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Effect of Feed Pellet Characteristics on Growth and Feed Efficiencies of the Largemouth Bass James H. Tidwell and Shawn D. Coyle Kentucky State University Aquaculture Research Center Frankfort, K.Y. USA Largemouth Bass Production >500,000 kg/year in the U.S. $8.50-12.00/kg Live Weight Foodfish Sold live to oriental markets in Toronto, Chicago… Sportfish Uses: Rehabilitative stocking, Managed trophy fisheries, "Put and Take" fisheries Reducing Feed Cost The largemouth is a strict predator. Often fed salmonid diets based more on availability than nutritional suitability. ► >35% ► Diets ► KSU of production cost is feed cost contain high levels of fish meal (>35%) series of studies to develop species specific, cost effective diets. Study 1 Fish Meal Replacement Poultry meal w/ no fish meal Average weight (g) 60 55 a a 50 a b 45 40 c 35 30 Control Poultry w/out FM BM/CGM w/out FM Poultry & 7.5% FM BM/CGM & 7.5% FM Successfully replaced 100% of fish meal with poultry by-product meal Study 2 –Alternatives to Fish Oil These data indicate vegetable based oils can replace fish oil in diets with no reduction in growth. Average weight (g) 100 a 95 a 90 85 a a a a 80 75 70 65 60 Fish oil Corn oil Sunflower oil Linseed oil DHA ARA Study 3 Impact of Carbohydrates Most carnivorous species don’t require CHO, and some cannot tolerate high levels, liver necrosis. Harvest Weights 100 400 380 360 340 320 300 280 260 240 220 200 Survival % 95 90 85 80 75 13% CHO 13% CHO 19% CHO 25% CHO 19% CHO 25% CHO Study 4 Pond Verification ► Evaluate low fish meal and least cost formulation diets for under practical pond conditions ► Commercial diet ~40% fish meal. Experimental diets reduced fish meal levels (45, 24, & 8% = least cost) ► Maintained CHO at or under 20%. Produced experimental diets that were slow sink. Growth $450/ton 550 Average Weight (g) 500 450 $700/ton 400 350 300 Control 45% FM 250 23.5% FM 8% FM 200 May June July August Sample-Harvest September October Harvest Feed Cost / KG Gain $1.10 a Cost (US $ / kg) $1.05 $1.00 Reduced feed costs > 30% $0.95 With better growth b $0.90 $0.85 c $0.80 $0.75 c $0.70 $0.65 $0.60 Control 45% FM 23.5% FM 8% FM Study 5 Impact of Pellet Characteristics. 1) Evaluate “Least Cost Formulation” under new grain price structure (ethanol) 2) Evaluate the impact of pellet characteristics: a) Floating vs. Sinking (reduced CHO) b) Using a larger pellet for largemouth Four diets- Testing Float vs. Sink & Size ► Diet 1 Commercial (CTL), 52% protein, 15% lipid as a 1/4” floating pellet. ► Experimental diets were all formulated “Least Cost” (LC) > 45% protein , > 10% fish meal, > 15% lipid, < 20% CHO. ► Diet 2 LC 6.4 mm floating pellet, ► Diet 3 LC 6.4 mm sinking pellet, ► Diet 4 LC 15.2 mm floating pellet. Diet Ingredient Control Float Sink Menhaden Fish Meal - 10 10 Soybean Meal - 16 18.6 Feather Meal - 10 10 Poultry By- P Meal - 16 15 DDG - 0 10 Corn - 10.6 7.8 Menhaden Fish Oil - 9.9 9.0 Wheat Flour - 8.0 3.0 Poultry Blood meal 7.5 7.4 Rice Bran Hi Fat 6.5 8.0 Mineral Mix - 0.75 0.75 Vitamin Mix - 0.25 0.25 DL-Methionine - 0.7 0.7 Relative Feed Cost 1 0.78 0.83 Diet Analysis Treatment % Moisture % Protein % Fat % Fiber % Ash NFE Control 6.6 50.4 19.4 2.6 8.9 12.4 6 mm Float 8.8 44.1 14.6 2.8 7.8 20.3 6 mm Sink 9.1 43.6 18.4 2.6 7.4 18.9 15 mm Float 8.8 44.3 14.7 2.8 7.7 21.8 Materials & Methods ► Twelve 0.04 ha. ponds were stocked with juvenile LMB (185 g) at 9,880/ha ► Each pond was randomly assigned to one of four treatments with three replications/treatment ► Experimental diets were manufactured By Burris Aquaculture, LA. ► All treatments were fed slowly to apparent satiation 1x/day ► The study lasted from May ► to October ponds sampled monthly. ► DO, temp. and pH monitored 2x/day ► Water quality monitored 2x/week ► Treatments were statistically compared using ANOVA (P<0.05) Survival % a a a a Harvest Weights Grams 650 600 a 550 a b b 500 450 400 350 300 Control LC Float LC Large Sink Flesh Proximate Analysis Treatment % Moisture % Protein % Fat Control 6 mm Float 71.3+1.1 19.8+0.3 8.9+1.2 Least Cost 6 mm Float 71.8+0.7 19.3+0.2 8.1+1.0 Least Cost 6 mm Sink 70.2+0.6 19.6+0.2 9.3+0.5 71.0+0.5 19.4+0.2 9.1+0.4 Least Cost 15 mm Float NSD = No significant differences Feed Cost Per Unit Gain ($/kg) $ a bc b c Conclusions ► The shift to a Least Cost formulation resulted in decreased growth for fish fed floating pellets. ► However, when used as a sinking pellet, growth was the same as the commercial control floating pellet. ► Feeding a least cost formulation as a sinking pellet produced a 20% decrease in feed cost per unit gain. ► However, the use of sinking pellets requires slow feeding, close monitoring and may not be suitable for large scale production. Questions