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Supplemental Information Iron-responsive chromatin remodeling and MAPK signaling enhance adhesion in Candida albicans RunningTitle: Iron-responsive chromatin remodeling in Candida albicans Sumant Puri1, 3, William K M Lai2, 3, Jason M Rizzo2, 3, Michael J Buck2* and Mira Edgerton1* 1 Department of Oral Biology, University at Buffalo, Buffalo, New York, 14214 USA 2 Department of Biochemistry and Center of Excellence in Bioinformatics and Life Sciences, University at Buffalo, Buffalo, New York, 14214 USA 3 These authors contributed equally to this work. * To whom the correspondence should be addressed: Mira Edgerton, 310 Foster Hall, Buffalo, NY 14214; Tel: (716)829-3067; E-mail: [email protected] or Michael J. Buck, 701 Ellicott St, Buffalo, NY 14203; Tel: (716)881-7569; E-mail: [email protected] Figure S1: MNase-seq profile correlations. Frequency histograms of Pearson correlation comparing non-overlapping 1 kb windows of MNase-seq data at 10 bp resolution are shown here. Comparisons were made between experimental replicates and across conditions. MNaseseq profile correlations demonstrate high reproducibility and sensitivity of assay. Table S2: Top-scoring genes potentially regulated by iron at the chromatin level. ORF CORR ∆OCC NAME orf19.6834.10 0.99 4668.71 TAR1 orf19.5514 0.90 114.96 - orf19.541 0.98 99.76 - orf19.5877 0.94 95.11 ATF1 orf19.6255 0.95 94.79 - orf19.7646 0.99 90.76 - orf19.7469 0.96 90.42 ARG1 orf19.6645 0.89 89.05 HMO1 orf19.1033 0.99 87.44 STR2 orf19.3307 0.99 86.20 - orf19.3749 0.97 84.93 IFC3 orf19.5513 0.91 84.31 - orf19.3175 0.93 83.90 - orf19.6319 0.96 80.92 - orf19.4225 0.98 80.14 LEU3 orf19.2587 0.97 79.34 HNM3 orf19.7497 0.93 78.45 - orf19.7468 0.94 77.83 - orf19.2618 0.99 77.58 MET2 orf19.3769 0.98 75.50 - orf19.7520 0.98 75.45 POT1 orf19.2107.1 0.97 75.30 STF2 orf19.7643 0.98 74.31 - orf19.7089 0.98 74.30 PMR1 orf19.6220 0.99 73.18 - DESCRIPTION Ortholog of S. cerevisiae Tar1p, encoded within the 25S rRNA gene on the opposite strand;transcription is positively regulated by Tbf1p Predicted ORF in Assemblies 19, 20 and 21; expression upregulated during growth in the mouse cecum Has domain(s) with predicted oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors activity Putative alcohol acetyltransferase; caspofungin repressed; expression depends on Tac1p Putative protein of unknown function; Hap43p-repressed gene Ortholog of C. glabrata CBS138 : CAGL0D03916g and C. parapsilosis CDC317 : CPAR2_200160 Argininosuccinate synthase; arginine biosynthesis; regulated by Gcn4p, Rim101p; induced by amino acid starvation (3-AT) and benomyl treatment; stationary phase enriched protein; repressed in alkalinizing medium; planktonic growth-induced Transcription factor that binds upstream of genes involved in hexose and ergosterol metabolism and in the cell cycle; activates pseudohyphal growth when expressed in S. cerevisiae; expression repressed in hyphae relative to yeast-form cells Ortholog(s) have cystathionine gamma-synthase activity, role in transsulfuration and cytosol, nucleus localization Ortholog(s) have N,N-dimethylaniline monooxygenase activity, role in protein folding and endoplasmic reticulum membrane, mitochondrion localization Oligopeptide transporter; transcriptionally induced upon phagocytosis by macrophage, by BSA or peptides; fluconazole-induced; upregulated by Rim101p at pH 8; virulence-group-correlated expression; no human/murine homolog; Hap43p-repressed Has domain(s) with predicted role in nuclear mRNA splicing, via spliceosome and spliceosomal complex localization Predicted ORF in Assemblies 19, 20 and 21; alkaline downregulated; repressed by nitric oxide; virulence-group-correlated expression Ortholog(s) have mRNA binding, ubiquitin-specific protease activity and role in protein deubiquitination, regulation of ER to Golgi vesicle-mediated transport, regulation of response to osmotic stress, ribophagy Zinc-finger protein; putative role in regulating branched-chain amino acid biosynthesis genes; alkaline upregulated; transcriptionally activated by Mnl1p under weak acid stress; required for yeast cell adherence to silicone substrate Putative transporter; Hap43p-repressed gene; flucytosine repressed; possibly an essential gene, disruptants not obtained by UAU1 method Has domain(s) with predicted ubiquitin protein ligase binding activity, role in ubiquitin-dependent protein catabolic process and cullin-RING ubiquitin ligase complex localization Putative transcriptional activator; expression upregulated during growth in the mouse cecum Homoserine acetyltransferase; Hap43p-, Gcn4p-regulated; macrophage/pseudohyphal-repressed; not highly biofilm induced, in contrast to many sulfur amino acid metabolic genes; no human or murine homolog; virulence-group-correlated expression Ortholog(s) have zinc ion transmembrane transporter activity, role in zinc ion transport and endoplasmic reticulum localization Putative peroxisomal 3-oxoacyl CoA thiolase; transcription is regulated by Nrg1p and Mig1p; farnesol regulated; Hap43p-repressed gene Protein involved in ATP biosynthesis; decreased expression in hyphae vs yeastform cells; downregulated by Efg1p, Hap43p; transcription upregulated in clinical isolates from HIV+ patients with oral candidiasis; biofilm-induced Ortholog(s) have 4-hydroxybenzoate octaprenyltransferase activity, antioxidant activity and role in polyprenol biosynthetic process, ubiquinone biosynthetic process Putative secretory pathway P-type Ca2+/Mn2+-ATPase; required for protein glycosylation and cell wall maintenance; required for hyphal tip oscillation in semisolid substrate; putative ortholog of S. cerevisiae PMR1 Ortholog(s) have role in positive regulation of transcription elongation from RNA polymerase II promoter and CCR4-NOT core complex localization Putative dihydrolipoamide S-succinyltransferase; transcriptionally regulated by iron; expression greater in high iron; Hap43p-repressed gene T-complex protein 1, epsilon subunit; protein present in exponential and stationary growth phase yeast cultures Protein similar to S. cerevisiae Eci1p, which is involved in fatty acid oxidation; transposon mutation affects filamentous growth; expression is regulated upon white-opaque switching orf19.6126 0.85 73.01 KGD2 orf19.2288 0.98 71.89 CCT5 orf19.6445 1.00 71.60 ECI1 orf19.5293 0.98 71.52 - orf19.5294 0.94 71.11 PDB1 orf19.3250 0.96 70.40 - orf19.2138 0.99 70.37 ILS1 orf19.1517 0.73 70.31 ARO3 orf19.6507 0.97 69.36 - orf19.6382 0.98 68.99 - orf19.6508 0.97 68.89 - orf19.2891 0.93 68.72 - orf19.490 0.94 68.64 - Ortholog(s) have NatA complex, cytosol, cytosolic ribosome, nucleus localization orf19.2038 0.97 68.42 - - orf19.6701 0.97 68.41 - orf19.6377 0.99 68.25 - orf19.597 0.98 67.89 - orf19.1461 0.98 67.83 - orf19.6500 0.97 67.58 ECM42 orf19.2876 0.97 66.87 CBF1 orf19.6391 0.94 66.76 - orf19.3827 0.96 66.45 - orf19.3097 0.98 65.89 PDA1 orf19.7096 0.97 65.76 - Ortholog(s) have fungal-type vacuole membrane, mitochondrion localization Ortholog of C. parapsilosis CDC317 : CPAR2_402940 Putative pyruvate dehydrogenase; fluconazole-induced; protein level decreases in stationary phase cultures Ortholog(s) have RNA binding activity, role in nuclear mRNA cis splicing, via spliceosome and U1 snRNP, U2-type prespliceosome localization Putative isoleucyl-tRNA synthetase, the target of drugs including the cyclic beta-amino acid icofungipen/PLD-118/BAY-10-8888 and mupirocin; protein present in exponential and stationary growth phase yeast cultures 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase; aromatic amino acid biosynthesis; GCN-regulated; feedback-inhibited by phenylalanine if expressed in S. cerevisiae; Aro3p and Aro4p catalyze same reaction; decreased in stationary phase Stationary phase enriched protein Putative MRP/CFTR-subfamily ABC transporter; member of multidrug resistanceassociated protein (MRP) subfamily of ABC family; similar to S. cerevisiae Bpt1p Ortholog(s) have protein transporter activity, role in Golgi to plasma membrane transport, intracellular protein transport and vesicle coat localization Ortholog(s) have role in cellular response to oxidative stress, misfolded or incompletely synthesized protein catabolic process, protein import into peroxisome matrix and mitochondrial inner membrane localization Protein with similarity to amino acid-tRNA ligase; stationary phase enriched protein; GlcNAc-induced protein Ortholog(s) have protein C-terminal leucine carboxyl O-methyltransferase activity, role in C-terminal protein methylation, cellular protein complex assembly and mitochondrion localization Predicted ORF in Assemblies 19, 20 and 21; Gcn4p-regulated Predicted ORF in Assemblies 19, 20 and 21; possibly transcriptionally regulated upon hyphal formation Putative ornithine acetyltransferase; fungal-specific (no human or murine homolog); Gcn2p-, Gcn4p-regulated; clade-specific gene expression; possibly an essential gene, disruptants not obtained by UAU1 method Transcription factor that binds upstream of ribosomal protein genes and the rDNA locus, with Tbf1p; also regulates sulfur starvation-response, respiratory, and glycolytic genes; does not bind to centromeres as does S. cerevisiae Cbf1p Increased transcription is observed in an azole-resistant strain that overexpresses MDR1 Ortholog(s) have metalloendopeptidase activity, role in misfolded or incompletely synthesized protein catabolic process and mitochondrial inner membrane localization Putative pyruvate dehydrogenase alpha chain; fluconazole-induced; protein present in exponential and stationary growth phase yeast cultures; Hap43pinduced gene orf19.715 0.98 65.59 - Hap43p-induced gene orf19.2132 0.99 65.46 - Ortholog of C. parapsilosis CDC317 : CPAR2_213740 orf19.1736 0.99 65.40 - Predicted ORF in Assemblies 19, 20 and 21; transcription detected in highresolution tiling array experiments orf19.4086 0.99 64.81 - Ortholog(s) have cytoplasm localization orf19.7341.1 0.97 64.79 - - orf19.407 1.00 64.16 GCD6 Putative catalytic epsilon subunit of the translation initiation factor eIF2B, based on similarity to S. cerevisiae Gcd6p; genes encoding translation factors are downregulated upon phagocytosis by murine macrophage orf19.7130 0.96 63.88 - Hap43p-repressed gene orf19.7328 0.98 63.30 - Gene transcription regulated by Mig1p and Tup1p orf19.3163 0.99 63.27 - - orf19.6607 0.98 63.13 - Ortholog of C. parapsilosis CDC317 : CPAR2_201110 orf19.6016 0.98 63.13 - orf19.3954 0.99 63.12 - orf19.2028 1.00 62.94 MXR1 orf19.6702 0.98 62.84 DED81 orf19.3915 0.98 62.78 - orf19.5444 0.98 62.58 TIM44 orf19.5131 0.98 62.54 - orf19.2154 0.99 62.51 HXK1 orf19.6211 0.98 62.36 - orf19.6387 0.89 62.30 HSP104 orf19.496 0.99 62.16 - orf19.2473 0.99 62.03 - orf19.498 0.99 61.73 - orf19.5672 0.99 61.71 MEP2 orf19.7272 1.00 61.58 - orf19.5645 0.98 61.58 MET15 orf19.2287 0.98 61.50 RPA12 Putative DNA-directed RNA polymerase I; induced upon adherence to polystyrene orf19.7156 0.94 61.48 FAA2-3 Predicted acyl CoA synthetase orf19.5475 0.98 61.37 - orf19.1750 0.98 61.23 SLR1 orf19.5809 0.99 61.03 - orf19.1460 0.96 60.80 - orf19.3264 0.98 60.68 CCE1 orf19.2529.1 0.96 60.36 - orf19.7498 0.76 60.35 LEU1 Ortholog(s) have GTPase activity, calcium ion binding activity, role in mitochondrion inheritance, phospholipid homeostasis, regulation of mitochondrion organization and ERMES complex, cytosol, nuclear envelope localization Ortholog(s) have phosphatidylserine decarboxylase activity and role in phosphatidylcholine biosynthetic process, phosphatidylethanolamine biosynthetic process, regulation of cell cycle cytokinesis, regulation of cell shape Putative methionine sulfoxide reductase; flucytosine induced; Plc1p-regulated; upregulated in the presence of human neutrophils; macrophage regulated (gene induced, protein decreased); possibly adherence-induced Putative tRNA-Asn synthetase; genes encoding ribosomal subunits, translation factors, tRNA synthetases are downregulated upon phagocytosis by murine macrophage; protein enriched in stationary phase yeast cultures Putative metallodipeptidase; protein present in exponential and stationary growth phase yeast cultures; Hog1p-induced; Hap43p-repressed; sumoylation target Protein involved in transport across membranes Hap43p-repressed gene N-acetylglucosamine (GlcNAc) kinase; involved in GlcNAc utilization; required for wild-type hyphal growth and mouse virulence; gene expression is GlcNAcinduced; induced in response to alpha pheromone in SpiderM medium Ortholog of C. parapsilosis CDC317 : CPAR2_209000 Heat-shock protein; functional homolog of S. cerevisiae Hsp104p; chaperone and prion propagation activity in S. cerevisiae; guanidine-insensitive; heat shock/stress induced; downregulated in farnesol-treated biofilm; sumoylation target Ortholog(s) have DNA-dependent ATPase activity, dinucleotide insertion or deletion binding, guanine/thymine mispair binding activity, role in mismatch repair, mitochondrial DNA repair and mitochondrion localization Putative RSC chromatin remodeling complex component; decreased transcription is observed upon fluphenazine treatment or in an azole-resistant strain that overexpresses CDR1 and CDR2 Putative mitochondrial ribosomal component of the small subunit; possibly an essential gene, disruptants not obtained by UAU1 method Ammonium permease and regulator of nitrogen starvation-induced filamentation; 11 predicted transmembrane regions; in low nitrogen cytoplasmic C-terminus activates Ras/cAMP and MAPK signal transduction pathways to induce filamentation Predicted ORF in Assemblies 19, 20 and 21; transcription detected in highresolution tiling array experiments O-acetylhomoserine O-acetylserine sulfhydrylase; involved in sulfur amino acid biosynthesis; immunogenic; Hog1p, biofilm, possibly adherence-induced; brown colony color of homozygous mutant in Pb(2+) medium is visual selection marker Sef1p-, Sfu1p-, and Hap43p-regulated gene Protein similar to mammalian SR-like RNA splicing factor; gene has intron; significant changes made to ORF with coordinate updates (see Locus History) Putative arylformamidase, enzyme of the NAD biosynthesis pathway; Gcn4pregulated Putative glutamine-dependent NAD synthetase, involved in NAD salvage pathway Putative Holliday junction resolving enzyme; similar to S. cerevisiae Cce1p 3-isopropylmalate dehydratase; antigenic in humans; decreased expression in hyphae vs yeast cells; alkaline downregulated; upregulated by human whole blood or PMN cells; regulated by Sef1p-, Sfu1p-, and Hap43p Putative glutaredoxin; described as a glutathione reductase; upregulated in the presence of human neutrophils, and upon benomyl treatment; alkaline downregulated; regulated by Gcn2p and Gcn4p; required for virulence in mouse model Protein of unknown function, transcription is upregulated in an RHE model of oral candidiasis; Hap43p-repressed gene orf19.6059 0.99 60.23 TTR1 orf19.1414 0.88 60.22 - orf19.7662 0.99 60.18 - Ortholog of C. parapsilosis CDC317 : CPAR2_200060 orf19.3029 0.86 59.82 - Ortholog(s) have 3-hydroxyisobutyryl-CoA hydrolase activity and mitochondrion localization orf19.7529 0.96 59.78 EPL1 Subunit of the NuA4 histone acetyltransferase complex orf19.3881 0.99 59.67 - Ortholog of C. parapsilosis CDC317 : CPAR2_204900 orf19.5805 0.93 59.66 DLD1 orf19.1052 0.93 59.57 - orf19.238 0.58 59.36 CCP1 orf19.522 0.93 59.32 - orf19.2983 0.99 59.29 CDC73 orf19.7270 0.91 59.24 - orf19.6602 0.96 58.98 - orf19.1451 0.98 58.93 SRB9 orf19.1450 0.98 58.73 - orf19.3815 0.95 58.67 - orf19.7281 0.93 58.58 PDK2 orf19.2137 0.98 58.56 - orf19.6444 1.00 58.49 - orf19.5965 0.96 58.40 - orf19.6561 0.95 58.33 LAT1 orf19.6259 0.98 58.29 - orf19.2529 0.97 58.17 - orf19.7047 0.91 58.13 RTF1 orf19.6171 0.99 57.99 - orf19.6988 0.74 57.91 OST1 Putative oligosaccharyltransferase orf19.497 0.99 57.89 EAF7 Subunit of the NuA4 histone acetyltransferase complex orf19.2158 0.99 57.84 NAG3 Putative transporter of the major facilitator superfamily (MFS); similar to Nag4p; required for wild-type mouse virulence and cycloheximide resistance; in gene cluster that includes genes encoding enzymes of GlcNAc catabolism orf19.2836 0.95 57.73 - Protein with similarity to carbonic anhydrases orf19.4959 0.84 57.66 - Ortholog of C. parapsilosis CDC317 : CPAR2_202690 orf19.1854 0.94 57.65 HHF22 Putative D-lactate dehydrogenase; transcription is specific to white cell type; shows colony morphology-related gene regulation by Ssn6p; transcription is upregulated in both intermediate and mature biofilms; Hap43p-repressed gene Predicted histone H2B; Hap43p-induced gene Similar to cytochrome-c peroxidase N terminus; negatively regulated by Rim101p or alkaline pH; transcription induced by interaction with macrophage or low iron; oxygen-induced activity; regulated by Sef1p-, Sfu1p-, and Hap43p Has domain(s) with predicted ATP binding, ATP-dependent peptidase activity, serine-type endopeptidase activity and role in proteolysis Protein similar to S. cerevisiae Cdc73p, which is a transcription elongation factor; cdc73 kap114 double transposon mutation affects filamentous growth Ortholog of C. parapsilosis CDC317 : CPAR2_808740 Ortholog(s) have role in aerobic respiration, ubiquinone biosynthetic process and mitochondrial inner membrane localization Protein with similarity to S. cerevisiae Srb9p transcription mediator; transposon mutation affects