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CURRICULUM VITAE
Dr. Asmat Salim
Current Position:
Present Address:
Home Address:
Home Phone #:
Associate Professor
Dr. Panjwani Center for Molecular Medicine & Drug Research,
International Center for Chemical and Biological Sciences,
University of Karachi, Karachi-75270, Pakistan
Tel: +92 21-4824930, 4824934, 4824936 (Ext: 308).
E. Mail:[email protected]; [email protected]
B-206, Block C, North Nazimabad, Karachi 74700
+ 92 21 36632984
ACADEMIC CAREER
DEGREE
YEAR
DIVISION
SUBJECT(S)
DEPARTMENT/UNIVERSITY
B. Sc. (Hons)
1990
First Class
First position
Biochemistry (major),
Microbiology, Physiology
Department of Biochemistry,
University of Karachi, Pakistan
M. Sc.
1991
First Class
First position
Biochemistry
Department of Biochemistry,
University of Karachi, Pakistan
Ph.D.
2001
Protein Chemistry
HEJ Research Institute of
Chemistry, University of Karachi,
Pakistan
ACADEMIC EXPERIENCE
Ph.D. Thesis: “Structural studies on human crystallins”
Passed the following courses offered as the partial fulfillment of the Ph.D. program:
 Physical Methods of Isolation and Structure Elucidation
 Proteins
 Enzymology
 Strategies in Protein Sequencing
 Protein and Peptide Synthesis
M.Sc. Thesis (Neuropharmacology): "Effect of tryptophan load on stress-induced changes of brain
serotonin in male rats".
PRESENT STATUS
Associate Professor, Dr. Panjwani Center for Molecular Medicine and Drug Research. International
Center for Chemical and Biological Sciences, University of Karachi
PROFESSIONAL POSITIONS
Associate Professor (January 2014 – present)
Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical
and Biological Sciences, University of Karachi.
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Asmat Salim
Assistant Professor (April 2007 – December 2013)
Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical
and Biological Sciences, University of Karachi.
Senior Research Officer (April 2004 – March 2007)
Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical
and Biological Sciences, University of Karachi.
Visiting Scientist (August 2005 – November 2005)
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio.
Research Fellow (August 2004 – July 2005)
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio.
Assistant Professor (October 2003 – April 2004)
Department of Biochemistry, Baqai Medical University, Karachi.
Exam Question Writer (February 2003 – January 2004)
Online Exam Question writer in the field of Biochemistry, Biology and Chemistry, DSK College,
London.
Visiting Faculty (June 2003- July 2003, Summer Course on Bioinformatics)
FAST Institute of Computer Science, National University of Emerging Sciences, Karachi, Pakistan.
Postdoctoral Research Fellow (April 2002 – April 2003)
Bioinformatics and Protein Chemistry (3D structure prediction of proteins by computer based
molecular modeling), Department of Biochemistry, University of Karachi
Teaching Assistant / Cooperative Teacher (August 1993-December 1994)
Department of Biochemistry, University of Karachi, Karachi, Pakistan
CURRENT RESEARCH ACTIVITIES
Undergoing projects and Funding Sources:
1. Funding Agency: Higher Education Commission
Project: Role of preconditioned and genetically modified mesenchymal stem cells in the
regeneration of cardiac tissue
Period: 2 years
Budget approved: Rs.3,534,639/=
2. Funding Agency: Pakistan Science Foundation
Project: Preconditioning of the stem and progenitor cells to increase their cardiomyogenic
potential
Period: 2 years
Budget approved: Rs.813,200/=
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Asmat Salim
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M.Phil./Ph.D. Projects:
Following projects are in progress for the M. Phil. / Ph. D. program in Molecular Medicine:
1. Role of cytokines and growth factors in the stimulation of stem cells for the regeneration of
cardiac tissue
2. Study of differentiation potential of bone marrow mesenchymal stem cells into cardiac cells
3. Study of regenerative potential of various populations of stem cells into cardiomyocytes
4. Study of the effect of free radicals and antioxidants in aging and apoptosis of bone marrow
mesenchymal stem cells
5. Role of GTPases in the differentiation process of bone marrow stem cells to cardiac cells.
Ph.D. awarded
1. Uzma Jabeen
(2011)
2. Kanwal Haneef
(2012)
3. Nadia Naeem
(2012)
4. Irfan Khan
(2013)
5. M. Aleem Akhter
(2013)
6. Anwar Ali
(2013)
Ph.D. submitted
1. Nazia Ahmed
2. Hana’a Iqbal
LIST OF PUBLICATIONS
(Total Impact factor = 53.647)
1. Haneef K, Naeem N, Iqbal H, Khan I, Jamall S, Kabir N, Zahid M and Salim A.
Conditioned hypoxic medium enhances the fusion capability of rat bone marrow
mesenchymal stem cells and cardiomyocytes in a time dependent manner. Accepted
(Molecular Biology Reports).
2. Naeem N, Haneef K, Kabir N, Iqbal H, Jamall S and Salim A. (2013) DNA demethylating
agents, 5-azacytidine and zebularine potentiate the trans-differentiation of rat bone marrow
mesenchymal stem cells into cardiomyocytes. Cardiovascular Therapeutics, 31:201-209.
