Download Anesthesia

Anesthesia-Mouse
Monitor anesthesia to ensure an appropriate depth. Anesthesia must be deep enough to provide
adequate analgesia to the animal; however, excessive anesthetic depth is detrimental and may result in
the animal’s death. Monitoring cardiovascular and respiratory parameters, temperature, muscle tone,
and muscle reflexes allow the anesthetist to ensure appropriate anesthetic depth. All drugs are subject
to varying availability and cost, and should be well researched prior to addition to protocol. It is also
recommended (and in some cases required) that drugs be kept fresh and shielded from light sources.
Drug Name
Dose
Route of
administration
Halothane
1%-4% to effect
Inhalation
Isoflurane
1%-4% to effect
Inhalation
Sevoflurane
To effect
Inhalation
Acetylpromazine
2-5 mg/kg
IP
Alphaxalone,
alphadolone
(Saffan, Althesin)
Diazepam
10-20 mg/kg
IV
5 mg/kg
IP
Fentanyl, fluanisone
(Hypnorm)
0.4 ml/kg
IM, IP
Fentanyl/ fluanisone
+ diazepam
0.4 ml/kg
5 mg/kg
IP
Ketamine
80-100 mg/kg
IM
Ketamine
100 mg/kg
IP
Ketamine +
acetylpromazine
44-65 mg/kg
0.75-2 mg/kg
IP
Ketamine +
acetylpromazine
30 mg/kg
1 mg/kg
IM
Ketamine +
100 mg/kg
IP
Comments
Unpredictable anesthetic effect following
IP, volume too large for IM
muscle rigidity, pronounced respiratory
depression, 1:10 dilution
sedation, muscle rigidity
sedation only
marked respiratory distress
acetylpromazine
2.5-5 mg/kg
Ketamine +
diazepam
100mg/kg
5 mg/kg
IM
IP
Ketamine +
midazolam
100 mg/kg
5 mg/kg
IP
only light anesthesia
Ketamine +
medetomidine
75 mg/kg
1 mg/kg
IP
Female mice
Ketamine +
medetomidine
40mg/kg
1 mg/kg
IP
male mice
Ketamine +
xylazine
30-100 mg/kg
5-6 mg/kg
IM
excellent relaxation, sedation, analgesia
Ketamine +
xylazine
65-100 mg/kg
10-13 mg/kg
IP
excellent relaxation, sedation, analgesia
Pentobarbital
(Nembutal)
45 mg/kg
IP
1:10 dilution, narrow safety margin,
marked strain difference in response
Pentobarbital
(Nembutal)
50 mg/kg
IP
severe respiratory depression
26-30 mg/kg
IV
short term anesthesia
30 mg/kg
IV
short-term anesthesia, dose dependent
hypothermia and respiratory depression
40-100 mg/kg
IP
Telazol +
xylazine
7.5 mg/kg
45 mg/kg
IM
Telazol +
xylazine
45 mg/kg
7.5 mg/kg
IM
125-300 mg/kg
IP
Propofol (Rapinovet,
Diprivan)
Thiopental
(Penthotal, Trapanal)
Tiletamine/
zolazepam (Telazol)
Tribromoethanol
(Avertin)
long-term anesthesia
1.2% solution, possible peritonitis, serositis