filamentous growth; suppresses S. cerevisiae diploid filamentous (flo8, ste7, ste12, tec1) or haploid invasive (flo8) mutant growth defects Has domain(s) with predicted ubiquitin thiolesterase activity and role in ubiquitindependent protein catabolic process Putative pyruvate dehydrogenase kinase; mutation confers hypersensitivity to amphotericin B Ortholog(s) have mitochondrion localization Ortholog(s) have role in cellular response to amino acid starvation, meiosis, regulation of TOR signaling cascade and Seh1-associated complex, cytosol, extrinsic to fungal-type vacuolar membrane localization Ortholog(s) have ubiquitin-ubiquitin ligase activity, role in ER-associated protein catabolic process, protein ubiquitination, response to stress and cytoplasm, nucleus localization Putative dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex; sumoylation target Ortholog(s) have role in exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA and LSU-rRNA), more Predicted ORF in Assemblies 19, 20 and 21; shares similarity with human Pig-H, which is involved in glycosylphosphatidylinositol assembly Putative RNA polymerase II-associated Paf1 complex subunit; induced during the mating process Ortholog of C. parapsilosis CDC317 : CPAR2_808010 Putative histone H4; regulated by Efg1p; flucytosine, fluconazole-induced; amphotericin B, caspofungin repressed; shows colony morphology-related gene regulation by Ssn6p; Hap43p-induced Putative gamma-glutamyl kinase; transcription is regulated by Nrg1p; regulated by Gcn2p and Gcn4p; Hap43p-repressed gene; biofilm- and planktonic growthinduced gene Protein similar to S. cerevisiae Prp8p; transcription is repressed in response to alpha pheromone in SpiderM medium Chaperone for Crk1p; interacts with Crk1p kinase domain and with Sti1p; putative phosphorylation site at Ser14; functional homolog of S. cerevisiae Cdc37p; likely to be essential for growth; regulated by Gcn2p and Gcn4p orf19.7638 0.98 57.61 PRO1 orf19.6442 0.98 57.53 PRP8 orf19.5531 0.96 57.35 CDC37 orf19.3136 0.98 57.31 - orf19.3265 0.97 57.12 TRM1 orf19.6448 0.99 57.04 - orf19.7648 0.97 56.95 - orf19.1619 0.98 56.68 - orf19.6641 0.58 56.58 - - orf19.6438 0.91 56.19 - Ortholog(s) have serine C-palmitoyltransferase activity, role in sphingolipid biosynthetic process and SPOTS complex, microsome localization orf19.4418.1 1.00 56.07 - Ortholog of C. parapsilosis CDC317 : CPAR2_501415 orf19.7341 0.99 56.07 - Late-stage biofilm-induced gene; ketoconazole-repressed orf19.1012 1.00 55.69 - orf19.7637 0.98 55.66 YHB4 orf19.5719 0.99 55.60 - orf19.6440 0.98 55.58 - orf19.7105 0.99 55.58 FAR1 orf19.3748 0.96 55.56 - orf19.6414 0.94 55.50 - orf19.2593 0.84 55.35 BIO2 orf19.2645 0.97 55.33 - - orf19.1219 0.97 55.15 - - orf19.1195 0.98 55.09 - orf19.6809 0.98 55.04 - orf19.3817 0.98 55.03 - Ortholog(s) have cytoplasm, nucleolus localization orf19.7261 0.99 54.92 - Putative Rab GDP-dissociation inhibitor; GlcNAc-induced protein orf19.6596 0.97 54.87 - Putative esterase; possibly transcriptionally regulated by Tac1p; transcriptionally activated by Mnl1p under weak acid stress; protein present in exponential and stationary growth phase yeast cultures orf19.7109 0.99 54.78 - Ortholog(s) have role in meiosis and cytosol, nucleus localization orf19.1519 0.96 54.77 - Hap43p-repressed gene Has domain(s) with predicted role in response to stress Putative N2,N2-dimethylguanine tRNA methyltransferase; induced upon adherence to polystyrene Ortholog of C. parapsilosis CDC317 : CPAR2_206180 Has domain(s) with predicted antiporter activity, drug transmembrane transporter activity, role in drug transmembrane transport and membrane localization Putative kinase subunit of RNA polymerase II carboxy-terminal domain kinase I; possibly an essential gene, disruptants not obtained by UAU1 method Ortholog(s) have clathrin binding activity, role in Golgi to vacuole transport, endosome transport and AP-1 adaptor complex, cytosol, endosome, nucleus localization Protein related to flavohemoglobins; not required for wild-type nitric oxide resistance; has predicted globin, FAD-binding, and NAD(P)-binding domains but lacks some conserved residues of flavohemoglobins; Hap43p-repressed gene Predicted ORF from Assembly 19; removed from Assembly 20; subsequently reinstated in Assembly 21and merged with orf19.1020 based on comparative genome analysis Ortholog(s) have ubiquitin-protein ligase activity, role in ubiquitin-dependent protein catabolic process and endoplasmic reticulum membrane, nuclear inner membrane localization Protein involved in regulation of pheromone-mediated mating; repressed by A1p and Alpha2p in white-phase cells; null mutant shows no pheromone response in opaque cells; overexpression causes enhanced pheromone response and cell cycle arrest Biofilm-induced gene; orf19.3747 and orf19.3748 are short, overlapping ORFs in intergenic region between two oligopeptide transporter-encoding genes Putative biotin synthase; transcriptionally upregulated in high iron; transcription downregulated by treatment with ciclopirox olamine; upregulated in clinical isolates from HIV+ patients with oral candidiasis; Hap43p-repressed Ortholog(s) have metalloendopeptidase activity, role in cellular iron ion homeostasis, protein processing involved in protein targeting to mitochondrion and mitochondrion localization Putative phosphomutase-like protein; protein present in exponential and stationary growth phase yeast cultures; Hap43p-repressed gene; planktonic growth-induced gene orf19.1178 0.99 54.76 - Putative transcription factor with bZIP DNA-binding motif; Hap43p-repressed gene orf19.301 0.98 54.72 PGA18 orf19.4220 0.99 54.59 - orf19.5647 0.98 54.56 SUB2 orf19.596.2 0.99 54.54 DAD4 orf19.392 0.98 54.49 - orf19.2832 0.94 54.48 INN1 orf19.3818 0.98 54.41 GOA1 orf19.2485 0.98 54.36 - orf19.5865 0.97 54.34 - orf19.3054 0.99 54.28 RPN3 orf19.913.2 0.94 54.25 - Hap43p-repressed gene orf19.7010 0.98 54.19 - Ortholog(s) have cytosol, ubiquitin ligase complex localization orf19.1220 0.97 54.15 RVS167 orf19.5446 0.97 54.06 - orf19.6499 0.97 54.03 - orf19.637 0.48 54.02 SDH2 orf19.1482 0.97 53.99 HSK3 orf19.4718 0.98 53.99 TRP5 orf19.583 0.99 53.95 - orf19.2803 0.91 53.93 HEM13 orf19.4099 0.95 53.92 ECM17 orf19.4828 0.98 53.87 - Hap43p-repressed gene; biofilm-induced orf19.4081 0.95 53.71 - - orf19.2721 0.99 53.69 - orf19.669 0.99 53.61 PRM1 orf19.216.1 0.98 53.51 - Putative GPI-anchored protein; regulated by Nrg1p, Tup1p Predicted ORF in Assemblies 19, 20 and 21; regulated by Gcn4p; repressed in response to amino acid starvation (3-aminotriazole treatment) Putative TREX complex component with a predicted role in nuclear mRNA export; transcription is regulated by Mig1p; Hap43p-induced gene Subunit of the Dam1 (DASH) complex, which acts in chromosome segregation by coupling kinetochores to spindle microtubules Ortholog(s) have role in mitochondrial respiratory chain complex III assembly and integral to mitochondrial membrane localization Protein with similarity to S. cerevisiae Inn1p, which is an essential protein of the contractile actomyosin ring required for ingression of the plasma membrane into the bud neck during cytokinesis; contains a C2 membrane targeting domain Protein required for respiratory growth, resistance to oxidants, chlamydospore formation, hyphal growthunder some conditions, and virulence; relocalizes from the cytoplasm to the mitochondrion during oxidative or osmotic stress Ortholog(s) have role in chromosome segregation, nuclear pore complex assembly, protein targeting to membrane and cytoplasm, nuclear pore localization Ortholog(s) have RNA-dependent ATPase activity, role in generation of catalytic spliceosome for first transesterification step and U2-type catalytic step 1 spliceosome localization Putative non-ATPase regulatory subunit of the 26S proteasome lid; amphotericin B repressed; oxidative stress-induced via Cap1p SH3-domain- and BAR domain-containing protein involved in endocytosis; null mutant exhibits defects in hyphal growth, virulence, cell wall integrity, and actin patch localization; cosediments with phosphorylated Myo5p Putative protein of unknown function, transcription is upregulated in clinical isolates from HIV+ patients with oral candidiasis; regulated by Ssn6p Has domain(s) with predicted DNA binding, DNA-directed RNA polymerase activity and role in transcription, DNA-dependent Succinate dehydrogenase, Fe-S subunit; localizes to surface of yeast cells, but not hyphae; transcriptionally regulated by iron; expression greater in high iron; repressed by nitric oxide, Hap43p; induced during log phase aerobic growth Subunit of the Dam1 (DASH) complex, which acts in chromosome segregation by coupling kinetochores to spindle microtubules; expression regulated during planktonic growth Predicted tryptophan synthase; identified in detergent-resistant membrane fraction (possible lipid raft component); predicted N-terminal acetylation; Gcn4p-regulated; S. cerevisiae ortholog is Gcn4p regulated; upregulated in biofilm; Putative indoleamine 2,3-dioxygenase (IDO); IDO is the rate-limiting enzyme of tryptophan catabolism via kynurenine pathway; enzyme inhibition by 1-methylD,L-tryptophan causes increased hyphal growth; expressed in yeast cells and hyphae Coproporphyrinogen III oxidase; antigenic; localizes to yeast cell surface, not hyphae; iron-regulated expression; macrophage-downregulated; not Rfg1p regulated, farnesol-induced; possibly essential; Hap43p-repressed gene; biofilminduced Enzyme of sulfur amino acid biosynthesis; putative role in regulation of cell wall biogenesis; upregulated in biofilm; possibly adherence-induced; regulated by Tsa1p/Tsa1Bp in H2O2 stress; Gcn4p-regulated; Tbf1p-activated; Hap43prepressed Ortholog(s) have role in meiosis, regulation of mitotic cell cycle, regulation of translation and cytosol, nucleus localization Putative membrane protein with a predicted role in membrane fusion during mating; Hap43p-repressed gene; protein induced during the mating process Ortholog of C. parapsilosis CDC317 : CPAR2_806510 orf19.5835 0.91 53.40 - orf19.7512 0.97 53.39 - orf19.5646 0.95 53.24 - orf19.5033 0.98 53.22 - orf19.5720 0.99 53.19 - orf19.6843 0.98 53.19 - orf19.6699 0.99 53.17 - orf19.3593 0.99 53.11 RPT6 orf19.6127 0.99 52.98 LPD1 orf19.3265.1 0.97 52.97 - Ortholog(s) have role in rRNA processing, ribosomal small subunit assembly and CURI complex, UTP-C complex, cytosol localization Has domain(s) with predicted electron carrier activity, heme binding, oxidoreductase activity, acting on paired donors and with incorporation or reduction of molecular oxygen, more Ortholog of S. cerevisiae : YPL034W, C. glabrata CBS138 : CAGL0L06996g and C. parapsilosis CDC317 : CPAR2_402170 Ortholog(s) have Atg8 ligase activity, protein tag activity Predicted membrane transporter, member of the monocarboxylate porter (MCP) family, major facilitator superfamily (MFS); ketoconazole or caspofungin repressed Ortholog(s) have role in CenH3-containing nucleosome assembly at centromere, chromatin silencing at centromere, mitotic sister chromatid segregation and nucleus localization Ortholog(s) have histidinol-phosphatase activity, role in histidine biosynthetic process and cytosol, nucleus localization Putative ATPase of the 19S regulatory particle of the 26S proteasome; transcription is regulated by Mig1p; regulated by Gcn2p and Gcn4p Putative dihydrolipoamide dehydrogenase; soluble in hyphae; antigenic in human oral infection and murine systemic infection; macrophage-induced protein; protein present in exponential and stationary phase yeast cultures; Hap43p-repressed Ortholog of C. parapsilosis CDC317 : CPAR2_800920 SUMO, small ubiquitin-like protein; Smt3p-conjugated proteins localize to septation site and mother side of bud neck; C. albicans septins appear not to be Smt3p-modified, in contrast to S. cerevisiae septins Homoaconitase; regulated by Gcn4p, Gcn2p; induced in response to amino acid starvation (3-AT treatment); upregulated in the presence of human whole blood or polymorphonuclear (PMN) cells; Hap43p-repressed gene orf19.670 0.99 52.91 SMT3 orf19.3846 0.87 52.81 LYS4 orf19.258 0.99 52.80 - orf19.5985 0.99 52.75 - orf19.6447 0.98 52.75 ARF1 orf19.2474 0.99 52.71 PRC3 orf19.1853 0.92 52.68 HHT2 Putative histone H3; farnesol regulated; Hap43p-induced gene orf19.2615 0.99 52.64 MDL1 Putative mitochondrial, half-size MDR-subfamily ABC transporter orf19.6422 0.97 52.63 SSY5 Ortholog(s) have serine-type endopeptidase activity, role in protein processing, response to amino acid stimulus and extrinsic to plasma membrane localization orf19.7534 0.91 52.58 MIS12 Mitochondrial C1-tetrahydrofolate synthase precursor orf19.4674.1 0.23 52.52 CRD2 orf19.7604 0.98 52.52 - orf19.3802 0.94 52.41 PMT6 orf19.670.2 0.93 52.40 - orf19.5563 0.98 52.40 RNH1 orf19.5076 0.99 52.37 PFY1 orf19.3314 0.91 52.32 - orf19.2081 0.99 52.26 POM152 Ortholog(s) have tubulin binding activity, role in tubulin complex assembly and nucleus, polysome, prefoldin complex localization ADP-ribosylation factor, probable GTPase involved in intracellular transport; one of several C. albicans ADP-ribosylation factors; N-myristoylprotein; substrate of Nmt1p Putative carboxypeptidase Y precursor; transcription is regulated by Nrg1p and Mig1p; regulated by Gcn2p and Gcn4p Metallothionein; role in adaptation to growth in high copper; basal transcription is cadmium-repressed; regulated by Ssn6p; complements copper sensitivity of an S. cerevisiae cup1 null mutant; regulated by Sef1p-, Sfu1p-, and Hap43p Ortholog(s) have Rab guanyl-nucleotide exchange factor activity and role in early endosome to Golgi transport, intra-Golgi vesicle-mediated transport, regulation of Rab GTPase activity Protein mannosyltransferase, required for virulence in mice and for adhesion to endothelium; role in hyphal growth signaling and hygromycin B sensitivity; not a major contributor to overall cellular PMT activity; Hap43p-repressed gene Hap43p-repressed gene; hypoxia downregulated, ketoconazole induced; latestage biofilm-induced Putative Ribonuclease H (RNAse H); hyphal-induced; flucytosine induced; similar to orf19.5564 (see Locus History); possibly an essential gene, disruptants not obtained by UAU1 method Profilin, functional homolog of S. cerevisiae Pfy1p; hyphal-induced; macrophage/pseudohyphal-induced; regulated by Nrg1p, Tup1p; gene lacks intron (unlike S. cerevisiae PFY1); complements growth of S. cerevisiae srv2 mutant; nonessential Ortholog(s) have role in ER to Golgi vesicle-mediated transport and TRAPP complex, cytosol, nucleus localization Putative nuclear pore membrane glycoprotein Protein with strong similarity to both S. cerevisiae Ntg1p and Ntg2p DNA repair glycosylases; transcriptionally regulated by iron; expression greater in high iron; Hap43p-repressed gene orf19.5098 0.96 52.25 NTG1 orf19.6136 0.99 52.13 - orf19.6636 0.96 52.11 - orf19.6779 0.96 52.04 PRO2 orf19.1167 0.97 51.97 - orf19.2157 0.99 51.95 DAC1 orf19.5606 0.99 51.95 - orf19.6671 0.96 51.93 LAP4 orf19.314 1.00 51.85 - orf19.3170 1.00 51.85 - orf19.2959.1 0.99 51.82 - orf19.6392 0.98 51.68 - orf19.3845 0.99 51.61 FGR3 Protein lacking an ortholog in S. cerevisiae; transposon mutation affects filamentous growth orf19.7513 0.99 51.52 ALK2 N-Alkane inducible cytochrome P450 orf19.3886 1.00 51.49 - orf19.6327 0.99 51.44 HET1 orf19.1961 0.98 51.30 - orf19.6956 0.97 51.27 DAL9 orf19.1083 0.99 51.15 - Putative protein of unknown function; macrophage-induced gene orf19.1179 0.99 51.06 - Transcriptionally regulated by iron; expression greater in high iron; possibly subject to Kex2p processing; Hap43p-repressed gene orf19.2633.1 0.91 50.99 - - orf19.5440 0.93 50.94 RPT2 orf19.6898 0.89 50.91 - orf19.6413 0.98 50.90 - - orf19.7337 0.95 50.68 - Ortholog(s) have cytosol, fungal-type vacuole membrane, nucleus localization orf19.1352 0.98 50.56 TIM22 orf19.317 0.98 50.55 - orf19.5139 0.99 50.46 - orf19.4000 0.98 50.43 GRF10 orf19.2075 0.99 50.38 DFG5 orf19.6423 0.97 50.38 - orf19.3161 0.99 50.37 - orf19.6369 0.99 50.34 RIO2 orf19.4439 0.99 50.34 - Predicted ORF in Assemblies 19, 20 and 21; repressed by nitric oxide Ortholog of S. cerevisiae : YPR084W, C. glabrata CBS138 : CAGL0K07898g and C. parapsilosis CDC317 : CPAR2_702430 Putative gamma-glutamyl phosphate reductase with a predicted role in proline biosynthesis; regulated by Gcn2p and Gcn4p Ortholog(s) have sulfonate dioxygenase activity and role in sulfur compound catabolic process N-acetylglucosamine-6-phosphate (GlcNAcP) deacetylase; enzyme of Nacetylglucosamine utilization; required for wild-type hyphal growth and virulence in mouse systemic infection; gene and protein are GlcNAc-induced Protein similar to aminopeptidase I; mutation confers hypersensitivity to amphotericin B; transcriptionally regulated during macrophage response; biofilminduced Ortholog(s) have Sin3-type complex localization Ortholog(s) have role in formation of translation preinitiation complex and cytoplasm, cytosolic ribosome localization Gene induced by hypoxia and ketoconazole; oral infection upregulated; mutants have reduced capacity to damage oral epithelial cells Ortholog of C. parapsilosis CDC317 : CPAR2_204450 Putative sphingolipid transfer protein involved in localization of glucosylceramide, which is important for virulence Planktonic growth-induced gene Putative allantoate permease; fungal-specific (no human or murine homolog) Putative ATPase of the 19S regulatory particle of the 26S proteasome; oxidative stress-induced via Cap1p Predicted ORF in Assemblies 19, 20 and 21; increased transcription is observed upon benomyl treatment or in an azole-resistant strain that overexpresses MDR1 Mitochondrial inner membrane protein with a predicted role in protein import; Hap43p-repressed gene; late-stage biofilm-induced gene Ortholog(s) have inosine nucleosidase activity, nicotinamide riboside hydrolase activity, purine-nucleoside phosphorylase activity Putative homeodomain transcription factor; null mutant exhibits adenine auxotrophy N-linked mannoprotein of cell wall and membrane; possible signal transducer; role in hyphal growth and HWP1 induction in alkaline pH; GPI modification predicted; dfg5 dcw1 double mutant is inviable; caspofungin-induced, fungal-specific Ortholog(s) have fructose-2,6-bisphosphate 2-phosphatase activity and role in glucose metabolic process Ortholog(s) have ATPase activator activity, RNA binding activity Putative serine kinase with a predicted role in the processing of the 20S pre-rRNA into mature 18S rRNA; null mutants are hypersensitive to caspofungin - Putative histidinol-phosphate aminotransferase; Gcn4p-regulated; protein present in exponential and stationary growth phase yeast cultures Component of 20S pre-rRNA processing unit; decreased expression in response to prostaglandins orf19.4177 0.92 50.28 HIS5 orf19.6417 0.98 50.25 TSR1 orf19.6443 0.94 50.21 - orf19.5005 0.90 50.14 OSM2 orf19.6509 1.00 50.09 - orf19.5129 0.97 50.08 - orf19.6744 0.98 50.04 - orf19.4433 0.97 50.04 CPH1 orf19.6047 0.95 50.02 TUF1 orf19.2341 0.99 49.98 HNT1 orf19.1061 0.71 49.86 HHT21 orf19.5337 0.99 49.83 UBC15 Putative E2 ubiquitin-conjugating enzyme orf19.2108 0.97 49.74 SOD6 Copper- and zinc-containing superoxide dismutase; expression regulated during planktonic growth; member of a gene family that includes SOD1, SOD4, SOD5, and SOD6; gene may contain an intron; Hap43p-repressed; biofilm-induced orf19.6739 0.99 49.74 - Ortholog(s) have phosphoglucomutase activity and cytosol, nucleus localization orf19.731 0.98 49.62 EMP46 orf19.6854 0.97 49.59 ATP1 orf19.5443 0.98 49.58 BNA4 orf19.3199 0.95 49.53 PIKA orf19.5493 0.89 49.46 GSP1 orf19.2687.1 0.99 49.41 - orf19.5897 0.99 49.38 - orf19.7588 0.97 49.36 - orf19.327 0.97 49.34 HTA3 orf19.4372 0.88 49.32 - orf19.5132 0.95 48.92 - orf19.1763 0.99 48.92 IFR1 orf19.3655 0.48 48.90 - orf19.1926 0.88 48.79 SEF2 orf19.974 0.99 48.72 ROT2 orf19.4059 0.98 48.65 - Has domain(s) with predicted catalytic activity and role in metabolic process Putative mitochondrial fumarate reductase; regulated by Ssn6p, Gcn2p, and Gcn4p; Hog1p-downregulated; stationary phase enriched protein; Hap43prepressed gene Ortholog(s) have role in cellular response to zinc ion and cytosol, nucleus localization Ortholog of C. parapsilosis CDC317 : CPAR2_704100 Ortholog(s) have role in regulation of mitotic metaphase/anaphase transition and cytosol, nucleus localization Transcription factor required for mating and hyphal growth on solid media; in filamentation pathway with Ess1p and Czf1p; required with Efg1p for host cytokine response; regulates genes of galactose metabolism Translation elongation factor TU; macrophage/pseudohyphal-induced; genes encoding ribosomal subunits, translation factors, tRNA synthetases are downregulated upon phagocytosis by murine macrophage; levels decrease in stationary phase cells Protein with similarity to protein kinase C inhibitor-I; protein level decreases in stationary phase cultures Putative histone H3; amphotericin B repressed; regulated by Efg1p; farnesol regulated; Hap43p-induced Protein similar to S. cerevisiae Emp46p; transcription is induced in response to alpha pheromone in SpiderM medium ATP synthase alpha subunit; antigenic in human/mouse; at hyphal surface, not yeast; induced by ciclopirox, ketoconazole, flucytosine; downregulated by Efg1p, caspofungin; may be essential; sumoylation target; stationary phase-enriched Putative kynurenine 3-monooxygenase, involved in NAD biosynthesis; transposon mutation affects filamentous growth; Hap43p-repressed gene; oral infection upregulated; mutants have reduced capacity to damage oral epithelial cells Putative phosphatidylinositol kinase; non-sex gene located within the MTLa mating-type-like locus; planktonic growth-induced gene Small RAN G-protein; essential; prenylation not predicted; overproduction rescues S. cerevisiae gsp1 viability; macrophage/pseudohyphal-induced; not transcriptionally regulated by white-opaque or yeast-hyphal switching; GlcNAcinduced Ortholog(s) have DNA-directed RNA polymerase activity, RNA-directed RNA polymerase activity Ortholog(s) have Seh1-associated complex, cytosol, extrinsic to fungal-type vacuolar membrane localization Ortholog(s) have mitochondrion localization Putative histone H2A; amphotericin B repressed; flucytosine induced; RNA abundance regulated by tyrosol and cell density Has domain(s) with predicted role in transmembrane transport and integral to membrane localization Predicted ORF in Assemblies 19, 20 and 21; transcription detected in highresolution tiling array experiments Predicted ORF in Assemblies 19, 20 and 21; induced by nitric oxide Ortholog of C. parapsilosis CDC317 : CPAR2_602930 Putative zinc cluster protein, required for normal resistance to copper; expression is repressed by Sfu1p under high-iron conditions; regulated by Sef1p, Sfu1p, and Hap43p Alpha-glucosidase II, catalytic subunit, required for N-linked protein glycosylation and normal cell wall synthesis; alkaline downregulated Ortholog(s) have role in actin cortical patch localization, arginine transport, cellular protein localization, cellular response to heat, endocytosis and late endosome to vacuole transport, more Putative protein involved in the structural stability of L-A double-stranded RNAcontaining particles; downregulated upon adherence to polystyrene Putative ER-derived vesicle protein, COPII-coated vesicle complex subunit; transcription is induced upon filamentous growth orf19.4548 0.98 48.62 MAK32 orf19.586 0.97 48.61 ERV46 orf19.3342 0.99 48.57 - orf19.3001 0.94 48.57 TEM1 orf19.2124 0.85 48.54 - orf19.5866 0.98 48.53 - orf19.3402 0.99 48.53 FPG1 orf19.5968 0.92 48.46 RDI1 orf19.1180 0.99 48.36 - orf19.5806 0.97 48.32 ALD5 orf19.7544 0.98 48.31 TLO1 orf19.6931 0.99 48.23 - orf19.7483 0.93 48.18 CRM1 orf19.6883 0.90 48.16 - orf19.6132 0.98 48.15 - orf19.1166 0.99 48.14 CTA3 orf19.7136 0.99 48.14 SPT6 orf19.1409.1 0.97 48.11 - orf19.7152 0.98 48.10 - orf19.5876 0.99 48.04 - orf19.3599 0.97 47.97 TIF4631 orf19.4936 0.94 47.94 - - orf19.811 1.00 47.94 - Protein not essential for viability; Hap43p-repressed gene orf19.881 1.00 47.90 DFG16 orf19.1374 0.98 47.79 - - orf19.3437 0.98 47.61 - Has domain(s) with predicted serine-type endopeptidase activity and role in proteolysis Ortholog of C. parapsilosis CDC317 : CPAR2_109300 Protein similar to S. cerevisiae Tem1p, which is a GTPase involved in termination of the M phase of the cell cycle; induced under Cdc5p depletion Has domain(s) with predicted nucleotide binding, oxidoreductase activity, transferase activity, transferring acyl groups other than amino-acyl groups, zinc ion binding activity and role in oxidation-reduction process Protein not essential for viability Formamidopyrimidine DNA glycosylase, involved in repair of gamma-irradiated DNA; Hap43p-repressed gene Putative rho GDP dissociation inhibitor; transposon mutation affects filamentous growth; farnesol-, filament-induced; regulated by Nrg1p, Tup1p; protein levels decrease in stationary phase yeast cultures Ortholog(s) have 2-aminoadipate transaminase activity and cytoplasm, nucleus localization NAD-aldehyde dehydrogenase; decreased expression in fluconazole-resistant isolate, or in hyphae; biofilm induced; fluconazole-downregulated; protein abundance is affected by URA3 expression in the CAI-4 strain; stationary phase enriched Member of a family of telomere-proximal genes of unknown function; hyphalinduced expression Putative cleavage factor I subunit; heterozygous null mutant exhibits hypersensitivity to parnafungin and cordycepin in the C. albicans fitness test Functional homolog of S. cerevisiae Crm1p, which acts in protein nuclear export; predicted to be resistant to antifungal drug leptomycin B; partially suppresses signal transduction defects of S. cerevisiae ste20 mutant Ortholog(s) have oxysterol binding, phosphatidic acid binding, phosphatidylinositol-4,5-bisphosphate binding, phosphatidylinositol-4-phosphate binding, sterol transporter activity Ortholog of C. parapsilosis CDC317 : CPAR2_701740 Protein similar to S. cerevisiae Ede1p, which is involved in endocytosis; activates transcription in 1-hybrid assay in S. cerevisiae Protein similar to S. cerevisiae Spt6p transcription elongation factor; transposon mutation affects filamentous growth; transcription is upregulated in an RHE model of oral candidiasis and in clinical isolates from oral candidiasis Ortholog(s) have structural constituent of ribosome activity, role in cytoplasmic translation and cytosolic large ribosomal subunit, nucleolus localization Protein similar to Aspergillus CYSK O-acetylserine sulfhydrylase, suggesting that C. albicans uses an O-acetyl-serine (OAS) pathway of sulfur assimilation; upregulated in biofilm; protein level decreases in stationary phase cultures Predicted ORF in Assemblies 19, 20 and 21; greater mRNA abundance observed in a cyr1homozygous null mutant than in wild type; transcription is induced in response to alpha pheromone in SpiderM medium Putative translation initiation factor eIF4G; overexpression causes hyperfilamentation; hyphal- and macrophage-induced; genes encoding some translation factors are downregulated upon phagocytosis by murine macrophage Rim101p pathway protein that regulates filamentation in response to alkaline pH; required for host tissue invasion during infection; similar to S. cerevisiae Dfg16p that acts in Rim101p processing; late-stage biofilm-induced orf19.4463 0.99 47.60 - - orf19.7260 0.96 47.54 - Hap43p-repressed gene orf19.1266 0.96 47.51 - orf19.3730 0.99 47.49 - orf19.5034 0.98 47.36 - orf19.6234 0.99 47.32 - orf19.7140 0.99 47.28 - orf19.6674 0.99 47.24 BTS1 orf19.6100 0.98 47.21 - orf19.7115 0.98 47.21 SAC7 orf19.6621 0.96 47.19 MHP1 orf19.6658 0.94 47.17 - orf19.6183 0.99 47.13 - orf19.7085 0.86 47.10 - orf19.1121 0.99 47.09 - - orf19.6491 0.81 47.08 - - orf19.7271 0.98 47.06 orf19.3249 0.90 47.03 LAG1 orf19.6778 0.94 46.95 - orf19.5607 0.98 46.95 - Ortholog of C. parapsilosis CDC317 : CPAR2_601140 orf19.6250 0.98 46.94 - Ortholog(s) have role in mitotic cell cycle G2/M transition DNA damage checkpoint and cytoplasm localization orf19.30 0.99 46.88 - Putative calcium-transporting ATPase orf19.7161 0.98 46.87 SUI3 orf19.6193 0.97 46.81 TAF145 orf19.7670 0.99 46.77 - orf19.1991 0.85 46.66 - orf19.3885 0.96 46.66 - orf19.5834 0.92 46.63 - Predicted ORF in Assemblies 19, 20 and 21; transcription detected in highresolution tiling array experiments Protein similar to S. cerevisiae Kre27p, which has a role in resistance to killer toxin; predicted Kex2p substrate Ortholog(s) have role in cellular response to hydrogen peroxide and cytoplasm localization Putative U2 snRNP component; Hap43p-induced gene; mutation confers hypersensitivity to 5-fluorocytosine (5-FC), 5-fluorouracil (5-FU), and tubercidin (7deazaadenosine) Putative catechol o-methyltransferase; stationary phase enriched protein; transcription upregulated in clinical isolates from HIV+ patients with oral candidiasis Putative geranylgeranyl diphosphate synthase; decreased transcription is observed upon benomyl treatment Protein similar to S. cerevisiae Crd1p, a cardiolipin synthase; transcription is upregulated in clinical isolates from HIV+ patients with oral candidiasis; not essential for viability Putative GTPase activating protein (GAP) for RHO1; downregulated upon adherence to polystyrene ; macrophage/pseudohyphal-repressed; transcription is upregulated in RHE model of oral candidiasis and in clinical oral candidiasis Protein similar to S. cerevisiae Mhp1p, which is involved in microtubule stabilization; transposon mutation affects filamentous growth; possibly transcriptionally regulated upon hyphal formation; possibly an essential gene (by UAU1 method) Stationary phase enriched protein; predicted ORF from Assembly 19; removed from Assembly 20; subsequently reinstated in Assembly 21 based on comparative genome analysis Ortholog(s) have protein transporter activity, role in protein import into mitochondrial inner membrane and cytosol, mitochondrial intermembrane space protein transporter complex localization Predicted ORF in Assemblies 19, 20 and 21; induced in core stress response; induced by heavy metal (cadmium) stress via Hog1p; oxidative stress-induced via Cap1p; induced by Mnl1p under weak acid stress; macrophage-downregulated Predicted ORF in Assemblies 19, 20 and 21; transcription detected in highresolution tiling array experiments Putative ceramide synthase component; Hap43p-repressed gene; amphotericin B repressed Ortholog(s) have phospholipid-translocating ATPase activity and role in endocytosis, intracellular r protein transport, phospholipid translocation, post-Golgi vesicle-mediated transport, ribosomal small subunit assembly Putative translation initiation factor; genes encoding ribosomal subunits, translation factors, and tRNA synthetases are downregulated upon phagocytosis by murine macrophage Protein similar to S. cerevisiae Taf145p, a component of RNA polymerase II transcription factor TFIID; flucytosine repressed; likely to be essential for growth, based on an insertional mutagenesis strategy Putative Ca2+/H+ antiporter; oral infection upregulated gene; mutants have reduced capacity to damage oral epithelial cells Ortholog(s) have cytoplasm localization Has domain(s) with predicted N-acetyltransferase activity and role in metabolic process Ortholog(s) have protein transporter activity, role in mRNA export from nucleus and cytoplasm, nucleus localization orf19.7572 0.95 46.62 SPT7 Putative SAGA transcriptional regulatory complex subunit; mutation confers hypersensitivity to toxic ergosterol analog, and to amphotericin B orf19.5618 0.98 46.61 - Has domain(s) with predicted role in vesicle docking involved in exocytosis orf19.6927 0.97 46.55 PEP8 orf19.880 0.99 46.53 - orf19.5114.1 0.96 46.52 - orf19.797 0.92 46.48 BAT21 orf19.6403 0.99 46.42 - orf19.3714 0.99 46.34 - Ortholog of C. parapsilosis CDC317 : CPAR2_201980 orf19.599 0.91 46.32 - - orf19.4041 0.94 46.27 PEX4 orf19.5006.1 0.89 46.11 - orf19.6547 0.90 46.10 - orf19.819 0.98 46.01 - orf19.4045 0.99 45.98 EST1 orf19.1381 0.98 45.95 - orf19.1458 0.99 45.89 - orf19.7279.1 0.70 45.87 - Ortholog of C. parapsilosis CDC317 : CPAR2_200890, CPAR2_201160 orf19.5402 0.85 45.87 - ORF Predicted by Annotation Working Group; overlaps orf19.5401 orf19.4903 0.86 45.86 - Ortholog(s) have endoplasmic reticulum localization orf19.434 0.99 45.85 PRD1 orf19.1744 0.93 45.85 HEM4 orf19.7098 0.97 45.78 - orf19.1773 0.99 45.78 RAP1 orf19.1267 0.96 45.72 - orf19.6947 0.99 45.68 GTT11 orf19.3124 0.99 45.68 - orf19.4043 0.99 45.59 - orf19.342 0.98 45.52 BMT7 orf19.4993 0.96 45.48 SEC18 orf19.7288 0.97 45.47 - Hap43p-repressed gene orf19.5525 0.98 45.43 - Protein abundance is affected by URA3 expression in the CAI-4 strain background; regulated by Efg1p and Efh1p; repressed by Rgt1p; protein present in exponential and stationary growth phase yeast cultures Protein similar to S. cerevisiae Pep8p, which is involved in retrograde transport; transposon mutation affects filamentous growth Ortholog(s) have role in cargo loading into COPII-coated vesicle and COPII vesicle coat, cytosol, mating projection tip localization Ortholog of C. parapsilosis CDC317 : CPAR2_206910 Putative branched chain amino acid aminotransferase; regulated by Gcn4p, Gcn2p; induced in response to amino acid starvation (3-aminotriazole treatment); early-stage biofilm-induced Ortholog(s) have adenyl-nucleotide exchange factor activity, role in SRPdependent cotranslational protein targeting to membrane, translocation and endoplasmic reticulum localization Putative peroxisomal ubiquitin conjugating enzyme; transcription is upregulated in both intermediate and mature biofilms; regulated by Sef1p-, Sfu1p-, and Hap43p Ortholog(s) have SNAP receptor activity and role in Golgi to plasma membrane transport, cell cycle cytokinesis, endocytosis, exocytosis, vesicle fusion Ortholog(s) have role in lipoate biosynthetic process and mitochondrion localization Telomerase subunit; allosteric activator of catalytic activity, but not required for catalytic activity; has TPR domain Ortholog(s) have role in actin cortical patch localization, actin filament organization, endocytosis and actin cortical patch, spindle pole body localization Ortholog(s) have tRNA-specific adenosine deaminase activity, role in tRNA modification and cytoplasm, nucleus localization Putative proteinase; transcription is regulated by Nrg1p, Mig1p, and Tup1p; Hog1p-induced; stationary phase enriched protein; Hap43p-repressed gene Putative uroporphyrinogen III synthase; transcriptionally regulated by iron; expression greater in high iron or elevated CO2; alkaline downregulated; Hap43prepressed Putative