Download CV - Bigelow Laboratory for Ocean Sciences

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Curriculum Vitae of Roman A. Barco
NSF Postdoctoral Fellow
Bigelow Laboratory for Ocean Sciences
60 Bigelow Drive
P.O. Box 380
East Boothbay, ME 04544
Phone: 207-315-2567
Email: [email protected]
Education
Ph. D.
University of Southern California
Biological Sciences, 2014
Title: The marine, neutrophilic, and chemolithoautotrophic iron-oxidizing bacteria:
insights into the physiology of Zetaproteobacteria and the discovery of novel ironoxidizing Gammaproteobacteria.
Committee: Dr. Katrina Edwards (advisor; deceased), Dr. Kenneth Nealson, Dr.
David Emerson (Bigelow), Dr. Jason Sylvan and Dr. Frank Corsetti
B. S.
California State University - Los Angeles
Microbiology, 2004
Advisor: Dr. Tina M. Salmassi
Appointments
Postdoctoral Researcher, Bigelow Laboratory for Ocean Sciences, August 2015 - present.
Postdoctoral Scholar - Research Associate , University of Southern California, September 2014 present.
Honors
National Science Foundation - Postdoctoral Fellowship in Biology, 2015 - present.
Center for Dark Energy Biosphere Investigations (C-DEBI) - Graduate Student Fellowship, 2011-2013.
USC Wrigley Institute for Environmental Studies - Graduate Student Summer Fellowship, 2011.
USC Outstanding TA Award, 2008.
USC Marine Environmental Biology Tyler Merit Fellowship, 2007.
NSF Center for Environmental Analysis – Centers of Research Excellence in Science and Technology
(CEA-CREST) Graduate Student Fellowship, 2005-2007.
California State University - Los Angeles, Office of Graduate Studies & Research Student Recognition,
11/2006.
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NSF Undergraduate Mentoring in Environmental Biology (UMEB)-CEA-CREST Undergraduate
Student Fellowship (at CSULA), 2003-2004.
Publications
Barco, R.A., Emerson, D., Sylvan, J.B., Orcutt, B.N., Jacobson Meyers, M.E., Ramírez, G.A., Zhong,
J.D., Edwards, K.J. 2015. New insight into microbial iron oxidation as revealed by the proteomic profile
of an obligate iron-oxidizing chemolithoautotroph. Appl. Environ. Microbiol. 81(17):5927-37. doi:
10.1128/AEM.01374-15
Barco, R.A. and Edwards, K.J. 2014. Interactions of proteins with biogenic iron oxyhydroxides and a
new culturing technique to increase biomass yields of neutrophilic, iron-oxidizing bacteria. Front.
Microbiol. 5:259. doi: 10.3389/fmicb.2014.00259
Bennett, S.A., Toner, B., Barco, R.A., and Edwards, K.J. 2014. Carbon adsorption by Fe oxyhydroxide
stalks produced by a lithotrophic iron-oxidizing bacteria. Geobiology. doi: 10.1111/gbi.12074.
Orcutt, B.N., Barco, R.A., Joye, S.B., Edwards, K.J. 2012. Summary of carbon, nitrogen, and iron
leaching characteristics and fluorescence properties of materials considered for subseafloor
observatory assembly. Proc. IODP. 336: doi:10.2204/iodp.proc.336.108.2012.
Singer, E., Emerson, D., Webb, E.A., Barco, R.A., Kuenen, J.G., Nelson, W.C., Chan, C.S., Comolli,
L.R., Ferriera, S., Johnson, J., Heidelberg, J.F., Edwards, K.J. 2011. Mariprofundus ferrooxydans PV-1
the first genome of a marine Fe (II) oxidizing Zetaproteobacterium. PLoS ONE. 6 (9): e25386.
doi:10.1371/journal.pone.0025386.
Barco, R.A., Patil, D.G., Xue, W., Ke, L., Khachikian, C.S., Hanrahan, G., Salmassi, T.M. 2006. The
development of iodide-based methods for batch and on-line determinations of phosphite in aqueous
samples. Talanta. 69: 1292-1299.
Manuscripts In Revision
Barco, R.A., Emerson, D., Orcutt, B.N., Sylvan, J.B., Zhong, J., Edwards, K.J. The proteomic profile
of an obligate iron-oxidizing chemolithoautotroph reveals new insight into microbial iron oxidation. In
revision for Applied and Environmental Microbiology.
Manuscripts In Preparation
Barco, R.A., Ramirez, G.A., and Edwards, K.J. In-situ incubation of iron-sulfide mineral in seawater
reveals colonization by iron-oxidizing Gammaproteobateria and sheath-forming Zetaproteobacteria.
Funding
Funded
Barco, R.A., and Edwards, K.J. Proteomic profiling of neutrophilic, iron-oxidizing Mariprofundus
ferrooxydans, strain PV-1, grown under different iron sources. Center for Dark Energy Biosphere
Investigations (C-DEBI Graduate Student Fellowship); $64,000; August 2011- August 2013.
Barco, R.A. and Edwards, K.J. USC Wrigley Institute Summer Graduate Fellowship; $4400; June
2011 - August 2011.
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Oral Presentations
Barco, R.A. The marine, neutrophilic, and chemolithoautotrophic iron-oxidizing bacteria: insights into
the physiology of Zetaproteobacteria and the discovery of novel iron-oxidizing Gammaproteobacteria.
Defense presentation. April 21st, 2014 at University of Southern California, Los Angeles, CA.
Barco, R.A. Near-shore in-situ study of marine, neutrophilic, iron-oxidizing bacteria. Marine
Environmental Biology Seminar. February 14th, 2012 at University of Southern California, Los
Angeles, CA.
