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a
TM0105 TA1R
5/1833 3/1833
TB7R TC3R
1/1833 0/1833
TB2R TC3F
0/1833 0/1833
TB21R
b
G1
M2
TA3F
50kb
TB2R
G3
G2
M1
G5
G4
M3
M4
M6
M5
inverted region
0cM
d
TC3F
(393)(84)
389 75 609
183
REV
FIP
GAG
MYB
CAS
PP
OXI
IF2
PME
RHZF
PUM
EP
PUM
TB2R
RE
LRRPK
26SP
TC3R
ANK
c
10kb
TB2R
(+365)
60 204 249 99
195 93
264 399
1kb
e
B22Not
2/409
c16
P1301
0/292
TM0885
0/173
c8
0/798
10kb
M7
M8
G7
M9
G8
575 69 621 183 60204
249 99
PPR
LRRPK
P1301
DNABP
f
POL
WD40
NifU
P1301
10kb
(77)
195 93 267 607
1kb
Supplementary Figure 1
2004-02-15137B/Kawasaki /Suppl.Fig.1ppt
Supplementary Figure 1 Positional cloning of CASTOR and POLLUX genes.
a, Genetic map around the CASTOR locus. Positions of flanking markers are
indicated together with the number of recombinant plants/total number of
mapping individuals. b, Physical map of the CASTOR locus. Designations of
large insert clones originating from parental ecotype B-129 are; G1, BAC1561E; G2, BAC124-7B; G3, BAC324-1D; G4, BAC104-3F; G5, BAC33-3E; and
from parental ecotype MG-20: M1, LjT17M09; M2, LjT62O06; M3, LjT02K14;
M4, LjT45I15; M5, LjT20F11 and M6, LjT46G19. An inverted region of 145kb
between the genomes of B-129 and MG-20 was detected, which is probably
responsible for the observed suppression of recombination around the
CASTOR locus (0 cM). BAC end markers within the inverted region are; open
oval, TB21R; closed oval, TA3F; open rectangle, TB2R. The TB7R marker
located in the north side delimits CASTOR to ~240kb. c, Features and
candidate genes predicted within the target region. LRRPK, leucine-rich
repeat receptor kinase; 26SP, 26S proteasome regulatory subunit 7; RE,
retro-element; EP, Avr9/Cf-9 elicited protein; PUM, pumilio-family RNAbinding protein; RHZF, Ring H2 zinc finger protein; PME, pectin methyl
esterase; IF2, initiation factor 2 subunit; OXI, oxido-reductase; PP,
polyprotein; MYB, myb family protein; GAG, gag-pol polyprotein; FIP; VirFinteracting protein FIP1; ANK, ankyrin-like protein; REV; reverse
transcriptase; unlabelled, hypothetical protein. d, Exon-intron structure of
CASTOR. Exons are indicated by upper boxes and numbers indicate their
length in nucleotides. Introns are represented by lower triangles. Several
alternative-splice variants were identified by cDNA-sequencing; a 4-nt
insertion at the 3’ end of the 1st exon, and a 9-nt insertion at the 5’ end of the
2nd exon. The resulting exon sizes are shown in brackets. Also, the 7 th intron
of 365 nucleotides (+365) was retained in 15 out of 17 cDNA clones, leading
of a premature stop codon. e-f Genomic region around the POLLUX gene: e,
Genetic and physical map of the POLLUX locus. Positions of the flanking
markers and the number of recombinant plants in the mapping populations
are indicated. Abbreviations G6, G7, M7, M8 and M9 refer to large insert
clones BAC131-3b, BAC45-6C, LjT39N07, LjB22b22 and LjT45B09,
respectively. Genes predicted on the cosegregating TAC clone LjT45B09;
WD40, WD40 repeat protein; NifU, putatively involved in Fe-S cluster
synthesis; PPR, pentatricopeptide repeat protein; DNABP, DNA binding
protein; LRRPK, leucine-rich repeat receptor kinase; unlabelled, hypothetical
protein. f, Exon-intron structure of POLLUX. One alternative splice site was
detected at the beginning of the 10th exon resulted in a 16-bp deletion.