protein of unknown function; Hap43p-repressed gene; repressed by nitric oxide Transcriptional regulator that binds telomeres and regulatory sequences in DNA; involved in telomere maintenance; represses hyphal growth under yeast-favoring conditions; similar to (but shorter than) S. cerevisiae Rap1p Has domain(s) with predicted heat shock protein binding, unfolded protein binding activity and role in protein folding Glutathione S-transferase, localized to ER; induced in exponentially growing cells, under oxidative stress; induced by nitric oxide Ortholog(s) have mRNA binding, metalloaminopeptidase activity, role in negative regulation of gene expression, protein initiator methionine removal involved in protein maturation and cytosolic ribosome, nucleolus localization Predicted ORF in Assemblies 19, 20 and 21; transcription is induced in response to alpha pheromone in SpiderM medium Beta-mannosyltransferase, member of a 9-gene family that includes characterized genes BMT1, BMT2, BMT3, and BMT4 with roles in beta-1,2-mannosylation of cell wall phosphopeptidomannan; downregulated in azole-resistant strain; Hap43p-induced Functional homolog of S. cerevisiae Sec18p, which acts in protein transport; conserved ATP binding site; not glycosylated orf19.6635 0.98 45.39 - Ortholog of C. parapsilosis CDC317 : CPAR2_205340 orf19.4032 0.97 45.34 RPN5 orf19.6994 0.96 45.27 BAT22 orf19.4785 0.99 45.27 PTC1 orf19.4044 0.99 45.23 MUM2 orf19.4122 0.94 45.19 - orf19.4015 0.98 45.15 CAG1 orf19.423 0.98 45.05 - orf19.795 0.95 45.03 VPS36 orf19.5076.1 0.93 45.00 - orf19.4040 0.97 44.97 ILV3 orf19.7103 0.99 44.96 - Has domain(s) with predicted nucleic acid binding activity orf19.4306 0.99 44.86 - Ortholog(s) have role in L-methionine salvage from methylthioadenosine and cytoplasm localization orf19.6748 0.98 44.79 - Ortholog(s) have role in ascospore formation, cellular response to caffeine orf19.2135 0.99 44.77 TSM1 orf19.548 0.99 44.74 CDC10 orf19.7329 0.96 44.72 - orf19.3093 0.98 44.67 MSH2 orf19.7092 0.94 44.65 - orf19.1649 0.98 44.62 RNA1 orf19.3308 0.99 44.57 STB5 orf19.7043.1 0.93 44.55 ACB1 orf19.5321 0.94 44.55 - orf19.2774 0.96 44.54 LAB5 orf19.1776 0.96 44.51 - orf19.6182 0.99 44.51 ZCF34 orf19.505 0.99 44.49 SRV2 orf19.5555 0.90 44.47 - - orf19.4523 0.99 44.42 - Ortholog(s) have 5-formyltetrahydrofolate cyclo-ligase activity, role in folic acidcontaining compound biosynthetic process and mitochondrion localization orf19.7402 0.97 44.42 DOT1 Putative COP9 signalosome component; macrophage/pseudohyphal-repressed Putative branched chain amino acid aminotransferase; regulated by Gcn4p; induced by farnesol treatment, GlcNAc, amino acid starvation (3-aminotriazole treatment); present in exponential and stationary growth phase yeast cultures Putative protein phosphatase of the Type 2C-related family (serine/threoninespecific), similar to S. cerevisiae Ptc1p; mutant shows virulence defect Protein similar to S. cerevisiae Mum2p; transcription is induced in response to alpha pheromone in SpiderM medium; transcription is regulated by Tup1p Ortholog(s) have acyl-CoA hydrolase activity, role in fatty acid beta-oxidation and mitochondrion, peroxisome localization Alpha subunit of heterotrimeric G protein; positive role in mating pheromone response; more mRNA in opaque than white cells; transcriptionally repressed by MTLa1p-MTLalpha2p complex; regulated by hemoglobin-responsive Hbr1p via MTL genes Ortholog(s) have enzyme activator activity, m7G(5')pppN diphosphatase activity, mRNA binding activity, role in deadenylation-dependent decapping of nucleartranscribed mRNA and cytoplasmic mRNA processing body localization ESCRT II protein sorting complex subunit involved in Rim8 processing and proteolytic activation of Rim101p, which regulates pH response; role in uptake of Histatin-5 into vacuole Ortholog of C. parapsilosis CDC317 : CPAR2_804320 Putative dihydroxyacid dehydratase; upregulated in biofilm; repressed by nitric oxide; macrophage-induced protein; farnesol-downregulated; protein present in exponential and stationary growth phase; Sef1p-, Sfu1p-, and Hap43p-regulated Putative transcription initiation factor TFIID subunit; transcription is upregulated in clinical isolates from HIV+ patients with oral candidiasis; transcription is regulated by Nrg1p Septin, required for wild-type cell, hyphal, or chlamydospore morphology; role in virulence and kidney tissue invasion in a mouse model of systemic infection; forms ring at sites of cell division and filaments in mature chlamydospore Ortholog(s) have ubiquitin-protein ligase activity Putative DNA mismatch repair factor; transcription is regulated by Nrg1p; flucytosine repressed; RNA abundance regulated by tyrosol and cell density Ortholog(s) have mitochondrion localization Putative GTPase-activating protein; protein level decreases in stationary phase cultures Putative transcription factor with zinc cluster DNA-binding motif; has similarity to S. cerevisiae Stb5p; Hap43p-repressed gene Protein similar to a region of acyl-coenzyme-A-binding protein; amphotericin B repressed; regulated by Nrg1p, Tup1p; Hap43p-induced Ortholog(s) have methylenetetrahydrofolate reductase (NADPH) activity and role in methionine biosynthetic process, oxidation-reduction process Ortholog(s) have role in protein lipoylation and mitochondrion localization Ortholog(s) have role in coenzyme A biosynthetic process and cytosol, nucleus localization Transcription factor with zinc cluster DNA-binding motif; null mutant displays fluconazole sensitivity; Hap43p-repressed gene; mutants display decreased colonization of mouse kidneys; required for yeast cell adherence to silicone substrate Adenylate cyclase-associated protein that regulates adenylate cyclase activity; required for wild-type germ tube formation and for virulence in mouse systemic infection; mutant defects in filamentous growth are rescued by cAMP or dbcAMP Putative modulator of white-opaque switching Nitric oxide dioxygenase, acts in nitric oxide scavenging/detoxification; role in virulence in mouse; transcription activated by NO, macrophage interaction; hyphal downregulated; mRNA binds to She3p; Hap43p-repressed orf19.3707 0.93 44.36 YHB1 orf19.3487 0.97 44.31 - Ortholog of C. parapsilosis CDC317 : CPAR2_701980 orf19.2710 0.97 44.29 - Ortholog(s) have enzyme activator activity, role in chromosome segregation and cytoplasm, nucleus localization orf19.6147 0.99 44.21 - - orf19.2092 0.99 44.18 - Putative peroxisomal cystathionine beta-lyase; Gcn4p-regulated orf19.2204 0.99 44.15 - Has domain(s) with predicted membrane localization orf19.550 0.99 44.08 PDX3 orf19.551 0.99 44.08 - orf19.3294 0.97 44.03 MBF1 orf19.6080 0.95 43.99 - Ortholog of C. parapsilosis CDC317 : CPAR2_109670 orf19.7060 0.94 43.99 - Ortholog of C. parapsilosis CDC317 : CPAR2_301080 orf19.7139 0.97 43.97 - Ortholog of C. parapsilosis CDC317 : CPAR2_703520 orf19.703 0.99 43.96 - orf19.504 0.99 43.94 - orf19.7269 0.96 43.94 - orf19.357 0.96 43.87 - orf19.593 0.99 43.84 FGR32 orf19.4005 0.99 43.80 - orf19.2125 0.79 43.73 - orf19.1592 0.99 43.69 - orf19.3453 0.96 43.67 - orf19.6437 1.00 43.65 CDC23 orf19.7097 0.99 43.58 - orf19.3181.1 0.92 43.55 - Ortholog(s) have role in protein secretion orf19.374 0.99 43.52 - Ortholog(s) have role in protein targeting to vacuole involved in ubiquitindependent protein catabolic process via the multivesicular body sorting pathway orf19.6569 0.99 43.52 - Has domain(s) with predicted role in transmembrane transport orf19.5184 0.99 43.47 - Ortholog(s) have Ran guanyl-nucleotide exchange factor activity, signal transducer activity orf19.1897 0.98 43.36 - Ortholog of C. parapsilosis CDC317 : CPAR2_105040 orf19.2994 0.96 43.32 RPL13 orf19.5534 0.99 43.30 - orf19.1584 0.98 43.25 - orf19.2151 0.99 43.21 NAG6 Pyridoxamine-phosphate oxidase; transcription regulated by yeast-hyphal switch and by Nrg1p, Mig1p, Tup1p; caspofungin repressed; present in exponential and stationary phase yeast cultures; Hap43p-repressed; planktonic growth-induced Ortholog of C. parapsilosis CDC317 : CPAR2_204170 Putative transcriptional coactivator; caspofungin repressed Ortholog(s) have 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity, role in cyclic nucleotide metabolic process and clathrin-coated vesicle localization Ortholog(s) have ATP-dependent 3'-5' DNA helicase activity, role in nucleosome mobilization and Ino80 complex, cytosol localization Ortholog(s) have aralkylamine N-acetyltransferase activity, diamine Nacetyltransferase activity, role in chromatin organization and cytoplasm localization Ortholog(s) have role in inner mitochondrial membrane organization, mitochondrion inheritance, mitochondrion morphogenesis, negative regulation of proteolysis, protein folding, replicative cell aging Protein similar to S. cerevisiae Swa2p; induced upon adherence to polystyrene; transposon mutation affects filamentous growth; Hap43p-repressed gene Putative dephospho-CoA kinase; protein likely to be essential for growth, based on an insertional mutagenesis strategy GlcNAc-induced protein Ortholog of C. glabrata CBS138 : CAGL0L12628g and C. parapsilosis CDC317 : CPAR2_212820 Has domain(s) with predicted role in signal transduction and intracellular localization Similar to anaphase-promoting complex component; possibly transcriptionally regulated by Tac1p Putative cytoplasmic RNA-binding protein; heterozygous null mutant exhibits hypersensitivity to parnafungin and cordycepin in the C. albicans fitness test Putative ribosomal subunit; antigenic during murine systemic infection; genes encoding cytoplasmic ribosomal subunits, translation factors, and tRNA synthetases are downregulated upon phagocytosis by murine macrophage Ortholog(s) have role in mitotic spindle elongation, vesicle-mediated transport and integral to Golgi membrane localization Predicted membrane transporter, member of the monocarboxylate porter (MCP) family, major facilitator superfamily (MFS); shows colony morphology-related gene regulation by Ssn6p; late-stage biofilm-induced gene Protein required for wild-type mouse virulence and wild-type cycloheximide resistance; putative GTP-binding motif; similar to S. cerevisiae Yor165Wp; in gene cluster that encodes enzymes of GlcNAc catabolism; no human or murine homolog Mo25 family domain protein of RAM cell wall integrity signaling network; role in cell separation, azole sensitivity; required for hyphal growth Predicted ORF in Assemblies 19, 20 and 21; cell-cycle regulated periodic mRNA expression Putative enoyl reductase involved in very long chain fatty acid elongation; possibly an essential gene, disruptants not obtained by UAU1 method Putative phosphatidyl synthase; stationary phase enriched protein; transcription reduced upon yeast-hyphal switch; Hap43p-repressed gene; biofilm-induced Ortholog(s) have SNAP receptor activity, role in ER to Golgi vesicle-mediated transport, intra-Golgi vesicle-mediated transport, vesicle fusion with Golgi apparatus and SNARE complex, cis-Golgi network, integral to membrane localization Putative transporter; fungal-specific; similar to Nag3p and to S. cerevisiae Ypr156Cp and Ygr138Cp; required for wild-type mouse virulence and wild-type cycloheximide resistance; gene cluster encodes enzymes of GlcNAc catabolism RecQ-related DNA helicase; Bloom's syndrome-related gene; haploinsufficient for wild-type lifespan; upregulated in farnesol treated biofilm Plasma membrane pH-sensor involved in the Rim101p pH response pathway; required for processins and activation of Rim101p and for alkaline pH-induced hyphal growth Ortholog(s) have role in mitochondrial respiratory chain complex IV assembly and mitochondrial inner membrane localization orf19.796 0.96 43.14 HYM1 orf19.2465 0.98 43.10 POL32 orf19.3293 0.96 43.07 - orf19.449 0.92 42.97 - orf19.6039 0.99 42.97 - orf19.2160 0.96 42.93 NAG4 orf19.5335 0.94 42.83 SGS1 orf19.3176 0.96 42.81 RIM21 orf19.7665 0.93 42.79 - orf19.3027 0.99 42.76 - Ortholog(s) have DNA-dependent ATPase activity orf19.450 0.95 42.73 - - orf19.6758 0.99 42.67 - Ortholog(s) have cytosol, nucleus localization orf19.6724 0.98 42.64 FUM12 orf19.3233 1.00 42.60 HSE1 orf19.6537 0.99 42.59 - orf19.339 0.91 42.55 NDE1 orf19.6406 0.98 42.46 - orf19.5006 0.90 42.43 GCV3 orf19.2149 0.98 42.41 - orf19.6949 0.97 42.33 - - orf19.5078 0.88 42.24 - Hap43p-repressed gene orf19.6393 0.99 42.22 - orf19.5052 0.99 42.17 - orf19.7389 0.97 42.10 REV3 orf19.2766 0.97 42.09 - - orf19.3916 0.99 42.05 - Ortholog of C. parapsilosis CDC317 : CPAR2_100980 orf19.5602 0.93 42.00 BMT6 orf19.3658 0.96 41.97 - Putative fumarate hydratase, enzyme of citric acid cycle; fluconazoledownregulated; downregulated by Efg1p; transcriptionally regulated by iron; expression greater in high iron; protein present in exponential and stationary growth phase ESCRT-0 complex subunit; SH3-domain-containing protein Ortholog(s) have role in chromosome segregation and nuclear MIS12/MIND type complex, spindle pole localization Putative NADH dehydrogenase that could act alternatively to complex I in respiration; caspofungin repressed; fungal-specific (no human or murine homolog); transcription is upregulated in both intermediate and mature biofilms Ortholog(s) have role in regulation of sporulation resulting in formation of a cellular spore and cytosol, mitochondrion, nucleus localization Glycine decarboxylase, subunit H; protein level decrease in stationary phase cultures Putative sulfate permease; S. cerevisiae ortholog YPR003C localizes to the endoplasmic reticulum; regulated by Sef1p-, Sfu1p-, and Hap43p Putative Arf3p GTPase activating protein; Hap43p-repressed gene; possibly an essential gene, disruptants not obtained by UAU1 method Ortholog of C. glabrata CBS138 : CAGL0C02013g and C. parapsilosis CDC317 : CPAR2_208340 Ortholog(s) have DNA-directed DNA polymerase activity, role in error-free translesion synthesis, error-prone translesion synthesis and cytosol, mitochondrion, nuclear chromatin, nucleus, zeta DNA polymerase complex localization Putative beta-mannosyltransferase, member of a 9-gene family that includes characterized genes BMT1, BMT2, BMT3, and BMT4 with roles in beta-1,2mannosylation of cell wall phosphopeptidomannan; biofilm- and planktonic growth-induced gene Ortholog(s) have role in protein folding in endoplasmic reticulum and Golgi apparatus, endoplasmic reticulum, mitochondrion localization Predicted membrane transporter, member of the aromatic acid:proton symporter (AAHS) family, major facilitator superfamily (MFS) Phosphorylated protein similar to S. cerevisiae Apm1, which is involved in vesicle transport; transposon mutation affects filamentous growth Ortholog(s) have protein binding, bridging activity and role in ER-associated protein catabolic process, protein secretion Predicted ortholog of S. cerevisiae Tfp3p, which is the c' subunit of the V0 subcomplex of the vacuolar ATPase; required for hemoglobin-iron utilization orf19.6592 0.98 41.96 - orf19.4036 0.99 41.93 APM1 orf19.3135 0.98 41.92 - orf19.6538 0.99 41.90 VMA11 orf19.7305 0.96 41.89 - orf19.6176 0.99 41.87 SEC61 orf19.4106 0.99 41.85 - orf19.216 0.98 41.85 - orf19.4675 0.98 41.80 ASK1 orf19.396 0.96 41.79 EAF6 orf19.6612 0.91 41.78 - Putative mitochondrial protein; Hap43p-induced gene orf19.3659 0.96 41.70 - Ortholog(s) have CTD phosphatase activity, role in dephosphorylation of RNA polymerase II C-terminal domain, transcription from RNA polymerase II promoter and cytoplasm, nucleus localization orf19.3517 1.00 41.53 - Ortholog(s) have endoplasmic reticulum, fungal-type vacuole lumen localization orf19.2094 0.98 41.53 PDR6 orf19.4600.1 0.99 41.52 - orf19.2994.1 0.96 41.49 RPS16A orf19.5564 0.98 41.49 - orf19.6626 0.99 41.38 - orf19.7030 0.94 41.36 SSR1 orf19.175 0.99 41.31 - - orf19.384 0.94 41.30 - Ortholog(s) have Golgi apparatus, cytosol, nucleus localization orf19.2307 0.97 41.27 - Ortholog(s) have role in mRNA cleavage, mRNA polyadenylation, mitotic sister chromatid segregation, regulation of G1/S transition of mitotic cell cycle and cytosol, mRNA cleavage and polyadenylation specificity factor complex localization orf19.2037 0.94 41.16 - Ortholog of C. parapsilosis CDC317 : CPAR2_212950 orf19.4241 0.99 41.14 - orf19.946 0.94 41.14 MET14 orf19.6194 0.97 41.13 - ER protein-translocation complex subunit; essential; similar to S. cerevisiae Sec61p; 10 transmembrane regions predicted; chimeric mutant partially functionally complements S. cerevisiae sec61 defects Ortholog of S. cerevisiae : YJR141W, C. glabrata CBS138 : CAGL0M00264g and C. parapsilosis CDC317 : CPAR2_104880 Predicted ORF in Assemblies 19, 20 and 21; ketoconazole-induced; upregulation correlates with clinical development of fluconazole resistance Essential subunit of the Dam1 (DASH) complex, which acts in chromosome segregation by coupling kinetochores to spindle microtubules Subunit of the NuA4 histone acetyltransferase complex; Hap43p-repressed gene Putative pleiotropic drug resistance regulatory protein 6; transcription is regulated by Nrg1p; transcription is induced in response to alpha pheromone in SpiderM medium Ortholog of C. parapsilosis CDC317 : CPAR2_400490 Putative 40S ribosomal subunit; macrophage/pseudohyphal-induced after 16 h Ortholog(s) have nucleolus localization Predicted ORF in Assemblies 19, 20 and 21; possibly transcriptionally regulated upon hyphal formation Beta-glucan associated ser/thr rich cell-wall