3. Eijaz S, Salim A and Waqar MA. (2013) Possible Molecular Targets of Cinnamon in the
Insulin Signaling Pathway. Accepted (Journal of Biochemical Technology).
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Asmat Salim
4. Jabeen U and Salim A. (2013) Sequence Analysis and Structure Prediction of Enoyl CoA
Hydratase from Avicennia marina; Implication of Various Amino Acid Residues on
Substrate-Enzyme Interactions. Phytochemistry, 94:36-44.
5. Razwi NZ, Khurshid R, Saleem M, Karim S, Nagra SA and Salim A. (2012) Biochemical
analysis of granzyme H and elucidation of its structurally important residues involved in
substrate and inhibitor binding, J. Chin. Pharm. Sci., 21, 33-39.
6. Ali A, Akhter MA, Haneef K, Khan I, Naeem N, Kabir N and Salim A The effect of
hypoxia on the gene expression levels of angiogenic, cell survival, and cardiomyogenic
factors in rat bone marrow derived mesenchymal stem cells at different time periods after reoxygenation. Submitted (Gene).
7. Jabeen U, Salim A and Abbasi A. (2012) Prediction of Post Translational
Modifications
in
Avicennia
marina
Cu-Zn
Superoxide
Dismutase:
Implication of Glycation on the Enzyme Structure. J. Chem. Soc. Pak. 34, 404-414.
8. Jabeen U, Abbasi A and Salim A. (2011) Predicting the functionally distinct residues in the
heme, cation and substrate binding sites of peroxidase from stress-tolerant mangrove specie,
Avicennia marina. Cell Stress and Chaperones 16, 585-605.
9. Haneef K, Naeem N, Iqbal H, Jamall S, Kabir N and Salim A. (2011) Gene expression
pattern in rat bone marrow mesenchymal stem cells in response to hypoxia. Pak. J. Biochem.
Mol. Biol. 43, 90-3.
10. Jabeen U, Salim A and Abbasi A. (2011) Prediction of enzyme-inhibitor interactions in
Avicennia marina Cu-Zn Superoxide Dismutase: implications of functionally significant
residues in the metal binding sites. Pak. J. Biochem. Mol. Biol. 44, 1-7.
11. Khurshid R, Saleem M, Akhtar MS and Salim A. (2011) Granzyme M: characterization with
sites of post-translational modification and specific sites of interaction with substrates and
inhibitors. Molecular Biology Reports, 38, 2953-60.
12. Gul A, Rahman MA, Salim A and Simjee SU. (2009) Advanced glycation end products in
senile diabetic and nondiabetic patients with cataract. J. Diabetes Complications 23, 343-8.
13. Hashim Z, Ilyas A, Saleem A, Salim A and and Zarina S. (2009) Expression and activity of
Paraoxonase 1 (PON1) in human cataractous lens tissue. Free Radical Biol. & Med. 46,
1089-95.
14. Gul A, Rahman MA, Salim A, and Simjee SU. (2008) Advanced glycation end products in
senile diabetic and nondiabetic patients with cardiovascular complications. AGE 30, 303-9.
15. Gul A, Rahman MA, Hasnain SN, Salim A and Simjee SU. (2008) Could oxidative stress
associate with AGE-products in cataractogenesis? Current Eye Research 33, 669-75.
16. Jiang S, Haider KH, Ahmed RP, Idris NM, Salim A and Ashraf M. (2008) Transcriptional
profiling of young and old mesenchymal stem cells in response to oxygen deprivation and
reparability of the infarcted myocardium. Journal of Molecular and Cellular Cardiology 44,
582-96.
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Asmat Salim
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17. Jiang S, Haider KH, Idris NM, Salim, A and Ashraf M. (2006) Supportive Interaction
Between Cell Survival Signaling and Angiocompetent Factors Enhances Donor Cell
Survival and Promotes Angiomyogenesis for Cardiac Repair. Circulation Res. 99, 776-84.
18. Khurshid R, Salim A and Abbasi A. (2005) Three-dimensional structure prediction of
bovine AP lyase, BAP1: prediction of interaction with DNA and alterations as a result of
Arg176-->Ala, Asp282-->Ala, and His308-->Asn mutations. Biochem. and Biophys. Res.
Commun. 316, 711-7.
19. Rashid A, Khurshid R, Begum M, Raana G, Latif M and Salim A. (2004) Modeling the
mutational effects on calmodulin structure; prediction of alteration in the amino acid
interactions. Biochem. Biophys. Res. Commun. 317, 363-9.
20. Salim A and Zaidi ZH. (2004) Prediction of stability factors at the domain interface of
Human γB crystallin by Homology Modeling. J. Pak. Med. Assoc. 54, 419-22.
21. Salim A, Bano A and Zaidi ZH. (2003) Prediction of possible sites for post translational
modifications in Human Gamma Crystallins: Effect of glycation on the structure of human
gammaB-crystallins as analyzed by molecular modeling. Proteins Structure, Function and
Genetics 53, 162-73.
22. Salim A, Haleem R, Murtaza F, Shameem G, Perveen T and Haleem DJ. (2003) Injected
tryptophan increases brain but not plasma tryptophan level more in stressed rats. Pak. J.
Pharm. Sciences. 16, 51-7.
23. Salim A and Zaidi ZH. (2003) Homology Models of Human Gamma Crystallins: Structural
study of the extensive charge network in gamma crystallins. Biochem. Biophys. Res.
Commun. 300, 624-30.
24. Khurshid R and Salim A. (2000) Amino Acid Sequence Homology and Modelling of
Cystein Protease Cathepsin S. Pak. J. Biol. Sci. 3, 639-41.
PRESENTATIONS/ABSTRACTS

