Download Part I---Bioenergetics and metabolism

Syllabus of Biochemistry I
Lecture 1---Biochemistry and the genomic Revolution
 DNA illustrates the relation between form and function
 Biochemical unity underlies biological diversity
 Chemical bonds in biochemistry
 Biochemistry and human biology
Lecture 2---Biochemical evolution
 Key organic molecules used in living system
 Evolution requires reproduction, variation, and selective pressure
 Energy transformations are necessary to sustain living system
 Cells can respond to changes in their environment
Lecture 3---Protein structure and function
 Proteins are built from a repertoire of 20 amino acids
 Primary,
secondary , tertiary , and quaternary structure of proteins
 The amino acid sequence of a protein determines its three dimensional structure
Lecture 4---Exploring proteins (1)
 The purification of the proteins is the first essential step towards understanding
their functions
 Techniques used in protein purification
Lecture 5---Exploring proteins (2)
 Techniques used in protein purification (continued)
 Determine the amino acid sequence of a protein
 Synthesis of peptides
 Determine the three dimensional structures of proteins by NMR and X-ray
crystallography
Lecture 6---Enzymes (1)
 Enzymes are powerful and highly specific catalysts
 Free energy is a useful thermodynamic function for understanding enzymes
 The Michaelis-Menten model accounts for kinetic properties of many enzymes
Lecture 7---Enzymes (2)
 Enzymes can be inhibited by specific molecules
 Vitamins are often precursors of coenzymes
 Basic catalytic principle used by many enzymes
 Proteases: Facilitating a difficult reaction
Lecture 8---Enzyme (3)
 Carbonic anhydrases: Making a fast reaction center
 Restriction enzymes: Performing highly specific DNA cleavage reactions
 Nucleoside monophosphate kinase
Lecture 9---Carbohydrates
 Monosaccharides, disaccharides, and polysaccharides
 Glycoconjugates: Proteoglycans, glycoproteins, and glycolipids
 Carbohydrates as informational molecules: The sugar code
Lecture 10---Nucleotides and nucleic acids
 Chemical composition and structure of nucleotides
 Discovery as genetic materials
 Structure of DNA and RNA
 Mutation of DNA
 DNA sequencing technology
Lecture 11---Lipids
 General properties of lipids
 Storage lipids
 Lipids as structural elements
 Lipids as functional molecules
Lecture 12---Biological membranes and transport
 The composition and architecture of membranes
 Membrane dynamics
 Solute transport across membranes
Lecture 13---Biosignaling (1)
 General introduction of biosignaling
 Receptor enzymes (Insulin signaling pathway)
 Receptors with no intrinsic enzyme activity
Lecture 14---Biosignaling (2)
 G protein-coupled signaling pathway
 Second messenger
 Signaling mechanism steroid hormone
 Regulation of cell cycle
 Oncogenes, tumor suppressors, and aptopsis
Lecture 15---Biosignaling (3)
 Signaling in bacterial system
 General introduction of plant cell signaling
 Signaling pathway of ethylene