protein with a role in cell wall structure; GPI anchor; similar mRNA abundance in yeast-form and germ tubes; detected at germ tube plasma membrane; repressed in cells treated with Congo Red Predicted ORF in Assemblies 19, 20 and 21; transcription detected in highresolution tiling array experiments Putative adenylylsulfate kinase; predicted role in sulfur metabolism; induced upon biofilm formation; possibly adherence-induced; protein present in exponential and stationary growth phase yeast cultures Protein not essential for viability Ketol-acid reductoisomerase; antigenic in human/murine infection; regulated by Gcn4p; GlcNAc, amino acid starvation (3-AT)-induced; biofilm induced; macrophage-downregulated protein; protein present in exponential and stationary phase Ortholog(s) have SUMO activating enzyme activity, role in protein sumoylation, regulation of mitotic cell cycle, response to DNA damage stimulus and SUMO activating enzyme complex, cytosol localization orf19.88 0.93 41.08 ILV5 orf19.2835 0.98 41.02 - orf19.6326 0.95 40.99 - Ortholog of C. parapsilosis CDC317 : CPAR2_603380 orf19.1340 0.98 40.92 - Putative aldose reductase; protein level decreases in stationary phase cultures orf19.4288 0.99 40.90 CTA7 orf19.6858 0.98 40.88 EDC3 orf19.7081 0.99 40.84 SPL1 orf19.5420 0.99 40.82 - orf19.6742 0.98 40.80 - orf19.1582 0.82 40.78 - orf19.6554 0.57 40.77 - orf19.1377 0.94 40.76 IPK2 orf19.961.2 1.00 40.75 - orf19.3713 0.95 40.74 - orf19.1992 0.95 40.72 SIR2 orf19.3988 0.71 40.71 - orf19.3182 0.95 40.69 GIS2 orf19.2093 0.96 40.69 RFA1 orf19.2765 0.91 40.68 PGA62 orf19.5757 0.97 40.66 - orf19.7236 0.99 40.63 TIF35 orf19.7666 0.93 40.56 - orf19.6533 0.98 40.55 MSK1 orf19.763 0.98 40.48 - orf19.4228 0.99 40.47 - orf19.4448 0.96 40.47 SOG2 orf19.2630 0.97 40.40 RAD59 orf19.1560 0.97 40.37 POB3 orf19.7123 0.98 40.35 - orf19.5619 0.98 40.35 - orf19.5484 0.99 40.22 SER1 orf19.7484 0.97 40.21 ADE1 Predicted zinc-finger protein; activates transcription in 1-hybrid assay in S. cerevisiae; has similarity to S. cerevisiae Stb4p Ortholog(s) have role in cytoplasmic mRNA processing body assembly, deadenylation-independent decapping of nuclear-transcribed mRNA and cytoplasmic mRNA processing body localization Protein similar to S. cerevisiae Spl1p, which is involved in tRNA splicing; member of pyridoxal-phosphate-dependent aminotransferase protein family; predicted to be essential Ortholog(s) have role in fatty acid metabolic process Ortholog(s) have CTD phosphatase activity and role in cellular protein complex assembly, dephosphorylation of RNA polymerase II C-terminal domain, negative regulation of G0 to G1 transition, regulation of cell cycle cytokinesis Predicted membrane transporter, member of the drug:proton antiporter (12 spanner) (DHA1) family, major facilitator superfamily (MFS); mRNA binds to She3p Hap43p-repressed gene; expression upregulated by ketoconazole, by hypoxia and during growth in the mouse cecum Putative inositol polyphosphate multikinase; moderately induced at 42 deg Ortholog(s) have mitochondrion localization Late-stage biofilm-induced gene; transcriptionally activated by Mnl1p under weak acid stress; transcription detected in high-resolution tiling array experiments Required for wild-type lifespan, asymmetric inheritance of oxidatively damaged proteins, rDNA silencing; regulates phenotypic switching in strain 3153A, not WO1; partially functionally complements S. cerevisiae sir2 mutant mating defect Putative adhesin-like protein; transcriptionally activated by Mnl1p under weak acid stress Putative transcription factor; expression is increased in high iron and reduced upon yeast-hyphal switch; null mutant exhibits sensitivity to sorbitol, 5fluorocytosine, and cold temperatures Putative DNA replication factor A; RNA abundance regulated by cell cycle, tyrosol and cell density Adhesin-like cell wall protein; putative GPI-anchor; fluconazole-induced; regulated by iron; expression greater in high iron; induced during cell wall regeneration; Cyr1p or Ras1p downregulated; positively regulated by Tbf1p Ortholog(s) have cytoplasm, nucleus localization Putative translation initiation factor; genes encoding ribosomal subunits, translation factors, and tRNA synthetases are downregulated upon phagocytosis by murine macrophage Predicted membrane transporter, member of the anion:cation symporter (ACS) family, major facilitator superfamily (MFS) Putative mitochondrial lysine-tRNA synthetase; flucytosine repressed Ortholog(s) have RNA cap binding activity, role in nuclear mRNA splicing, via spliceosome and commitment complex, cytosol, nuclear cap binding complex, spindle localization Ortholog(s) have role in posttranslational protein targeting to membrane, protein insertion into ER membrane and TRC complex, nucleus localization Leucine-rich-repeat domain protein of RAM cell wall integrity signaling network; role in cell separation, azole sensitivity; required for hyphal growth; lacks orthologs in higher eukaryotes Protein involved in homologous recombination and DNA breaks repair; similar to S. cerevisiae Rad59p; N-terminal similarity with Rad52p Protein similar to S. cerevisiae Pob3p, which is involved in chromatin assembly and disassembly; transposon mutation affects filamentous growth Ortholog of C. parapsilosis CDC317 : CPAR2_300050 Predicted ORF in Assemblies 19, 20 and 21; transcription is induced in response to alpha pheromone in SpiderM medium Putative 3-phosphoserine aminotransferase with a predicted role in serine and glycine biosynthesis; protein present in exponential and stationary yeast growth phases Phosphoribosylaminoimadazole succinocarboxamide synthetase, enzyme of adenine biosynthesis; not induced in GCN response, unlike the S. cerevisiae ortholog; fungal-specific (no human or murine homolog); levels decrease in stationary phase orf19.6252 0.99 40.19 - orf19.7164 0.99 40.14 orf19.4683 0.97 40.13 MLP1 orf19.1190 0.95 40.03 - orf19.6091 0.96 40.02 RIM8 orf19.1453 0.99 39.98 SPT5 orf19.5821 0.96 39.95 - orf19.3957 0.99 39.95 - orf19.6822 0.95 39.88 - orf19.3656 0.76 39.84 COX15 orf19.4597 0.99 39.78 - orf19.4942 0.99 39.69 - orf19.6814 0.94 39.68 TDH3 orf19.2272 0.99 39.64 AFT2 orf19.4833 0.61 39.60 MLS1 orf19.4230 1.00 39.56 - orf19.2988 0.97 39.54 - orf19.6975 0.93 39.40 YST1 orf19.2146 0.99 39.38 - orf19.2612 0.96 39.34 - orf19.7151 0.98 39.29 - orf19.1342 0.84 39.21 SHM1 orf19.2973 0.74 38.75 - orf19.744 0.76 38.42 GDB1 orf19.3002 0.81 38.24 RPS1 orf19.6600 0.81 38.20 - orf19.7043 0.85 37.63 - orf19.7451 0.82 37.12 FUN31 orf19.5334 0.82 36.84 - Ortholog(s) have RNA polymerase II transcription factor binding transcription factor activity, core RNA polymerase II binding transcription factor activity Ortholog(s) have role in vacuolar protein processing and Golgi apparatus, endoplasmic reticulum, fungal-type vacuole membrane localization Ortholog(s) have ribonucleoprotein complex binding activity Has domain(s) with predicted hydrogen ion transmembrane transporter activity, role in ATP hydrolysis coupled proton transport and proton-transporting two-sector ATPase complex, proton-transporting domain localization beta-arrestin-like protein involved in pH response; required for pathogenesis, activation of Rim101p and alkaline pH-induced hyphal growth; colony morphology-related gene regulation by Ssn6p; negative feedback regulation target Protein similar to S. cerevisiae Spt5p transcription elongation factor; transposon mutation affects filamentous growth Ortholog of S. cerevisiae : YML002W, C. glabrata CBS138 : CAGL0L07634g and C. parapsilosis CDC317 : CPAR2_212430 Ortholog(s) have GTP cyclohydrolase I activity, role in folic acid-containing compound biosynthetic process and cytosol, nucleus localization Ortholog(s) have NEDD8 ligase activity, ubiquitin binding activity and role in positive regulation of ubiquitin-protein ligase activity, protein neddylation Cytochrome oxidase assembly protein; transcription is regulated by Nrg1p and Tup1p; alkaline downregulated; Hap43p-repressed gene; early-stage biofilminduced gene Putative F-actin-capping protein subunit beta; possibly an essential gene, disruptants not obtained by UAU1 method NAD-linked glyceraldehyde-3-phosphate dehydrogenase; glycolysis enzyme; binds fibronectin and laminin; at yeast and hyphal surface; antigenic in infection; farnesol-downregulated; stationary phase-enriched; biofilm-induced; GlcNAcinduced Putative transcription factor with a role in colony morphology and virulence; functionally complements S. cerevisiae aft1 mutant growth defect Malate synthase; glyoxylate cycle enzyme; no mammalian homolog; transcription induced upon phagocytosis by macrophage; regulated upon white-opaque switching; biofilm-repressed; strong oxidative stress induced; stationary phase enriched 20S proteasome subunit (beta7); protein present in exponential and stationary growth phase yeast cultures Biofilm-induced gene Ribosome-associated protein; antigenic in mice; complements S. cerevisiae yst1 yst2 mutant; similar to laminin-binding proteins, does not bind laminin; predicted S/T phosphorylation, N-glycosylation, myristoylation, Hap43p-, Gcn4p-regulated Ortholog(s) have histone acetyltransferase activity, histone binding activity, role in chromatin assembly or disassembly, chromatin silencing at telomere, histone acetylation and cytoplasm, histone acetyltransferase complex localization Putative zinc finger DNA-binding transcription factor; expression downregulated in an ssr1null mutant; late-stage biofilm-induced gene Mitochondrial serine hydroxymethyltransferase; complements the glycine auxotrophy of an S. cerevisiae shm1 null shm2 null gly1-1 triple mutant; protein present in exponential and stationary growth phase yeast cultures Ortholog of C. parapsilosis CDC317 : CPAR2_106110 Putative glycogen debranching enzyme; expression is regulated upon whiteopaque switching; regulated by Nrg1p, Tup1p Putative ribosomal protein 10 of the 40S subunit, elicits a host antibody response during infection; transcription is induced during active growth Ortholog(s) have role in cardiolipin metabolic process and cytosol, extrinsic to mitochondrial inner membrane, mitochondrial intermembrane space, nucleus localization Ortholog(s) have endoplasmic reticulum localization Putative PAS kinase involved in cell wall damage response; similar to S. cerevisiae Psk1p, which is a putative serine/threonine protein kinase; transcriptionally activated by Mnl1p under weak acid stress Biofilm- and planktonic growth-induced gene; transcription regulated upon yeasthyphal switch orf19.3368 0.68 36.39 - - orf19.1974 0.77 36.14 TFS1 orf19.4623.3 0.46 36.07 NHP6A orf19.6229 0.53 35.60 CAT1 orf19.4490 0.85 35.28 RPL17B orf19.489 0.58 34.88 DAP1 orf19.4318 0.83 34.37 MIG1 orf19.3223 0.64 34.18 ATP3 orf19.5711 0.86 31.99 - orf19.6794 0.85 30.75 FESUR1 orf19.6601 0.81 30.58 - orf19.1354 0.83 30.16 UCF1 Hap43p-repressed gene; mRNA binds She3p; transcription induced in high iron, decreased upon yeast-hyphal switch; downregulation correlates with clinical development of fluconazole resistance; Ras1p-regulated; biofilm-induced gene orf19.341 0.74 30.15 - Putative spermidine export pump; fungal-specific (no human or murine homolog) orf19.203 0.84 29.67 STB3 orf19.2508 0.84 29.61 PRM9 orf19.2509 0.84 29.61 - orf19.3221 0.36 29.52 CPA2 orf19.6478 0.63 28.30 YCF1 orf19.726 0.73 27.69 PPZ1 orf19.7020 0.64 27.61 - orf19.3795 0.86 27.10 AGP3 orf19.403 0.82 27.00 PCL2 orf19.4342 0.81 26.66 - orf19.1779 0.74 26.31 MP65 orf19.7001 0.85 26.17 YCK2 orf19.6763 0.83 25.87 SLK19 Putative carboxypeptidase y inhibitor; transcription is regulated upon yeast-hyphal switch; shows colony morphology-related gene regulation by Ssn6p Putative non-histone chromatin component; RNA abundance regulated by tyrosol and cell density; Hap43p-induced; amphotericin B, caspofungin repressed; intron in 5'-UTR Catalase; resistance to oxidative stress, neutrophils, peroxide; role in virulence; regulated by iron, ciclopirox, fluconazole, carbon source, pH, Rim101p, Ssn6p, Hog1p, Hap43p, Sfu1p, Sef1p, farnesol, core stress response Ribosomal protein L17; mutation confers hypersensitivity to 5-FU, tubercidin; genes encoding cytoplasmic ribosomal subunits, translation factors, tRNA synthetases are downregulated on phagocytosis by macrophage; Hap43p-induced gene Similar to mammalian membrane-associated progesterone receptors involved in DNA damage response; induced in core stress response; Hog1p regulated; cladeassociated expression; Hap43p-repressed; planktonic growth-induced gene Transcriptional repressor; regulates genes for carbon source utilization; Tup1pdependent, -independent functions; upregulated in biofilm and planktonic cultures; hyphal downregulated; Hap43p and caspofungin repressed F1-ATP synthase complex subunit; caspofungin repressed; flucytosine and macrophage/pseudohyphal-induced; present in exponential and stationary growth phases; Hap43p-induced gene Putative phosphatidylinositol transfer protein; possibly an essential gene, disruptants not obtained by UAU1 method Putative ubiquinone reductase; transcriptionally induced by interaction with macrophage; alkaline downregulated; repressed by nitric oxide; clade-associated gene expression; Hap43p-repressed gene Biofilm-induced gene Putative SIN3-binding protein 3 homolog; caspofungin induced; macrophage/pseudohyphal-repressed Protein not essential for viability; similar to S. cerevisiae Prm9p, which may bind to COPII coated vesicles Putative arginine-specific carbamoylphosphate synthetase; protein enriched in stationary phase yeast cultures; transcription is upregulated in both intermediate and mature biofilms Putative glutathione S-conjugate transporter; MRP/CFTR-subfamily, ABC transporter family; human neutrophil-induced; oxidative stress-induced via Cap1p; possible association with multidrug resistance; possibly an essential gene (UAU1 method) Protein phosphatase Z; type 1 family serine/threonine specific protein phosphatase involved in cation homeostasis and cell wall integrity Protein similar to S. cerevisiae Kex1p, which is a pheromone-processing peptidase; possible Kex2p substrate Putative serine transporter with possible role in assimilation of sulfur; induced upon early biofilm formation; fungal-specific (no human or murine homolog) Cyclin homolog; reduced expression observed upon depletion of Cln3p; farnesol regulated; periodic mRNA expression, peak at cell-cycle G1/S phase; Hap43pinduced gene Biofilm-induced gene Cell surface mannoprotein; cell-wall glucan metabolism, biofilm, adhesion; adhesin motif; O-glycosylation; induced by heat, germ tube formation, wall regeneration; mycelial antigen; diagnostic marker; fluconazole-repressed Plasma membrane protein similar to S. cerevisiae casein kinase I, Yck2p; null mutant has defect in damaging oral epithelial cells and in hyphal branching; transcription is activated in weak acid stress or on contact with host cells Alkaline-induced protein of plasma membrane; affects cell aggregation, cell wall; similar to S. cerevisiae Slk19p (a kinetochore protein with roles in mitosis, meiosis); required for wild-type virulence in mouse; macrophage-downregulated Plasma membrane H(+)-ATPase; highly expressed, comprising 20-40% of total plasma membrane protein; abundance increases at stationary phase transition; fluconazole induced; caspofungin repressed; upregulated in RHE model Similar to chaperones of Hsp70p family; role in translocation of proteins into the ER; transcriptionally regulated by iron; expression greater in high iron; protein present in exponential and stationary growth phase yeast cultures Protein not essential for viability; similar to S. cerevisiae Rod1p, which is a membrane protein with a role in drug tolerance; repressed by Rgt1p orf19.5383 0.52 25.67 PMA1 orf19.2013 0.72 25.48 KAR2 orf19.1509 0.80 25.38 ROD1 orf19.4539 0.84 24.77 - orf19.3530 0.73 24.44 CKA2 orf19.7052 0.85 24.32 - orf19.4736 0.76 24.29 - orf19.2672 0.77 24.18 NCP1 orf19.6925 0.81 24.18 HTB1 orf19.4128 0.82 24.14 - orf19.2871 0.85 24.06 SDH12 orf19.2457 0.86 23.32 - orf19.6548 0.86 22.66 ISU1 orf19.4886 0.80 22.59 - orf19.2613 0.78 22.30 ECM4 orf19.399 0.74 22.27 - orf19.336 0.75 21.86 YAH1 orf19.204 0.86 21.53 - orf19.3967 0.78 21.32 PFK1 orf19.6536 0.60 21.24 IQG1 orf19.6164 0.83 21.18 - orf19.1704 0.78 20.50 FOX3 orf19.7499 0.77 20.27 - orf19.6924 0.77 19.93 HTA1 orf19.87 0.76 19.74 GPX1 orf19.251 0.84 19.45 - ThiJ/PfpI protein; binds human immunoglobulin E; 2 N-glycosylation motifs; alkaline, fluconazole, Hog1p-downregulated; induced in core stress response or by oxidative stress (via Cap1p); induced by hypoxia, Hap43p; stationary-phase enriched orf19.3531 0.79 19.21 - Ortholog of C. parapsilosis CDC317 : CPAR2_210010 orf19.2461 0.61 18.84 PRN4 Hap43p-repressed gene Catalytic alpha-subunit of protein kinase CK2; interaction with calcineurin pathway affects fluconazole sensitivity; synthetically lethal with CKA1; attenuated virulence in a mouse oropharyngeal candidiasis but not in a systemic mouse model Putative polyphosphatidylinositol phosphatase; possibly an essential gene, disruptants not obtained by UAU1 method Ortholog(s) have alkaline phosphatase activity, nucleotide phosphatase activity, role in nicotinamide nucleotide metabolic process, protein dephosphorylation and fungal-type vacuole membrane localization NADPH-cytochrome