Irfan Khan, Muhammad Aleem Akhter, Nadia Naeem, Anwar Ali, Hana’a Iqbal, Asmat
Salim. Rap1 activated mesenchymal stem cells transplantedin infarcted myocardium
improve heart function. Poster presented at the“11th Biennial Conference of the Pakistan
Society for Biochemistry and Molecular Biology (PSBMB)”, held in Lahore, Pakistan (Nov.
25-28, 2013).

Asmat Salim, Nadia Naeem, Kanwal Haneef, Anwar Ali, Muhammad Aleem Akhter, Irfan
Khan, Hana’a Iqbal Nurul Kabir and Siddiqua Jamall. Chemical and Genetic Modification
of Rat Mesenchymal Stem Cells Enhanced their Trans-differentiation Potential into
Cardiomyocytes. Paper presented at the 4th International Symposium-Cum-Training Course
on Molecular Medicine and Drug Research, held in Karachi, Pakistan (Jan. 7-10, 2013).

Kanwal Haneef, Nadia Naeem, Hana’a Iqbal, Nurul Kabir, Siddiqua Jamall, and Asmat
Salim. In vitro fusion and differentiation of bone marrow mesenchymal stem cells into
cardiomyocytes. Paper presented at the Symposium on Omics Research: Road Map for
Future held in Karachi, Pakistan (Feb. 22, 2012).
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Asmat Salim
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
Asmat Salim, Kanwal Haneef, Nadia Naeem, Nazia Ahmed, Hana’a Iqbal, Siddiqua Jamall
and Nurul Kabir. Preconditioned Bone Marrow Stem Cells and their Differentiation into
Cardiomyocytes. Paper presented at the 3rd International Symposium-cum-training course on
Molecular Medicine and Drug Research, held in Karachi, Pakistan (Jan. 3-6, 2011).

