol.
1995;15:2055-2062
Copyright © American Heart Association, Inc. All rights reserved.
Immunofluorescent visualization of TFPI distribution within human endothelial cells in culture
after thrombin stimulation. a, Immunostaining of EA.hy926 for cell surface–exposed TFPI;
arrows, maximum intensity of fluorescence. b and c, Vertical optical sectioning of cell: as
indicates apical surface; bs, basal side.
Cristina Lupu et al. Arterioscler Thromb Vasc Biol.
1995;15:2055-2062
Copyright © American Heart Association, Inc. All rights reserved.
Quantitative estimation of endothelial cell TFPI release after thrombin stimulation. a, Timedependent release of TFPI antigen in the EA.hy926 medium. b, Effect of thrombin concentration
on TFPI antigen release, measured both in the cell medium (⋄) and lysates (□). c, ELISA assay on
cell monolayers. d and e, TFPI anticoagulant activity exposed on the endothelial cell surface as a
function of thrombin action time and concentration. f, Effect of anti-TFPI antibody on surfaceexpressed TFPI activity.
Cristina Lupu et al. Arterioscler Thromb Vasc Biol.
1995;15:2055-2062
Copyright © American Heart Association, Inc. All rights reserved.
Effect of hirudin and anti-thrombin receptor antibody on TFPI release from thrombin-stimulated
EA.hy 926 cells. a and b, Effect of hirudin-inactivated thrombin on TFPI antigen release,
determined in both cell medium and lysates. c and d, Effect of anti-thrombin receptor antibody
on the TFPI anticoagulant activity, either released in the cell medium or exposed on the cell
surface.
Cristina Lupu et al. Arterioscler Thromb Vasc Biol.
1995;15:2055-2062
Copyright © American Heart Association, Inc. All rights reserved.