P450 reductase, acts with Erg11p in sterol 14 alphademethylation in ergosterol biosynthesis; subject to hypoxic regulation; ketoconazole-induced; caspofungin repressed Putative histone H2B; induced upon adherence to polystyrene; amphotericin B or caspofungin repressed; flucytosine or fluconazole induced; regulated by Efg1p; slow growth, increased white-to opaque switching in ectopic expression strains Ortholog of C. parapsilosis CDC317 : CPAR2_209600 Succinate dehydrogenase; soluble protein in hyphae; macrophage-downregulated protein level; downregulated by Efg1p; repressed by nitric oxide; protein present in exponential and stationary growth phase yeast cultures; Hap43p-repressed gene Protein with similarity to NifU; possible role in iron-sulfur cluster biogenesis; transcriptionally regulated by iron; expression greater in low iron; transcription is upregulated in both intermediate and mature biofilms Putative adhesin-like protein; Hap43p-repressed gene Cytoplasmic glutathione S-transferase; transcription regulated by Nrg1p, Tup1p; induced in core stress response, in cyr1 or ras1 null mutant (yeast or hyphal cells); transposon mutation affects filamentous growth; stationary phase enriched Putative serine/threonine protein kinase; possibly an essential gene, disruptants not obtained by UAU1 method Similar to oxidoreductases; transcriptionally regulated by iron; expression greater in high iron; Hap43p-repressed gene Phosphofructokinase alpha subinit, Pfk1p-Pfk2p heteromultimer; activated by fructose 2,6-bisphosphate, AMP, inhibited by ATP; activity reduced on hyphal induction; phagocytosis-downregulated; fluconazole-induced; biofilm-induced Component of actomyosin ring at bud neck; cell-cycle regulated serine phosphorylation at CDK sites regulates association with formins Bni1p and Bnr1p, Iqg1p degradation, and ring disassembly; mutation causes severe cytokinetic defects Putative peroxisomal 3-oxoacyl CoA thiolase; expression is regulated upon whiteopaque switching Putative nicotinic acid mononucleotide adenylyltransferase, involved in NAD salvage pathway Histone H2A; reduced mRNA abundance detected in homozygous fkh2 null mutant; amphotericin B repressed; farnesol regulated; RNA abundance regulated by tyrosol and cell density; Hap43p-induced gene Putative thiol peroxidase Protein with similarity to pirins; increased transcription is observed upon benomyl treatment orf19.846 0.86 18.68 - Predicted ORF in Assemblies 19, 20 and 21; transcriptionally activated by Mnl1p under weak acid stress orf19.954 0.82 18.44 - Hap43p-repressed gene orf19.3220 0.84 18.35 - orf19.3665 0.83 18.26 - orf19.6824 0.85 18.25 TRY6 orf19.6195 0.72 18.23 - Ortholog(s) have role in endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA and LSU-rRNA), more Hap43p-induced gene; ortholog of S. cerevisiae SET6, a SET domain protein of unknown function Transcriptional regulator of yeast form adherence; required for yeast cell adherence to silicone substrate; possibly C. albicans-specific; induced in biofilm; transcription repressed in response to alpha pheromone in SpiderM medium Ortholog(s) have nucleus localization orf19.6584 0.82 17.96 PRT1 Putative translation initiation factor eIF3; mutation confers hypersensitivity to roridin A, verrucarin A; genes encoding ribosomal subunits, translation factors, tRNA synthetases are downregulated upon phagocytosis by murine macrophages orf19.3321 0.52 17.67 MRS7 Member of the LETM1-like protein family, mitochondrial membrane protein orf19.6029 0.81 17.21 ROT1 orf19.6810 0.79 16.94 - orf19.3003 0.85 16.91 - orf19.3264.1 0.68 16.57 - orf19.2883 0.85 16.55 CSO99 orf19.1993 0.84 16.23 - orf19.6385 0.77 16.06 ACO1 orf19.4997 0.83 15.74 KIS2 orf19.5622 0.79 15.73 GLC3 orf19.412 0.75 15.47 SSH1 Protein with a role in protein translocation across membranes orf19.2531 0.85 15.04 CSP37 Hyphal cell wall protein; role in progression of mouse systemic infection; predicted P-loop, divalent cation binding, N-glycosylation sites; expressed in yeast and hyphae; hyphal downregulated; stationary-phase enriched; GlcNAc-induced orf19.3929 0.84 14.56 - orf19.2843 0.83 14.47 RHO1 orf19.273 0.86 14.05 - orf19.3774.1 0.85 13.89 - orf19.3869 0.86 13.67 - orf19.4647 0.86 13.52 HAP3 orf19.1783 0.73 12.55 YOR1 Similar to S. cerevisiae Rot1p, which is involved in cell wall 1,6-beta-glucan biosynthesis; has predicted transmembrane region; possibly an essential gene, disruptants not obtained by UAU1 method Ortholog(s) have protein serine/threonine phosphatase activity and role in establishment or maintenance of cytoskeleton polarity, mitotic cell cycle spindle assembly checkpoint, regulation of translation Putative lipid-binding protein with a predicted role in calcium-dependent phospholipid-binding Hap43p-repressed gene, protein not conserved in S. cerevisiae Ortholog(s) have structural molecule activity, role in proteasome assembly, ubiquitin-dependent protein catabolic process and proteasome regulatory particle, lid subcomplex, proteasome storage granule localization Aconitase; expression greater in high iron; 2 upstream CCAAT motifs; amino acid starvation (3-AT), amphotericin B, phagocytosis, farnesol induced; fluconazoledownregulated; Gcn4p-regulated; Hap43p-repressed; antigenic in infection Scaffold protein of Snf1p complex; similar to S. cerevisiae Gal83p and Sip2p; interacts with Snf4p; interaction with Snf1p complex is regulated by carbon source, decreased on ethanol; N-terminal myristoylation; Hog1p-downregulated Putative 1,4-glucan branching enzyme; fluconazole-induced; shows colony morphology-related gene regulation by Ssn6p; stationary phase enriched protein; planktonic growth-induced gene Ortholog(s) have mitochondrion localization Small GTPase of Rho family; regulates beta-1,3-glucan synthesis activity and binds Gsc1p; essential; expected to be geranylgeranylated by geranylgeranyltransferase type I; plasma membrane-localized Ortholog(s) have unfolded protein binding activity, role in sterol biosynthetic process and endoplasmic reticulum localization Ortholog(s) have protein tag activity and role in cell morphogenesis involved in conjugation with cellular fusion, cellular protein localization, nuclear mRNA cis splicing, via spliceosome, protein modification process Predicted ORF in Assemblies 19, 20 and 21; regulated by Tsa1p, Tsa1Bp in minimal media at 37 deg; shows colony morphology-related gene regulation by Ssn6p Similar to CCAAT-binding transcription factor that regulates respiration; Cap2dependent upregulation in low iron; transcription is opaque specific, alkaline upregulated, ciclopirox olamine induced; regulated by Sef1p, Sfu1p, and Hap43p Protein similar to S. cerevisiae Yor1p, which is a plasma membrane transporter of the ATP-binding cassette (ABC) family involved in resistance to aureobasidin A; transcription is specific to white cell type orf19.413 0.85 12.51 - Transcription is positively regulated by Sfu1p orf19.4596 0.72 12.43 - Ortholog of C. parapsilosis CDC317 : CPAR2_400530, CPAR2_400450 orf19.192 0.82 12.29 - Ortholog of C. parapsilosis CDC317 : CPAR2_209670 orf19.5241 0.86 12.12 SNT1 orf19.1009 0.78 11.84 - orf19.3422 0.84 11.79 FMP27 orf19.4905 0.80 11.58 - orf19.6798 0.63 11.48 SSN6 orf19.1580 0.82 11.17 - orf19.2747 0.85 10.81 RGT1 orf19.2437 0.84 10.78 ARC35 orf19.3928 0.76 10.69 - Putative transcription factor with zinc finger DNA-binding motif Protein with similarity to S. cerevisiae Ykr070wp; transposon mutation affects filamentous growth; Hog1p-downregulated; shows colony morphology-related gene regulation by Ssn6p; induced during cell wall regeneration; possibly essential gene Protein similar to S. cerevisiae Snt1p, which is an NAD-independent histone deacetylase; not essential for viability; transposon mutation affects filamentous growth Putative mitochondrial protein; mRNA binds She3p; fungal-specific (no human or murine homolog) Putative MFS transporter; Hap43p-induced gene; also regulated by regulated by Sef1p and Sfu1p; repressed in a ssr1 null mutant Functional homolog of S. cerevisiae Cyc8p/Ssn6p; Ssn6p and Tup1p regulate distinct sets of genes; hyphal growth regulator; overexpression or mutation causes avirulence in mouse IV infection; TPR motifs; repressed during hyphal growth Transcriptional repressor involved in the regulation of glucose transporter genes; has zinc-cluster; similar to S. cerevisiae Rgt1p; mutants display decreased colonization of mouse kidneys Putative ARP2/3 complex subunit; shows colony morphology-related gene regulation by Ssn6p; mutation confers hypersensitivity to cytochalasin D orf19.4246 0.83 10.54 - orf19.695 0.77 10.45 RGS2 orf19.5369 0.86 10.18 - orf19.2435 0.86 9.75 MSI3 orf19.3629 0.83 9.45 DSE1 orf19.3426 0.86 9.44 ANB1 orf19.6638 0.80 8.84 PTC4 orf19.3237 0.79 8.60 - Ortholog(s) have cytosol, mitochondrion, nucleus localization orf19.6275 0.69 8.37 - Ortholog(s) have cytosol localization orf19.6311 0.63 8.11 - Hap43p-induced gene orf19.5073 0.82 7.94 DPM1 orf19.7016 0.84 7.89 PHM5 orf19.3209 0.86 7.72 FGR42 orf19.92 0.86 7.54 - Hap43p-repressed gene; increased expression in response to prostaglandins orf19.7024 0.79 7.53 - - orf19.4921.1 0.83 7.04 - Ortholog of C. parapsilosis CDC317 : CPAR2_801610 orf19.1075.1 0.82 7.02 - Pseudogene; added to Assembly 21 based on comparative genome analysis orf19.5801 0.49 6.11 RNR21 Ribonucleoside-diphosphate reductase; regulated by tyrosol and cell density; transcription upregulated in response to ciclopirox olamine; fluconazole or Protein of RGS superfamily; not essential for viability Ortholog(s) have uroporphyrinogen decarboxylase activity, role in heme biosynthetic process and cytosol, nucleus localization Antigenic HSP70 family protein; functional homolog of S. cerevisiae Msi3p; interacts by 2-hybrid with Cgr1p; transcriptionally regulated by iron; expression greater in high iron; farnesol downregulated in biofilm; sumoylation target Essential cell wall protein involved in cell wall integrity and rigidity; periodic mRNA expression peaks at M/G1 phase; Ace2p-induced; required for virulence in a mouse model of infection Translation initiation factor eIF-5A; decreased expression in hyphae vs yeast cells; downregulated upon phagocytosis by murine macrophage; Hap43pinduced; GlcNAc-induced protein Type PP2C serine/threonine phosphatase; localized to mitochondria; mutation causes sensitivity to sodium, potassium and azole drugs; decreased expression in hyphae compared to yeast-form cells Putative dolichol phosphate mannose synthase; filament induced; Tup1pregulated Ortholog(s) have endopolyphosphatase activity, exopolyphosphatase activity, role in polyphosphate catabolic process and fungal-type vacuole membrane, nucleus localization Protein lacking an ortholog in S. cerevisiae; transposon mutation affects filamentous growth flucytosine induced; regulated by Sef1p, Sfu1p, and Hap43p; planktonic growthinduced Putative poly(A)-binding protein; regulated by Gcn4p; induced in response to amino acid starvation (3-AT treatment); protein present in exponential and stationary growth phase yeast cultures Ortholog(s) have role in chromatin silencing at telomere, negative regulation of transcription from RNA polymerase II promoter by pheromones and chromatin accessibility complex localization Protein encoded in retrotransposon Zorro2 with similarity to retroviral endonuclease-reverse transcriptase proteins; lacks an ortholog in S. cerevisiae; transposon mutation affects filamentous growth orf19.3037 0.80 5.94 - orf19.5510 0.71 5.86 - orf19.7275 0.66 5.31 FGR24 orf19.2509.1 0.86 5.22 - orf19.1053 0.82 4.98 - orf19.4805 0.82 4.79 - orf19.6399 0.84 4.67 ATS1 orf19.6556 0.86 4.16 - Late-stage biofilm-induced gene orf19.6398 0.85 3.65 - Predicted ORF in Assemblies 19, 20 and 21; induced by nitric oxide orf19.3603 0.85 3.48 - - orf19.1715 0.82 3.33 IRO1 orf19.1797 0.73 2.95 - orf19.1789.1 0.83 2.80 LYS1 orf19.2651 0.84 2.80 CAM1-1 orf19.2296 0.70 2.35 - orf19.1610 0.75 1.98 - orf19.1893 0.81 1.90 - orf19.2555 0.82 1.87 URA5 orf19.3504 0.78 1.73 RPL23A orf19.1270 0.85 1.71 FRE3 orf19.3618 0.67 1.69 YWP1 orf19.2730 0.82 1.60 - Has domain(s) with predicted zinc ion binding activity orf19.55 0.84 1.41 - Ortholog(s) have role in cellular zinc ion homeostasis, response to toxin and plasma membrane localization orf19.2731 0.83 1.08 - - orf19.1632 0.79 0.85 - Ortholog(s) have cytosol, nucleus localization orf19.362 0.11 0.82 TLO9 orf19.7477 0.72 0.73 YRB1 orf19.6982 0.82 0.60 - Has domain(s) with predicted hydrolase activity, acting on ester bonds activity, role in GPI anchor metabolic process, intracellular protein transport and intrinsic to endoplasmic reticulum membrane localization Putative membrane protein; Hap43p-induced gene; fungal-specific (no human or murine homolog); transcription is induced by alpha pheromone in SpiderM medium Putative protein required for modification of wobble nucleosides in tRNA; induced upon adherence to polystyrene; regulated by Sef1p-, Sfu1p-, and Hap43p Putative transcription factor; role in iron utilization, pathogenesis; both IRO1 and adjacent URA3 are mutated in strain CAI4; suppresses S. cerevisiae aft1 mutant low-iron growth defect; hyphal-induced; reports differ about iron regulation Predicted ORF in Assemblies 19, 20 and 21; induced upon biofilm formation; has GutQ domain, which is associated with phosphosugar binding Saccharopine dehydrogenase (biosynthetic); enzyme of alpha-aminoadipate lysine biosynthesis pathway; functionally complements S. cerevisiae lys1 mutation; fungal-specific (no human or murine homolog) Putative translation elongation factor; genes encoding ribosomal subunits, translation factors, tRNA synthetases are downregulated upon phagocytosis by murine macrophages; Hap43p-induced gene Protein with similarity to mucins; ketoconazole-induced; fluconazoledownregulated; mRNA abundance increased in a cyr1 mutant than in wild type; colony morphology-related gene regulation by Ssn6p; late-stage biofilm-induced Putative protein of unknown function, transcription is activated in the presence of elevated CO2 Ortholog of C. parapsilosis CDC317 : CPAR2_105140 Putative orotate phosphoribosyltransferase; protein abundance is affected by URA3 expression in the CAI-4 strain background; flucytosine induced; protein level decreased in stationary phase cultures Ribosomal protein; genes encoding cytoplasmic ribosomal subunits, translation factors, tRNA synthetases are downregulated upon phagocytosis by murine macrophage; Hap43p-induced gene; sumoylation target Protein with similarity to ferric reductase Fre10p; possibly an essential gene, disruptants not obtained by UAU1 method Secreted yeast cell wall protein; possible role in dispersal in host; mutation causes increased adhesion and biofilm formation; stable propeptide; regulated by growth phase, phosphate, Ssk1p, Ssn6p, Efg1p, Efh1p, Hap43p; mRNA binds to She3p Member of a family of telomere-proximal genes of unknown function; Hap43prepressed gene Functional homolog of S. cerevisiae Yrb1p, which regulates Gsp1p GTPase activity and thereby affects nucleocytoplasmic transport and cytoskeletal dynamics; transcription is not regulated by white-opaque switching or by dimorphic transition Ortholog(s) have mitochondrion localization Putative RNA-binding protein; required for normal biofilm growth; nuclear export is facilitated by Hmt1p; transcription is upregulated in an RHE model of oral candidiasis orf19.7238 0.78 0.25 NPL3 orf19.7239 0.89 0.02 - Ortholog of C. parapsilosis CDC317 : CPAR2_700500 orf19.1676 0.80 -0.08 - Ortholog(s) have role in potassium ion transport, proton transport and integral to mitochondrial inner membrane localization orf19.4966 0.82 -0.16 - Putative mitochondrial carrier protein; transcription is alkaline upregulated orf19.2599 0.75 -0.40 CRC1 orf19.7051 0.86 -0.54 - orf19.2172 0.86 -1.56 ARA1 orf19.207 0.84 -1.74 PGA55 orf19.6796 0.86 -1.90 - orf19.4975 0.79 -2.57 HYR1 orf19.6304 0.78 -3.25 LYS5 orf19.4651 0.83 -3.36 PGA53 orf19.4297 0.77 -3.48 CKB2 orf19.4668 0.84 -3.62 - Protein not essential for viability orf19.3694 0.83 -3.99 - Ortholog of C. parapsilosis CDC317 : CPAR2_106350 orf19.150 0.83 -4.27 - orf19.4295 0.80 -5.03 - orf19.3732 0.84 -5.30 ERG25 orf19.3369 0.85 -5.48 MOH1 orf19.750 0.84 -5.84 - orf19.2059 0.73 -6.14 - orf19.4465 0.72 -6.23 - Ortholog of C. parapsilosis CDC317 : CPAR2_106880 orf19.5376 0.85 -6.40 - Predicted ORF in Assemblies 19, 20 and 21; possibly an essential gene, disruptants not obtained by UAU1 method orf19.5964 0.76 -6.57 ARF2 orf19.236 0.83 -6.75 RPL9B orf19.3803 0.83 -6.77 MNN22 orf19.1808 0.75 -6.84 - orf19.3974 0.81 -7.21 PUT2 orf19.6993 0.76 -7.44 GAP2 Mitochondrial carnitine carrier protein Ortholog(s) have cytosol, nucleus localization D-Arabinose dehydrogenase; in dehydro-D-arabinono-1,4-lactone biosynthesis; NADP+ cofactor; active toward D-arabinose, L-fucose, L-xylose, L-galactose; inhibited by metal ions, thiol group-specific reagents; induced on polystyrene adherence Putative GPI-anchored protein; adhesin-like protein; filament induced; regulated by Nrg1p, Tup1p; possibly transcriptionally regulated upon hyphal formation; mRNA binds to She3p and is localized to buds of yeast-form cells and hyphal tips Ortholog(s) have ADP-ribose diphosphatase activity, role in ribose phosphate metabolic process and cytosol, mitochondrion, nucleus localization Hyphal-induced