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Microbiology lecture homework 6 1- Capsids are composed of a) DNA b) RNA c) lipids d) proteins 2a) b) c) d) Temperate phages often lyse their host cell all the time integrate their DNA into the host DNA change properties of their hosts both b and c are correct 3a) b) c) d) All phages must have the ability to have their nucleic acid enter the host cell have their nucleic acid replicate in the host cell synthesize RNA molecules both a and b are correct 4a) b) c) d) The tail fibers on phages are associated with penetration attachment protein translation transcription 5a) b) c) d) The phages QB and MS2 are single stranded RNA lytic phages have a large burst size Have double stranded DNA Both a and b are correct 6- True or False T4 phage is single stranded DNA lytic phage. 7- True or false Single stranded RNA lytic phages have a large burst size and attach to F+ pili Host cells. 8- True or False T4 is considered a double stranded DNA virulent phage meaning it is lytic. 9- True or False Transduction is the transfer of DNA from one bacterial cell to a donor bacterial cell. 10- Phage lambda DNA integrates into the E.coli genome at a specific site through a process called a) transduction b) translation c) transcription d) site specific recombination 11- Which enzyme removes the lambda viral DNA from the host DNA once the repressor molecules are removed or no longer active? a) excisase b) ligase c) lipase d) nuclease 12- Which enzyme destroys the host DNA once a cell is infected with a virus? a) DNAase b) RNAase c) Reverse transcriptase d) None of the above 13- True or False The enzyme lysozyme helps a phage break through the bacterial cell wall. 14- The induction of a temperate phage can be achieved by exposing the bacterial cells to ultra violet light. This is probably the result of a) destroying the repressor molecules and allowing RNA polymerase to bind to the promoter region and transcribing a messenger RNA. b) destroying the cell wall of the host c) destroying the virus d) all of the above 15-Viroids a) contain only single stranded RNA and cause disease in plants b) use reverse transcriptase in their replication c) are similar in structure to phages d) can cause human diseases 16-Animal viruses can be classified according to a) lipid composition b) Ribosome count c) Genome type, shape and presence of an envelope d) None of the above 17-True or False Prions are infectious agents that consist of only RNA. 18-True or False An acute infection tends to be localized, short in duration and often checked by the host’s immune system. 19-The enzyme that HIV utilizes to copy its RNA genome into a complimentary DNA strand and create an RNA- DNA hybrid is a) nuclease b) lysozyme c) lipase d) reverse transcriptase 20-True or False Unlike a lambda phage, HIV integrates its genome randomly into the host’s genome. 21-True or False A naked phage can enter by both endocytosis and entry following membrane fusion method. 22-These proteins on enveloped viruses aid in the process of entry a) b) c) d) spike proteins collagen tubulin capsomeres 23-The building blocks of the capsid are a) capsomeres b) glucose c) phospholipids d) none of the above 24-True or False Herpes type I can be considered a latent persistent infection. 25-A method used by animal viruses to exit the host cell is a) budding b) active transport c) binary fission d) nuclear fission 26-Filamentous phages a) always kill the host cell b) are long thin fiber- like phages that can multiply inside the host without killing the host allowing the host to also multiply. c) contain only RNA d) none of the above 27-An example of a single stranded DNA lytic phage is a) M13 phage b) Phi X 174 phage c) T4 phage d) Lambda phage 28-True or False According to our textbook, there are 14 families of RNA containing viruses and 8 DNA containing viruses. 29-The viral capsid together with the nucleic acid that is tightly packed within the protein coat is called a) nucleocapsid b) lipid layer c) lipid raft d) matrix proteins 30-True or False In the carrier state, infectious virions of Hepatitis B can be continually produced and detected in the bloodstream, semen and saliva. 31-True or False HIV disease has features of slow virus infections. 32- All phages must have the ability to a. b. c. d. have their nucleic acid enter the host cell have their nucleic acid replicate in the host both a and b none of the above 33- The polio virus is an example of a. b. c. d. RNA virus DNA virus Both DNA and RNA A prion 34- Animal viruses are divided into a number of families whose names end in A. -virus. B. -viridae. C. -viscous. D. -eieio. . 35-There are _______ families of RNA containing viruses that infect vertebrates. A. two B. five C. seven D. fourteen . 36-The receptors to which animal virus attachment proteins usually bind are A. proteins. B. carbohydrates. C. nucleic acid. D. lipids. E. glycoproteins. 37-When an enveloped virus adsorbs to the host cell with its protein spikes, the virions are taken into the cell by the process of A. penetration. B. production. C. fusion. D. endocytosis. 38-The enveloped viruses typically obtain their envelope A. from the host plasma membrane. B. as they exit the host. C. from a newly constructed viral-derived membrane. D. from the nuclear membrane. E. from the host plasma membrane AND as they exit the host.