Asmat Salim, Kanwal Haneef, Nadia Naeem, Hana’a Iqbal, Nurul Kabir and Siddiqua
Jamall.
Transdifferentiation
Potential
of
Normal
and
Modified
Rat
Mesenchymal Stem Cells into Cardiomyocytes. Paper presented at the 3rd France-Pak binational Symposium held in Besançon, France (July 15-18, 2010).

Nadia Naeem, Kanwal Haneef, Siddiqua Jamall and Asmat Salim. Cardioprotective effect
of 5-azacytidine and zebularine on bone marrow mesenchymal stem cells. 47th Annual
Medical Symposium, Jinnah Postgraduate Medical Center, Karachi, Pakistan (Feb. 15-21,
2010).

Asmat Salim, Kanwal Haneef, Nadia Naeem, Hana’a Iqbal and Siddiqua Jamall.
Transdifferentiation
Potential
of
Normal
and
Engineered
Rat
Mesenchymal Stem Cells into Cardiomyocytes. Paper presented at the 2nd International
Symposium-cum-training course on Molecular Medicine and Drug Research held in
Karachi, Pakistan (Jan. 12-15, 2009).

Shabana U. Simjee, Asmat Salim and Raheela Khan. Suppression of Long-term Kindled
Seizures Induced alterations in hematopoietic functions in Bone Marrow Cells by Essential
Oil from Allium cepa. Poster presented at the 38th annual meeting of the Society for
Neuroscience in Washington, DC, USA (Nov. 15 to 19, 2008).

Asmat Salim, Kh Husnain Haider, Shujia Jiang, Shahida Shujaat and Muhammad Ashraf.
Transdifferentiation potential of young and aging bone marrow stem cells into
cardiomyocytes. Workshop on Human Stem Cell: Principles and Applications jointly
organized by COMSATS, Pakistan, UNESCO and National Research Center, Cairo Egypt
(Dec, 26-27, 2007).

Shujia Jiang, Husnain K Haider, Niagara Muhammad Idris, Rafeeq Ahmed, Asmat Salim
and Muhammad Ashraf (2007). Transplantation of mesenchymal stem cells constitutively
co-expressing Akt and Ang-1 leads to improved cardiac function in rodent model of acute
myocardial infarction (Supplemented to Circulation Vol 116, No 16, October 16, 2007).

Asmat Salim, Kh Husnain Haider, Shujia Jiang, Shahida Shujaat and Muhammad Ashraf.
Transdifferentiation Potential of Young and Aging Bone Marrow Stem Cells into
Cardiomyocytes. Paper presented at the 1st International Symposium-cum-training course on
Molecular Medicine and Drug Research held in Karachi, Pakistan (Jan. 16-30, 2007).

Husnain K Haidar, Shujia Jiang, Asmat Salim, Shahida Shujaat, Niagra M Idris, Ruowen
Ge, Uemura Royta, Ashraf Muhammad. Transplantation of mesenchymal stem cells
constitutively co-expressing Akt and Ang-1 leads to improved cardiac function in rodent
model of acute myocardial infarction (Supplemented to Circulation Vol. 112, No 17,
October 25, 2005).

Yigang Wang, Husnain kh Haidar, Nauman Ahmad, Jeff Brown, Royta Uemura, Meifeng
Xu, Sadia Sharif, Asmat Salim, Shahida Shujaat, Shujia Jiang, Ruowen Ge, Muhammad
Ashraf. Combining pharmacological mobilization of bone marrow stem cells with
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Asmat Salim
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intramyocardial injection of genetically modulated mesenchymal stem cells over expressing
VEGF for cardiac repair (Supplemented to Circulation Vol 112, No 17, October 25, 2005).

Asmat Salim and Zafar H. Zaidi. Prediction of stability factors at the domain interface of
Human γB-crystallin by Homology Modeling. Paper presented at the 7th International
Conference on Trends in Biochemistry and Molecular Biology organized by Pakistan
Society for Biochemistry and Molecular Biology in April 2003.