GPI-anchored cell wall protein; macrophage-induced; repressed by neutrophil-co-culture; resistance to killing by neutrophils, azole resistance; regulated by Rfg1p, Efg1p, Nrg1p, Tup1p, Cyr1p, Bcr1p, Hap43p; biofilm-induced Phosphopantetheinyl transferase; enzyme of lysine biosynthesis; modifies, and thereby activates, Lys2p alpha-aminoadipate reductase; functional homolog of S. cerevisiae Lys5p GPI-anchored cell surface protein of unknown function; greater mRNA abundance observed in a cyr1 homozygous null mutant than in wild type Regulatory subunit of protein kinase CK2 (casein kinase II), beta' subunit; null mutants are hypersensitive to caspofungin Ortholog(s) have protein channel activity, role in protein import into mitochondrial matrix and mitochondrial inner membrane presequence translocase complex localization Has domain(s) with predicted chromatin binding activity, role in chromatin modification, regulation of transcription, DNA-dependent and nucleus localization Putative C-4 methyl sterol oxidase with role in C4-demethylation of ergosterol biosynthesis intermediates, based on similarity to S. cerevisiae Erg25p; fluconazole-induced; upregulated in biofilm and in azole-resistant strain Protein similar to S. cerevisiae Moh1p; transcription is induced in response to alpha pheromone in SpiderM medium; transcriptionally activated by Mnl1p under weak acid stress; possibly essential gene (UAU1 method); biofilm-induced Predicted ORF in Assemblies 19, 20 and 21; exogenously expressed protein is a substrate for Kex2p processing in vitro Protein with homology to magnesium-dependent endonucleases and phosphatases; regulated by Sef1p-, Sfu1p-, and Hap43p Putative ADP-ribosylation factor; mutation confers hypersensitivity to Brefeldin A Ribosomal protein L9; downregulated upon phagocytosis by murine macrophages; repressed by nitric oxide; protein levels decrease in stationary phase; Hap43p-induced Putative Golgi alpha-1,2-mannosyltransferase; regulated by Tsa1p, Tsa1Bp in minimal media at 37 deg; Hog1p-induced; induced by nitric oxide; downregulated in core stress response; planktonic growth-induced gene Putative protein of unknown function, transcription is positively regulated by Tbf1p Putative delta-1-pyrroline-5-carboxylate dehydrogenase; alkaline upregulated; protein present in exponential and stationary growth phase yeast cultures; latestage biofilm-induced gene General amino acid permease; ketoconazole, flucytosine repressed; Ssy1pdependent histidine induction; regulated by Nrg1p, Tup1p; shows colony morphology-related gene regulation by Ssn6p; biofilm- and planktonic growthinduced Putative transcription coactivator with a predicted role in sulfur amino acid metabolic process; required for yeast cell adherence to silicone substrate Predicted membrane transporter, member of the monocarboxylate porter (MCP) family, major facilitator superfamily; Hap43p-induced gene; alkaline upregulated by Rim101p; possibly an essential gene, disruptants not obtained by UAU1 method orf19.5312 0.80 -7.53 MET4 orf19.2751 0.73 -7.72 - orf19.7452 0.85 -8.47 - Ortholog of C. glabrata CBS138 : CAGL0F07271g orf19.1500 0.86 -8.73 - Ortholog of C. parapsilosis CDC317 : CPAR2_210210 orf19.3538 0.82 -9.10 FRE9 orf19.1048 0.75 -9.42 IFD6 orf19.1438 0.23 -9.97 - orf19.3696 0.77 -10.12 TOM22 orf19.638 0.83 -10.12 FDH1 orf19.5124 0.72 -10.13 RBR3 orf19.3924 0.85 -10.30 - - orf19.7034 0.85 -11.18 - - orf19.3503 0.85 -11.41 - - orf19.3610 0.66 -11.42 - orf19.5952 0.74 -11.78 - orf19.1543 0.86 -11.99 OPI1 orf19.1766 0.86 -12.31 - Secreted protein; fluconazole-induced orf19.1359 0.76 -12.50 - Ortholog of C. parapsilosis CDC317 : CPAR2_806560 orf19.3571 0.84 -13.57 - Ortholog of C. parapsilosis CDC317 : CPAR2_209420 orf19.48 0.79 -14.45 - orf19.7021 0.86 -14.98 GPH1 orf19.4673 0.83 -15.86 BMT9 orf19.7306 0.86 -15.97 - orf19.4337 0.81 -16.48 - orf19.8 0.48 -16.89 - orf19.1588 0.86 -17.03 - orf19.3133 0.82 -17.07 GUT2 orf19.681 0.71 -17.27 HAP43 orf19.5975 0.84 -17.32 TRY4 Ferric reductase; alkaline upregulated; ciclopirox olamine induced; induced by nitric oxide; regulated by Sef1p, Sfu1p, and Hap43p; Hap43p-induced gene Aldo-keto reductase family member; similar to aryl alcohol dehydrogenases; increased protein correlates with MDR1 overexpression (but not CDR1 or CDR2) in fluconazole-resistant clinical isolates; farnesol regulated; possibly essential gene Protein with homology to NADH dehydrogenase; regulated by Sef1p-, Sfu1p-, and Hap43p Putative mitochondrial import receptor subunit; shows colony morphology-related gene regulation by Ssn6p Formate dehydrogenase, oxidizes formate to produce CO2; Mig1p regulated; induced by macrophages; fluconazole-downregulated; downregulated by Efg1p under yeast, not hyphal, growth conditions; predicted cytosolic; stationary phase enriched Cell wall adhesin-like protein; expression is repressed by Rim101p; possibly an essential gene, disruptants not obtained by UAU1 method Predicted ORF in Assemblies 19, 20 and 21; upregulation correlates with clinical development of fluconazole resistance; regulated by Sef1p-, Sfu1p-, and Hap43p Sef1p-, Sfu1p-, and Hap43p-induced protein of unknown function; induced by nitric oxide independent of Yhb1p Functional homolog of S. cerevisiae Opi1p, which is a transcriptional repressor of INO1 involved in inositol biosynthesis; has leucine zipper and putative Opi1-Sin3 interaction domain; interacts with ScSin3p, but not CaSin3p Predicted ORF in Assemblies 19, 20 and 21; virulence-group-correlated expression; likely to be essential for growth, based on an insertional mutagenesis strategy; disruptants also not obtained by UAU1 method Putative glycogen phosphorylase with a role in glycogen metabolism; regulated by Ssk1p, Mig1p, Tup1p, Hap43p; fluconazole-induced; localizes to cell surface of hyphae, not yeast cells; stationary phase enriched protein Beta-mannosyltransferase, member of a 9-gene family that includes characterized genes BMT1, BMT2, BMT3, and BMT4 with roles in beta-1,2-mannosylation of cell wall phosphopeptidomannan; regulated by Sef1p-, Sfu1p-, and Hap43p Aldo-keto reductase family protein; increased transcription associated with MDR1 overexpression, benomyl or long-term fluconazole treatment; overexpression does not affect drug or oxidative stress sensitivity; stationary phase enriched Predicted membrane transporter, member of the monocarboxylate porter (MCP) family, major facilitator superfamily (MFS) Ortholog of C. parapsilosis CDC317 : CPAR2_204290 Putative mitochondrial protein of unknown function; regulated by Sef1p-, Sfu1p-, and Hap43p Glycerol-3-phosphate dehydrogenase; Plc1p-regulated; transcription is upregulated in both intermediate and mature biofilms CCAAT-binding factor-dependent transcriptional repressor required for low iron response; similar to bZIP transcription factor AP-1; HAP4L-bZIP bipartite domain; gene negatively regulated by Sfu1p; ciclopirox olamine induced Putative zinc finger DNA-binding transcription factor; fluconazole-downregulated; expression regulated during planktonic growth; expression upregulated in an ssr1 mutant; required for yeast cell adherence to silicone substrate Putative peptide:N-glycanase; gene contains variable numbers of 12-bp repeats; transcription upregulated by treatment with caspofungin, ciclopirox olamine, ketoconazole or hypoxia; gene of core caspofungin response; Hap43p-induced gene Putative transporter; mutation confers hypersensitivity to toxic ergosterol analog; fungal-specific (no human or murine homolog) orf19.6877 0.76 -17.99 PNG2 orf19.3232 0.85 -18.58 - orf19.5464 0.83 -19.04 - ORF Predicted by Annotation Working Group; overlaps SEC6/orf19.5463 orf19.4760 0.80 -19.37 - Ortholog(s) have protein-histidine N-methyltransferase activity, role in peptidylhistidine methylation, to form tele-methylhistidine and cytosol, nucleus localization orf19.6310 0.84 -21.36 - Ortholog of C. parapsilosis CDC317 : CPAR2_204000 orf19.151 0.86 -22.23 TPO5 orf19.2505 0.67 -22.56 - - orf19.257 0.85 -22.80 - Transcription is negatively regulated by Sfu1p; repressed by nitric oxide orf19.2939 0.82 -23.91 - Ortholog(s) have mitochondrion localization orf19.4963 0.86 -24.21 - orf19.3208 0.85 -24.75 DAL52 orf19.7112 0.72 -25.17 FRP2 orf19.7218 0.80 -25.48 RBE1 orf19.3222 0.81 -26.57 - orf19.5063 0.75 -26.67 COI1 orf19.4737 0.56 -26.91 TPO3 orf19.23 0.83 -27.35 RTA3 orf19.2762 0.69 -28.85 AHP1 orf19.1927 0.65 -30.15 SNM1 orf19.6257 0.43 -31.59 GLT1 orf19.4509 0.70 -32.06 - - orf19.2631 0.82 -35.29 - Hap43p-induced gene orf19.853 0.74 -35.98 SAP99 orf19.217 0.88 -37.30 - orf19.218 0.79 -38.67 BUD20 orf19.6937 0.97 -39.30 PTR2 orf19.3151 0.98 -39.37 - orf19.3607 0.99 -39.48 - Ortholog of C. parapsilosis CDC317 : CPAR2_806540 orf19.3219 0.97 -39.56 - Ortholog of C. parapsilosis CDC317 : CPAR2_302960 Putative polyamine transporter; mutation confers hypersensitivity to toxic ergosterol analog; hyphal induced; macrophage induced Ortholog(s) have role in ER-associated protein catabolic process and cytoplasm, nucleolus localization Putative allantoate permease; fungal-specific (no human or murine homolog); not essential for viability; similar to S. cerevisiae Dal5p Putative ferric reductase; alkaline upregulated by Rim101p; fluconazoledownregulated; upregulated in the presence of human neutrophils; possibly adherence-induced; regulated by Sef1p, Sfu1p, and Hap43p Cell wall protein; transcript negatively regulated by Rim101p, Efg1p, Ssn6p, alkaline conditions; signal sequence, O-glycosylation; no GPI anchor predicted; ketoconazol upregulated; biofilm-induced, regulated by Sef1p, Sfu1p, Hap43p Ortholog(s) have Golgi apparatus, endoplasmic reticulum, fungal-type vacuole membrane localization Secreted protein; ciclopirox olamine induced; regulated by Ssn6p; induced by nitric oxide independent of Yhb1p; Hap43p-induced Possible role in polyamine transport; MFS-MDR family; transcription induced by Sfu1p, regulated upon white-opaque switching; decreased expression in hyphae compared to yeast-form cells; regulated by Nrg1p; fungal-specific Similar to S. cerevisiae Rta1p (role in 7-aminocholesterol resistance) and Rsb1p (flippase); putative membrane protein; putative drug-responsive regulatory site; induced by fluphenazine, estradiol, ketoconazole, caspofungin Putative alkyl hydroperoxide reductase; immunogenic in mouse; biofilm-induced; fluconazole-induced; amphotericin B, caspofungin, alkaline downregulated; induced in core stress response; regulated by Ssk1p, Nrg1p, Tup1p, Ssn6p, Hog1p, Hap43p Protein similar to RNase MRP RNA binding protein; ciclopirox olamine induced; regulated by Sef1p-, Sfu1p-, and Hap43p Putative glutamate synthase; alkaline downregulated; transcription is downregulated in both intermediate and mature biofilms; regulated by Sef1p, Sfu1p, and Hap43p Putative secreted aspartyl protease; possible Kex2p substrate; expression greater in low iron; repressed by alpha pheromone in SpiderM medium; possibly essential gene (by UAU1 method); regulated by Sef1p, Sfu1p, and Hap43p Has domain(s) with predicted DNA binding, zinc ion binding activity and intracellular localization Protein similar to S. cerevisiae Bud20p, which affects bud site selection; transposon mutation affects filamentous growth Putative oligopeptide transporter; regulated by Stp2p and Stp3p; transcriptionally induced upon phagocytosis by macrophage; downregulated by Rim101p at pH 8; biofilm-induced Has domain(s) with predicted catalytic activity, coenzyme binding, nucleotide binding activity and role in cellular metabolic process orf19.3284 0.92 -39.60 - - orf19.7455 0.98 -40.01 - - orf19.133 0.87 -40.11 - Protein of unknown function; induced by nitric oxide independent of Yhb1p orf19.552 0.96 -40.18 - orf19.4072 0.99 -40.27 IFF6 orf19.3401 0.99 -40.37 CTA1 orf19.2808 0.98 -40.44 ZCF16 orf19.3767 0.99 -40.52 - orf19.2823 0.97 -40.54 RFG1 orf19.2060 0.94 -40.56 SOD5 orf19.1490 0.91 -40.86 MSB2 orf19.2479 0.62 -40.91 UGA4 orf19.873 0.95 -41.12 - orf19.4328 0.64 -41.25 CCC2 orf19.4599 0.96 -41.42 PHO89 orf19.3057 0.98 -41.57 - orf19.4803 0.95 -41.80 - orf19.2810 0.93 -41.84 AAP1 orf19.4897 0.99 -42.00 - orf19.6741 0.96 -42.22 - orf19.3642 0.96 -43.27 SUN41 orf19.3926 0.99 -43.52 RNY11 orf19.1084 0.77 -43.53 CDC39 orf19.4646 0.93 -43.99 UEC1 orf19.122 0.99 -44.05 CDC20 orf19.1932 0.83 -44.13 CFL4 Predicted ORF in Assemblies 19, 20 and 21; possibly an essential gene, disruptants not obtained by UAU1 method Putative GPI-anchored adhesin-like protein; opaque-specific transcription; macrophage-induced gene; Hap43p-repressed gene Protein similar to S. cerevisiae Mos10p, which affects S. cerevisiae filamentous growth; activates transcription in 1-hybrid assay in S. cerevisiae; protein levels increase under weak acid stress; nonessential Predicted zinc-finger protein; not essential for viability Ortholog(s) have signal sequence binding activity, role in protein targeting to vacuole and Golgi apparatus localization Transcriptional regulator of filamentous growth and hyphal genes; acts in Tup1pdependent and -independent pathways; binds DNA; has HMG domain; not transcriptionally regulated by oxygen or serum; not responsible for hypoxic repression Copper- and zinc-containing superoxide dismutase; protective role against oxidative stress; induced by neutrophil contact, hyphal growth, caspofungin, osmotic or oxidative stress; member of a gene family including SOD1, SOD4, SOD5, and SOD6 Adhesin-like protein; mucin family; cell wall damage sensor; N-terminal secretion signal; required for Cek1p phosphorylation in response to cell wall stress; Rim101p-repressed; activation releases extracellular domain into medium Putative gamma-aminobutyric acid/polyamine permease; nitrogen catabolite repressed gene, induced in absence of preferred N sources; transcriptionally induced upon phagocytosis by macrophage; gene regulation by nitrogen source requires Gat1p Copper-transporting P-type ATPase of Golgi; required for wild-type iron assimilation (indirect effect via Fet3p); induced by iron starvation, ciclopirox olamine; caspofungin repressed; not required for virulence in mouse systemic infection Putative phosphate permease; expression is regulated upon white-opaque switching; alkaline upregulated by Rim101p; induced upon biofilm formation; possibly adherence-induced Ortholog(s) have role in mitochondrial genome maintenance and endoplasmic reticulum localization Putative amino acid permease; fungal-specific (no human or murine homolog); possibly an essential gene, disruptants not obtained by UAU1 method Ortholog(s) have phosphatidylinositol transporter activity, role in phospholipid transport and cortical endoplasmic reticulum, cytosol, microsome localization Predicted ORF in Assemblies 19, 20 and 21; regulated by Nrg1p, Tup1p Cell wall glycosidase involved in biofilm formation and cell separation; possibly secreted; hypoxia and hyphal induced; caspofungin repressed; Efg1p, Cph1p regulated; O-glycosylation, potential Kex2p substrate; 9 5' E-boxes; 5'-UTR intron; Ortholog(s) have endoribonuclease activity, role in RNA catabolic process, apoptotic process, ell morphogenesis and cytosol, extracellular region, vacuole localization Protein similar to S. cerevisiae Cdc39p, which is part of the CCR4-NOT transcription regulatory complex; transposon mutation affects filamentous growth Protein required for damage to oral epithelial cells and for normal hyphal growth and stress resistance; transcription induced on contact with vascular endothelial cells; not highly conserved Protein similar to S. cerevisiae Cdc20p; induced under Cdc5p depletion; member of conserved Mcm1p regulon; mRNA expression peaks at cell-cycle G2/M phase; mRNA binds to She3p and is localized to buds of yeast-form cells and hyphal tips C-terminus similar to ferric reductases; expression high in low iron; transcription negatively regulated by Sfu1p; ciclopirox olamine induced; shows colony morphology-related gene regulation by Ssn6p; Hap43p-repressed; Sef1p-regulated orf19.3087.2 0.99 -44.21 - - orf19.4145 0.91 -44.24 ZCF20 orf19.2849 0.98 -44.29 AQY1 orf19.6488 0.97 -44.30 - orf19.2379 1.00 -44.32 NOT4 orf19.2344 0.96 -44.41 ASR1 orf19.7033 0.94 -44.45 - Putative dual specificity protein phosphatase, similar to S. cerevisiae Pps1p orf19.6276 0.94 -44.76 - - orf19.410.3 0.98 -44.85 - Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, DMRL synthase); catalyzes the penultimate step in the synthesis of riboflavin; Hap43p-induced gene orf19.762 0.96 -44.94 - Has domain(s) with predicted hydrolase activity orf19.7219 0.73 -45.10 FTR1 orf19.1415 0.80 -45.65 FRE10 orf19.1483 0.96 -45.76 - orf19.2253 0.99 -45.92 - orf19.3624 0.96 -46.25 - orf19.1667 0.99 -46.66 - Hap43p-repressed gene orf19.4305.1 0.96 -46.68 - Ortholog(s) have nucleus localization orf19.1144 0.98 -46.87 - Early-stage biofilm-induced gene orf19.6024 0.98 -47.47 - - orf19.4195 0.98 -47.50 - orf19.2859 0.99 -47.55 SRP40 orf19.242 0.98 -47.65 SAP8 orf19.7348 0.98 -47.69 - - orf19.1844 0.98 -48.10 - Protein similar to ferric reductase Fre10p orf19.5954 0.93 -48.48 - orf19.6010 0.95 -49.24 CDC5 orf19.2325 0.99 -49.78 - Predicted zinc-finger protein of unknown function; regulated by Sef1p, Sfu1p, and Hap43p; Hap43p-induced Aquaporin water channel; mutant has increased resistance to osmotic shock; required for wild-type tolerance of freezing; not required for virulence in