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Dry-Milling Facility Hammer mills Yeast Propagation Tank(2) Steam Jet Cook Tube Slurry Tank Flash Tank Liquefaction tank Fermentation Tank(4) Plate & Frame HX Beer Well Degasser Stripper Column Centrifuges Rectifier Column CWR Stripper Column Reboiler To recycle water tank Rectifier Thermo siphon Reboiler 4 Bottle molecules sieve system Thin Stillage Tank 200 Proof Product To dryers or wet feed To syrup tank Byproduct Alternative Uses To Cooker Stripper Column Thin Stillage Beer Bottom (Whole Stillage) Evaporator Syrup Centrifuge Wet Distillers Grain (WDG) Dried Distillers Grain with Solubles (DDGS) Dryer Alternative Uses Fiber Separation Pretreatment Proteins Soluble Fiber Pure cellulsic material Animal supplement Industrial polymers Human dietary food Industrial polymers Ethanol production Oil Extraction Protein Extraction Oil Biodiesel Lignin Oil Extraction Beer Bottom Blender or Grinder Mixing Centrifuge Solvent (Hexane) Top Liquid Phase (Solvent / Oil) Solvent Evaporation Substrates for Protein Extraction Oil Oil Extraction - Composition Hexane (Hexane : Beer Bottom = 3 : 10) Beer Bottom (BB) Moisture : 84.14 % Oil : 11.35 % Protein : 24.74 % Lignin : 7.32 % Starch : 13.01 % Cellulose : 14.60 % Hemicellulose : 24.84 % Other : 4.14 % Oil Extraction Oil Defatted BB Moisture : 85.74 % Protein : 27.91 % Lignin : 8.26 % Starch : 14.68 % Cellulose : 16.47 % Hemicellulose : 28.02 % Other : 4.67 % Protein Extraction : Scheme I Defatted BB Centrifuge Albumin Isolation Liquid Albumin Salt (NaCl) Solution Solid Mixing Aqueous Alcohol (Ethanol) Centrifuge Liquid Globulin Isolation Globulin Solid Centrifuge Mixing Liquid Prolamin Isolation Prolamin Liquid Glutelin Isolation Glutelin Solid Alkali solution (NaOH) Mixing Centrifuge Solid Substrates for Fiber Extraction Protein Extraction : Scheme II Defatted BB Protein solubilization Centrifuge Ethanol NaOH pH control Liquid Solid Protein Isolation Centrifuge Substrates for Fiber Extraction Protein Fiber Separation Substrate from protein extraction Treatment (Acid or alkali) Ethanol Liquid Centrifuge Liquid Fiber Isolation (Precipitation) Solid Centrifuge Insoluble Fiber for Pretreatment Solid Soluble Fiber Pretreatment & Lignin Separation Substrates (Insoluble Fiber) Acid Dilute-Acid Pretreatment pH control Centrifuge Lignin Isolation Centrifuge Liquid Solid Pure cellulosic material for enzymatic hydrolysis Solid Lignin