Download "DOGRISK: A databank of epi, FFQ and analyzed biological samples

DOGRISK
Anna Hielm-Björkman,
DVM, PhD, PI, Docent
DOGRISK
• Post docs of the group:
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Liisa Uusitalo, PhD (human nutrition),
Sara Zaldiv DVM, Phd (genetics),
Ingrid Hang (DVM; GI-diseases)
Ranno Viitmaa (DVM, Neurology)
• PhD students of the group:
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Johanna Roine, MSc(animal breeding and nutrition),
Riitta Seppänen (DVM, dermatology),
Maritsa Palmunen (DVM ; epidemiology),
Liisa Korkalo (Human nutrition, epidemiology).
• Leader: Anna Hielm-Björkman, PhD
DOGRISK: Doing clinical studies, diet
intervention studies, epidemiology,
FFQ, validating outcome measures,
and analyzing biological samples of
phenotyped dogs
Dept. of Equine and Small Animal
Medicine - Fac. Vet Med
[email protected]
On the edge clinical studies with … or
without, human relevance
• Artificial joint implants
• Feacal transplantation
• Epileptic, Neuropathic
pain, Osteoarthritis
medication/treatments
• New animal sedative
pharmacology
• Horse, cow, dog, cat,
rabbit, elephant…to
humans!
Food frequency questionnaire
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Principle: List of foods provided, frequencies asked
Web-based, 47 foods, specifications in drop boxes
5 frequency options (never---daily or almost daily)
Is validated against an other method
• Use in: Case control setup, logistic regression
Pros
Cons
Long-term intake
Memory-dependent
Large study populations
Less detailed
Diet-disease associations
Amounts crude or unknown
Pet dogs as an animal model for studying dietenvironment-disease associations
Nutritional epidemiology in canine population combines some of the
advantages of two research traditions:
Animal experiments
Human epidemiology
Short follow-up times
Ethical considerations
More monotonous diets
Shared environment
Feeding in human control
Similar health problems
Phenotyping… with numbers
• Diagnostic tools: Clinical examination, lameness exam, force plate, carpet,
scales, x-ray, MRI, CT, thermography…
• Validated indexes: HCPI, F-CSI, neuro (FinFun),… CADESI, CIBDAI, VAS
Taking and using biological samples
• Blood, urine, feces,
biopsies, (saliva), hair…
• Creating reference
values
• Creating a sample bank,
- mostly for own use
• Phenotyped samples
• Time for
anamnesis
Metabolomics
• Analyses were done in FIMM
Metabolomics Unit
• Serum samples from 21 dogs and urine
samples from 8 dogs (unpublished)
Trace elements, vitamins and fatty acids
• Analyses were done in Mineraalilaboratorio MILA
Oy
– Trace elements from whole blood (Li-He)
– Vitamins A, E, D from serum samples
– Fatty acids from EDTA plasma
1.6
1.4
7.5
8.0
8.5
1.5
9.0
Fe
Mg
9.5
1.7
10.0
1.8
10.5
1.9
11.0
• Would need: lab for Vit B12, K2, different A,D and
E vitamins/provitamins
K
R
K
R
• 16 RNA samples from skin biopsies have
been sent to FIMM Sequencing Unit
– RNA sequencing
• qPCR in YES
– RNA from skin biopsies
• To validate the results from RNA sequencing
– RNA from blood samples
• Evaluation of immune response using a candidate
gene approach
Diet intervention studies in dogs
Gut microbiota:
BEFORE diet intervention
(DI):
Firmic: p=0.070
Fusobact:p=0.086
Bacterioidetes: p=0.520
(n=26)
(n=16)
AFTER DI:
Firmic: p<0.001**
Fusobact:p<0.001**
Bacterioidetes: p=0.012*
Dogs given an only 100% raw or dry food diet for 4-5 months, before and after DI
(unpublished)
Diseases we work with now…
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Osteoarthritis
Atopy / allergy/ (food sensitivity)
IBD (~Chron’s disease, Ulcerative colitis)
Epilepsy
Hypothyroidism (in dogs)
Hyperthyroidism (in cats)
Cancer…
…..(diabetes, heart diseases, lever and kidney
diseases, eye diseases….48)
DOGRISK goes cancer…
• Sniffing dogs sniffing cancer from urine samples
• Together with Wise nose sniffer dog association,
East Finland University, HUS – Dept of Oncology
and Cancer clinic, Cancer society of Finland, Small
innovation firms and start-ups.
• Validating the dogs
• Training for different diseases
• http://www.youtube.com/watch?v=FaK79osKYzs
Ideas…towards innovations…
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New pharmacological or nutraceutical products
New registered validated outcome measures
New quick-tests in analyzing
New apps for canine and human health
Diagnosing via currents of the body
Treating via light or a sound
Mobilizing the inner healing in the body
• E-mail: [email protected]
Tel: +358-(0)44-3270462
Thank you!