a mouse model of systemic infection; flucytosine repressed; biofilm-induced gene Ortholog of C. parapsilosis CDC317 : CPAR2_301140 Putative E3 ubiquitin-protein ligase; required for maintenance, but not induction, of hyphal development; homozygous null mutant is avirulent in mouse systemic infection despite persistence in host; repressed in rat oral candidiasis Putative heat shock protein; transcription regulated by cAMP, osmotic stress, ciclopirox olamine, ketoconazole; negatively regulated by Cyr1p, Ras1p; colony morphology-related regulation by Ssn6p; stationary phase enriched; Hap43pinduced High-affinity iron permease; required for mouse virulence, low-iron growth; iron, amphotericin B, caspofungin, ciclopirox, Hog1p, Sef1p, Sfu1p, and Hap43p regulated; complements S. cerevisiae ftr1 iron transport; Hap43p-repressed Major cell-surface ferric reductase under low-iron conditions; 7 transmembrane regions and a secretion signal predicted; repressed by Tup1p, Rim101p, Ssn6p, Hog1p, caspofungin; ciclopirox olamine induced; not required for filamentous growth Ortholog of C. parapsilosis CDC317 : CPAR2_210340 Predicted ORF in Assemblies 19, 20 and 21; expression is regulated upon whiteopaque switching; transcription is repressed in response to alpha pheromone in SpiderM medium Ortholog(s) have exoribonuclease II activity, role in mitochondrial RNA catabolic process and mitochondrial degradosome localization Has domain(s) with predicted nucleic acid binding, zinc ion binding activity and intracellular localization Putative chaperone of small nucleolar ribonucleoprotein particles; macrophage/pseudohyphal-induced Secreted aspartyl protease; regulated by growth phase, temperature, whiteopaque switch; highly expressed in opaque cells and upon deep epidermal invasion; greater expression in vaginal than oral infection; prominent role in biofilms Ortholog(s) have enzyme activator activity, role in ascospore wall assembly, meiosis I, positive regulation of protein catabolic process and anaphasepromoting complex localization Polo-like kinase; member of conserved Mcm1p regulon; depletion causes defects in spindle elongation and Cdc35p-dependent filamentation; virulence-groupcorrelated expression; likely an essential gene; disruptants not obtained by UAU1 method Ortholog(s) have RNA polymerase III type 1 promoter sequence-specific DNA binding and RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity orf19.7056 0.90 -50.16 - Putative protein of unknown function, transcription is upregulated in clinical isolates from HIV+ patients with oral candidiasis; regulated by Sef1p-, Sfu1p-, and Hap43p orf19.4149 0.98 -50.33 - Predicted ORF in Assemblies 19, 20 and 21; overlaps orf19.4149.1 orf19.5802 0.94 -50.53 - orf19.1868 0.96 -50.77 RNR22 orf19.2926 0.99 -51.04 - orf19.2119 0.98 -51.16 NDT80 orf19.2378 0.99 -52.11 - orf19.2552 0.99 -52.12 - orf19.2863 0.97 -52.42 - orf19.2176 0.97 -54.83 IFM3 orf19.5013 0.98 -54.85 AGM1 orf19.7212 0.97 -55.20 - orf19.4269 0.68 -56.59 - orf19.7148 0.96 -56.84 TPO2 orf19.1939 0.99 -57.25 - orf19.22 0.62 -57.44 - orf19.5779 0.98 -57.75 RNR1 orf19.5648 0.97 -57.90 - Ortholog of C. parapsilosis CDC317 : CPAR2_402190 orf19.2181 0.99 -59.57 - ORF Predicted by Annotation Working Group; overlaps orf19.2180 orf19.4304 0.81 -61.93 GAP1 orf19.4795 0.98 -62.30 - orf19.5228 0.96 -65.22 RIB3 orf19.2179 0.93 -65.30 SIT1 orf19.4146 0.98 -67.87 SMD3 orf19.2103 0.99 -68.38 - orf19.4215 0.96 -68.48 FET34 orf19.3400 0.99 -68.76 COQ3 orf19.4690 0.93 -70.88 - orf19.6659 0.93 -72.24 GAP6 Ortholog(s) have role in maturation of SSU-rRNA and cytoplasm, nucleus localization Putative ribonucleoside diphosphate reductase; shows colony morphology-related gene regulation by Ssn6p; RNA abundance regulated by tyrosol and cell density; Hap43p-repressed gene; biofilm- and planktonic growth-induced Ortholog(s) have 5'-3' exonuclease activity, damaged DNA binding activity, role in DNA repair and nucleus localization Activator of CDR1 induction by antifungal drugs; required for wild-type drug resistance; transcriptionally induced upon antifungal drug treatment; similar to S. cerevisiae Ndt80p, which is a meiosis-specific transcriptional regulator Ortholog(s) have role in vacuolar proton-transporting V-type ATPase complex assembly and integral to endoplasmic reticulum membrane localization Protein similar to S. cerevisiae Pmr1p; amphotericin B induced; previously merged with orf19.2553; unmerged from orf19.2553 in a revision of Assembly 21 Ortholog of C. parapsilosis CDC317 : CPAR2_802560 Hap43p-repressed gene; Plc1p-regulated; overlaps orf19.2177 Phosphoacetylglucosamine mutase (N-acetylglucosamine-phosphate mutase); enzyme of UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis Phosphorylated protein; homozygous transposon insertion causes decreased colony wrinkling in filamentous growth-inducing conditions, but does not block hyphal formation in liquid media; Hap43p-repressed gene; planktonic growth-induced gene Has domain(s) with predicted ATP binding, protein kinase activity and role in protein phosphorylation Putative polyamine transport protein; fungal-specific (no human or murine homolog) Ortholog of C. parapsilosis CDC317 : CPAR2_303320 Protein with homology to peroxisomal membrane proteins; Sef1p-, Sfu1p-, and Hap43p-regulated gene Ribonucleotide reductase large subunit; expression greater in low iron; transposon mutation affects filamentous growth; farnesol upregulated in biofilm; regulated by cell cycle, tyrosol, cell density; regulated by Sef1p, Sfu1p, and Hap43p Amino acid permease; antigenic in human/mouse; 10-12 transmembrane regions; regulated by nitrogen source; alkaline, GlcNAc, phagocytosis, biofilm induced; not required for virulence in mouse systemic infection; fungal-specific Sef1p-, Sfu1p-, and Hap43p regulated gene 3,4-Dihydroxy-2-butanone 4-phosphate synthase; homodimeric enzyme of riboflavin biosynthesis; converts ribulose 5-phosphate to L-3,4-dihydroxy-2butanone 4-phosphate; transcription regulated on yeast-hyphal switch, macrophage interaction Transporter of ferrichrome siderophores, not ferrioxamine B; required for wild-type invasion of human epithelial cells in vitro, but not for wild-type systemic infection in mice; transcription regulated by iron, Sfu1p, SRfg1p, Tup1p, Hap43p Putative core snRNP protein; induced upon adherence to polystyrene Putative multicopper ferroxidase; expression greater in low iron and reduced in a fluconazole-resistant isolate; downregulated by Sfu1p, Hog1p; alkaline upregulated by Rim101p; ciclopirox olamine, ketoconazole and hypoxia induced Protein with a predicted role in coenzyme Q biosynthesis; transcriptionally induced by interaction with macrophages; possibly an essential gene, disruptants not obtained by UAU1 method Biofilm-induced gene; induced by nitric oxide independent of Yhb1p Broad-specificity amino acid permease; Plc1p-regulated; Gcn4p-regulated; fungalspecific (no human or murine homolog) orf19.2061 0.55 -75.55 - orf19.4555 0.76 -75.56 ALS4 orf19.1930 0.76 -99.94 CFL5 orf19.4270 0.90 -114.49 - orf19.7114 0.92 -114.60 CSA1 orf19.2062 0.44 -117.66 SOD4 orf19.5634 0.64 -120.37 FRP1 orf19.1264 0.75 -131.87 CFL2 orf19.5635 0.60 -156.53 PGA7 orf19.5633 0.93 -157.74 - orf19.5227 0.95 -163.86 - orf19.5636 0.89 -251.78 RBT5 Protein not essential for viability ALS family protein; role in adhesion and wild-type germ tube induction; growth and temperature regulated; expressed during infection of human buccal epithelial cells; down-regulated upon vaginal contact; putative GPI-anchored Ferric reductase; expression greater in low iron; negatively regulated by Sfu1p; ciclopirox olamine, flucytosine induced; amphotericin B repressed; transcription is positively regulated by Tbf1p, Hap43p; Sfu1p-regulated Putative mannosyltransferase; regulated by Sef1p-, Sfu1p-, and Hap43p Surface antigen on elongating hyphae and buds; no obvious hyphal defects in mutant; strain variation in repeat domain number; upregulated in filaments; alkaline upregulated by Rim101p; ciclopirox induced; Efg1p-, Cph1p, Hap43pregulated Copper- and zinc-containing superoxide dismutase; role in response to host innate immune ROS;regulated on white-opaque switching; ciclopirox olamine induced; caspofungin repressed; family includes SOD1, SOD4, SOD5, SOD6; yeast-associated Ferric reductase; alkaline-induced by Rim101p; iron-chelation-induced by CCAATbinding factor;fluconazole-downregulated; ciclopirox-, biofilm-, hypoxia-, Hap43pinduced; colony morphology-related regulation by Ssn6p; 2 CCAAT box motifs Putative oxidoreductase, iron utilization; regulated by Sfu1p, Sef1p, Hap43p, Nrg1p, Tup1p, Rim101p;alkaline, low iron, fluphenazine, ciclopirox olamine, flucytosine, fluconazole, biofilm induced; caspofungin, amphotericin B repressed Hyphal surface antigen precursor; possible GPI-anchor; induced by ciclopirox olamine, ketoconazole,or by Rim101p at pH 8; regulated during biofilm and planktonic growth; cell wall regeneration-induced; Hap43p-, fluconazole, biofilminduced Late-stage biofilm-induced gene Ortholog(s) have role in chaperone-mediated protein complex assembly, nucleartranscribed mRNA catabolic process, non-stop decay, proteasome assembly and intracellular localization GPI-anchored cell wall protein involved in hemoglobin utilization; regulated by Rfg1p, Rim101p, Tbf1p,iron; repressed by Sfu1p, Hog1p, Tup1p; induced by serum, alkaline pH, ketoconazole, ciclopirox olamine, geldamycin, Hap43p, biofilm Orfs are sorted by ∆OCC scores. Positive and negative ∆OCC scores reflect potential activation and repression at the chromatin level, respectively, under iron replete conditions. Gene descriptions have been sourced from Candida Genome database (http://www.candidagenome.org/). Table S3: Hyphae-related genes affected by iron at the chromatin level. ORF CORR ∆OCC NAME orf19.6645^ 0.89 89.05 HMO1 orf19.2107.1^ 0.97 75.30 STF2 orf19.1461 0.98 67.83 - orf19.2154 0.99 62.51 HXK1 N-acetylglucosamine (GlcNAc) kinase; required for wild-type hyphal growth orf19.7498^ 0.76 60.35 LEU1 3-isopropylmalate dehydratase; decreased expression in hyphae vs yeast cells orf19.3818 0.98 54.41 GOA1 orf19.1220 0.97 54.15 RVS167 orf19.637^ 0.48 54.02 SDH2 orf19.583 0.99 53.95 - orf19.2803^ 0.91 53.93 HEM13 orf19.6127 0.99 52.98 LPD1 Putative dihydrolipoamide dehydrogenase; soluble in hyphae orf19.3802 0.94 52.41 PMT6 Protein mannosyltransferase role in hyphal growth signaling orf19.5563 0.98 52.40 RNH1 Putative Ribonuclease H (RNAse H); hyphal-induced orf19.5076 0.99 52.37 PFY1 Profilin, hyphal-induced orf19.2157 0.99 51.95 DAC1 N-acetylglucosamine-6-phosphate (GlcNAcP) deacetylase; required for wildtype hyphal growth orf19.2075 0.99 50.38 DFG5 N-linked mannoprotein of cell wall and membrane; role in hyphal growth orf19.4433 0.97 50.04 CPH1 Transcription factor required for mating and hyphal growth on solid media orf19.6854 0.97 49.59 ATP1 ATP synthase alpha subunit; at hyphal surface, not yeast orf19.5493 0.89 49.46 GSP1 Small RAN G-protein; not transcriptionally regulated by white-opaque or yeasthyphal switching orf19.5806^ 0.97 48.32 ALD5 NAD-aldehyde dehydrogenase; decreased expression hyphae orf19.7544 0.98 48.31 TLO1 orf19.3599 0.97 47.97 TIF4631 orf19.6621 0.96 47.19 MHP1 orf19.1773 0.99 45.78 RAP1 orf19.548 0.99 44.74 CDC10 orf19.3707^ 0.93 44.36 YHB1 orf19.550 0.99 44.08 PDX3 orf19.796 0.96 43.14 HYM1 orf19.449 0.92 42.97 - orf19.3176 0.96 42.81 RIM21 orf19.6626 0.99 41.38 - orf19.3182^ 0.95 40.69 GIS2 orf19.4448 0.96 40.47 SOG2 orf19.6091 0.96 40.02 RIM8 DESCRIPTION Transcription factor; expression repressed in hyphae relative to yeast-form cells Protein involved in ATP biosynthesis; decreased expression in hyphae vs yeast-form cells Predicted ORF in Assemblies 19, 20 and 21; possibly transcriptionally regulated upon hyphal formation Protein required for respiratory growth, resistance to oxidants, chlamydospore formation, hyphal growth under some conditions SH3-domain- and BAR domain-containing protein involved in endocytosis; null mutant exhibits defects in hyphal growth Succinate dehydrogenase, Fe-S subunit; localizes to surface of yeast cells, but not hyphae Putative indoleamine 2,3-dioxygenase (IDO); enzyme inhibition by 1-methylD,L-tryptophan causes increased hyphal growth; expressed in yeast cells and hyphae Coproporphyrinogen III oxidase; localizes to yeast cell surface, not hyphae Member of a family of telomere-proximal genes of unknown function; hyphalinduced expression Putative translation initiation factor eIF4G; overexpression causes hyperfilamentation; hyphal- induced Protein similar to S. cerevisiae Mhp1p, which is involved in microtubule stabilization; possibly transcriptionally regulated upon hyphal formation Transcriptional regulator that binds telomeres and regulatory sequences in DNA; represses hyphal growth under yeast-favoring conditions Septin, required for wild-type cell, hyphal, or chlamydospore morphology Nitric oxide dioxygenase; hyphal downregulated Pyridoxamine-phosphate oxidase; transcription regulated by yeast-hyphal switch Mo25 family domain protein of RAM cell wall integrity signaling network; required for hyphal growth Putative phosphatidyl synthase; transcription reduced upon yeast-hyphal switch Plasma membrane pH-sensor; required for for alkaline pH-induced hyphal growth Predicted ORF in Assemblies 19, 20 and 21; possibly transcriptionally regulated upon hyphal formation Putative transcription factor; expression reduced upon yeast-hyphal switch Leucine-rich-repeat domain protein of RAM cell wall integrity signaling network; required for hyphal growth beta-arrestin-like protein involved in pH response; required for alkaline pHinduced hyphal growth NAD-linked glyceraldehyde-3-phosphate dehydrogenase; at yeast and hyphal surface Biofilm- and planktonic growth-induced gene; transcription regulated upon yeast-hyphal switch Putative carboxypeptidase y inhibitor; transcription is regulated upon yeasthyphal switch orf19.6814 0.94 39.68 TDH3 orf19.5334 0.82 36.84 - orf19.1974 0.77 36.14 TFS1 orf19.4318^ 0.83 34.37 MIG1 Transcriptional repressor; hyphal downregulated orf19.1354^ 0.83 30.16 UCF1 Hap43p-repressed gene; transcription decreased upon yeast-hyphal switch orf19.7001 0.85 26.17 YCK2 Plasma membrane protein similar to S. cerevisiae casein kinase I, Yck2p; null mutant has defect in hyphal branching orf19.2871 0.85 24.06 SDH12 orf19.3967^ 0.78 21.32 PFK1 orf19.2531^ 0.85 15.04 CSP37 orf19.6798^ 0.63 11.48 SSN6 orf19.3426^ 0.86 9.44 ANB1 orf19.6638^ 0.80 8.84 PTC4 Phosphatase; decreased expression in hyphae compared to yeast-form cells orf19.1715 0.82 3.33 IRO1 Putative transcription factor; hyphal-induced orf19.207 0.84 -1.74 PGA55 Putative GPI-anchored protein; possibly transcriptionally regulated upon hyphal formation; mRNA binds to She3p and is localized to buds of yeast-form cells and hyphal tips orf19.4975 0.79 -2.57 HYR1 Hyphal-induced GPI-anchored cell wall protein orf19.638 0.83 -10.12 FDH1 orf19.7021 0.86 -14.98 GPH1 orf19.151 0.86 -22.23 TPO5 Putative polyamine transporter; hyphal induced orf19.4737 0.56 -26.91 TPO3 Possible role in polyamine transport decreased expression in hyphae compared to yeast-form cells orf19.2823* 0.97 -40.54 RFG1 Transcriptional regulator of filamentous growth and hyphal genes orf19.2060* 0.94 -40.56 SOD5 Copper- and zinc-containing superoxide dismutase; induced by hyphal growth orf19.3642* 0.96 -43.27 SUN41 orf19.4646 0.93 -43.99 UEC1 orf19.122 0.99 -44.05 CDC20 orf19.2379* 1.00 -44.32 NOT4 orf19.7212* 0.97 -55.20 - orf19.5228* 0.96 -65.22 RIB3 orf19.7114 0.92 -114.60 CSA1 orf19.5635* 0.60 -156.53 PGA7 Succinate dehydrogenase; soluble protein in hyphae Phosphofructokinase alpha subunit activity reduced on hyphal induction Hyphal cell wall protein; expressed in yeast and hyphae; hyphal downregulated Functional homolog of S. cerevisiae Cyc8p/Ssn6p hyphal growth regulator repressed during hyphal growth Translation initiation factor eIF-5A; decreased expression in hyphae vs yeast cells Formate dehydrogenase; downregulated by Efg1p under yeast, not hyphal, growth conditions Putative glycogen phosphorylase with a role in glycogen metabolism ; localizes to cell surface of hyphae, not yeast cells Cell wall glycosidase involved in biofilm formation and cell separation; hyphal induced Protein required for damage to oral epithelial cells and for normal hyphal growth and stress resistance Protein similar to S. cerevisiae Cdc20p;mRNA binds to She3p and is localized to buds of yeast-form cells and hyphal tips Putative E3 ubiquitin-protein ligase; required for maintenance, but not induction, of hyphal development Phosphorylated protein; homozygous transposon insertion causes decreased colony wrinkling in filamentous growth-inducing conditions, but does not block hyphal formation in liquid media 3,4-Dihydroxy-2-butanone 4-phosphate synthase; transcription regulated on yeast-hyphal switch Surface antigen on elongating hyphae and buds; no obvious hyphal defects in mutant Hyphal surface antigen precursor Orfs are sorted by ∆OCC scores. Positive ∆OCC scores reflecting activation at the chromatin level under iron replete conditions are highlighted in bold text. Orfs associated with hyphae formation or yeast-hyphae transition are marked as *, while orfs with decreased expression in hyphae are marked as ^.
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