Asmat Salim and Zafar H. Zaidi. Prediction of structural changes in the two human γDcrystallin mutants caused by punctate, progressive juvenile-onset cataract and crystalline
aculeiform or frosted cataract. Poster presented at the 7th International Symposium of
Protein-Structure function relationship organized by HEJ Research Institute of Chemistry,
University of Karachi.

Asifa Bano, Asmat Salim, Atiya Abbasi and Zafar H. Zaidi (2002). Comparison of three
Classes (,  and ) of Snake Neurotoxins by homology Modeling. Poster presented at the
7th Eurasia Conference on Chemical Sciences organized by HEJ Research Institute of
Chemistry, University of Karachi.

Asmat Salim and Zafar H. Zaidi. Exploring the stability features of Human -crystallin by
homology modeling. Poster presented at the 7th Eurasia Conference on Chemical Sciences
organized by HEJ Research Institute of Chemistry, University of Karachi.

Asmat Salim and Zafar H. Zaidi. Homology Modeling of human gamma crystallins. Poster
presented at the 6th International Symposium of Protein-Structure function relationship
organized by HEJ Research Institute of Chemistry, University of Karachi

Asmat Salim, M. Kamran Azim and Zafar H. Zaidi. Homology Modeling of human alpha A
crystallin. Paper presented at the National Chemistry Symposium, University of Karachi

Sabira Naqvi, Asmat Salim and Zafar H. Zaidi. Poster presented on "Studies on water
insoluble proteins from human cataractous lenses" at the National Symposium on
Biochemistry and Molecular Biology in Lahore organized, April 1995 organized by the
Biochemical Society of Pakistan.

Asmat Salim, Riffat Haleem, Fouzia Murtaza, Ghazala Shameem, Tahira Perveen and
Darakhshan J. Haleem. Injected tryptophan increases brain but not plasma tryptophan level
more in stressed rats". Proceedings of the First International Conference of Pharmaceutical
Sciences.
HONOR(s) / AWARD(s)




Zaidi Abid Gold Medal for obtaining first class first position in M.Sc. program.
Merit certificates awarded from University of Karachi in 1990 and 1991.
Second Prize in poster competition at the 7th International Symposium on Protein Structure
Function relationship organized by H.E.J. Research Institute of Chemistry, University of
Karachi.
Travel grant awarded by Higher Education Commission, Pakistan to attend 38th annual
meeting of the Society for Neuroscience in Washington, DC, USA (Nov. 15 to 19, 2008)
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MEMBERSHIP OF SCIENTIFIC SOCIETIES


American Heart Association
International Society for Stem Cell Research (ISSCR)
PROFESSIONAL SKILLS






Protein purification techniques like conventional and automated (HPLC) chromatography,
Electrophoresis, Western Blotting, etc.
Spectrophotometeric techniques like UV, Visible, Infra Red spectroscopy etc.
Cell Biology: Tissue culture, Microscopy (Confocal, Phase-Contrast and Fluorescent),
Immuno-histochemistry, Flow Cytometry.
Molecular Biology: RT-PCR
Softwares involved in the Computer based structural prediction of simple and complex
proteins, e.g. Web Lab Viewer, Rasmol, Swiss Pdb Viewer etc.
Linux based softwares including MODELLER, WHATIF etc.
COURSES AND WORKSHOPS

Workshop on Human Stem Cell: Principles and Applications (Dec, 26-27, 2007) jointly
organized by COMSATS, Pakistan, UNESCO and National Research Center, Cairo Egypt.

S* Online Bioinformatics Course (17 Feb to 2 June 2003) organized by The S* Life Science
Informatics Alliance. The international course was run jointly by Karolinska Institutet,
National, University of Singapore (NUS), Stanford University, University of Western Cape's
South Africa National Bioinformatics Institute (SANBI), University of Sydney, Uppsala
University, Sweden, and University of California San Diego.
 Series of International workshops on Protein Structure Function Relationship from 19931997 organized by the Protein Chemistry Section at the HEJ Research Institute of
Chemistry, University of Karachi.
REFERENCES
Dr. Kh. Husnain Haider
Assistant Professor
Dept. of Pathology and Laboratory Medicine
University of Cincinnati,
Cincinnati, OH 45249
Email: [email protected]
Dr. Shamshad Zarina
Director
National Center for Proteomics
University of Karachi,
Karachi, Pakistan
Email: [email protected]
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