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Chloroplasts in Bundle sheath and Chloroplasts in Mesophyll cells are Different:
chloroplasts in C4 mesophyll cells and bundle sheath cells are specialized for each stage of
photosynthesis. In mesophyll cells, chloroplasts are specialized for the light reactions, so they lack
rubisco, and have normal grana and thylakoids, which they use to make ATP and NADPH, as well as
oxygen. They store CO2 in a four-carbon compound, which is why the process is called "C4
photosynthesis". The four-carbon compound is then transported to the bundle sheath chloroplasts,
where it drops off CO2 and returns to the mesophyll.
Bundle sheath chloroplasts do not carry out the light reactions, preventing oxygen from building up in
them and disrupting rubisco activity. Because of this, they lack thylakoids organized into grana
stacks—though bundle sheath chloroplasts still have free-floating thylakoids in the stroma where they
still carry out cyclic electron flow, a light-driven method of synthesizing ATP to power the Calvin cycle
without generating oxygen. They lack photosystem II, and only have photosystem I—the only protein
complex needed for cyclic electron flow. Because the job of bundle sheath chloroplasts is to carry out
the Calvin cycle and make sugar, they often contain large starch grains.