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wastewater thyroid issues breast cancer urine “superbugs” stream biofilms streams soil endocrine disruption blood benthic bacteria cardiac muscle dysfunction Understanding the Environmental Implications of Triclosan Degradation Shivani S.R. Pandey Mount Hebron High School Johns Hopkins Applied Physics Lab Dr. Charles Young • Additional background • Research focus and experiments • Future plans Outline 1 “down the drain” consumer products hospitals food processing 2 3 wastewater treatment plants aquatic bodies biodegradation Chesapeake Bay Atlantic Ocean Into the Environment 14 unique colonies 9 distinct liquid media tryptic soy broth (TSB) minimal salts medium (MSM) + TCS TSB + 5mg/L TCS MSM + glucose TSB + 50mg/L TCS MSM + sodium acetate TSB + 100mg/L TCS MSM + TCS + glucose MSM + TCS + sodium acetate Experiments Absorbance (nm) 1 8 15 22 29 36 43 50 57 64 71 78 85 92 99 106 113 120 127 134 141 148 155 162 169 176 183 190 197 204 211 218 225 232 239 246 253 260 1 0.1 50-2 in TCS + glucose 50-2 in TCS + sodium acetate 50-4 in TCS + glucose 50-11 in TCS + glucose 50-11 in TCS + sodium acetate 0.01 Results Timepoints • Toxic byproducts • Mass spectrometry • Degradation pathway Future Research