Barco, R.A. In search of biomarkers for iron-oxidizing bacteria (FeOB). NSF STC Site Review
(hosted by Center for Dark Energy Biosphere Investigations). January 30th, 2012 at University of
Southern California, Los Angeles, CA.
Barco, R.A. Near-shore in-situ study of marine, neutrophilic, iron-oxidizing bacteria. Wrigley Summer
Fellows Symposium. September 23rd, 2011 at University of Southern California, Los Angeles, CA.
Barco, R.A. In search of biomarkers for iron-oxidizing bacteria (FeOB). In search of biomarkers for
iron-oxidizing bacteria (FeOB). Marine Environmental Biology Seminar. May 5th, 2011 at University of
Southern California, Los Angeles, CA.
Barco, R.A. Extraction of proteins from a filamentous, neutrophilic, iron-oxidizing bacteria,
Mariprofundus ferrooxydans. Marine Environmental Biology Seminar. March 30th, 2010 at University
of Southern California, Los Angeles, CA.
Barco, R.A. In search of iron-oxidizing proteins in Mariprofundus ferrooxydans, strain PV-1. Marine
Environmental Biology Seminar. March 24th, 2009 at University of Southern California, Los Angeles,
CA.
Posters
Barco, R.A., Zhong, J., Ramirez, G.A., Reese, B., and Edwards, K.J. 2012. Identification of a
membrane cytochrome c from neutrophilic, iron-oxidizing Mariprofundus ferrooxydans, strain PV-1.
American Geophysical Union. San Francisco, CA.
Barco, R.A. and Edwards, K.J. In search of biomarkers for iron-oxidizing bacteria (FeOB). 2011.
American Geophysical Union Fall Meeting, San Francisco, CA.
Barco, R.A., and Edwards, K.J. 2011. Extraction of Proteins from filamentous, iron-oxidizing
Mariprofundus ferrooxydans, strain PV-1. Southern California Geobiology Symposium, University of
Southern California, Los Angeles, CA.
Barco, R.A. and Edwards, K.J. 2010. Protein Extraction Methods from Filamentous Iron-Oxidizing
Zeta-Proteobacteria. NSF - STC Director’s Meeting, Arlington, VA.
Barco, R.A. and Edwards, K.J. 2010. Protein Extraction Methods and Separation of Filaments from
Filamentous Iron-Oxidizing Mariprofundus ferrooxydans, strain PV-1. 110th General Meeting for the
American Society of Microbiology, San Diego, CA.
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Barco, R.A., Sanudo-Wilhelmy, S.A, Edwards, K.E. 2008. Effects of Iron Dissolution Reagents on the
Outer Membrane of Marinobacter aquaeolei. Southern California Geobiology Symposium, University
of Southern California, Los Angeles, CA.
Barco, R.A. and Salmassi, T.M. 2007. Isolation of Arsenic-Oxidizing Thermophiles by a Differential
and Selective Type of Medium in Hot Creek, CA. 107th General Meeting for the American Society of
Microbiology, Toronto, Canada.
Barco, R.A., Moran, J., Rusu, C., Vargas, C., Ammons, C., Reyes, C., Salmassi, T.M. 2005. ArsenicOxidizing Thermus spp. Isolated from Hot Creek, CA. 105th General Meeting for the American Society
of Microbiology, Atlanta, GA.
Barco, R.A., Patil, D., Salmassi, T.M., Hanrahan, G. 2004. Development of a Starch Iodide Method for
the Determination of Phosphite in Natural Waters. American Geophysical Union Fall Meeting, San
Francisco, CA.
Lowe, G.N., Barco, R.A., Mendoza, R., Khachikian, C.S., Salmassi, T.M. 2004. Oxidation of Arsenite
in Bioreactors by Agrobacterium albertimagni strain AOL-15. 104th General Meeting for the American
Society of Microbiology, New Orleans, LA.
Salmassi, T.M., Ayala, E.A., Barco, R.A., Becerra, C.A., Chakhalyan, M.M., Connor, K.M., Cuevas, G.,
Dolmajian, H.K., Hirun, M.S., Lam, R.O., Martinez, A.F., Martinez, J.G., Mendoza, R.Y., Moshkani, S.,
Nguyen, J.Q., Ovasapyan, K., Shin, S., Sok, D., West, K.L., Wong, N.J., Wu, R.C. 2003. Effects of
Elevated CO2 on Soil Bacterial Diversity: A Comparative Study at Mammoth Mountain, CA. 103rd
General Meeting for the American Society of Microbiology, Washington D.C., DC.
Teaching Experience
USC, Spring 2008. Laboratory - General Biology.
USC, Fall 2008. Laboratory - Introduction to Ecology.
USC, Spring 2009. Laboratory - Introduction to Microbiology.
Selected Synergistic Activities
Community Service
Reviewer for Geomicrobiology Journal. Summer 2014.
Participated in the Rising Deep Scholars Program (C-DEBI sponsored). Co-mentored three high
school students from Animo Leadership Charter High School: Anthony Hernandez, Anthony Esparza
and Kevin Sanchez. Assisted the students in analyzing clone libraries and in producing a phylogenetic
tree for a poster. Spring 2013.
Undergraduate Student Mentoring
May 2010 – May 2013. Mentored John Zhong (undergraduate student at USC). John assisted me
with culturing iron-oxidizing bacteria, performing cell counts and with the production of media. John
was awarded a USC Discovery Scholar Medal for work related to one of my supervised projects
involving proteomics and Zetaproteobacteria.