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Study programme : Dentistry Course : Biochemistry Abbreviation : LCH/ZAB21 Course type : 15 h (lectures) 30 h (practical training) Year : 2.year, 4.semester Number of Credits : 9 Course Form : Lectures, practical training Lectures : Lecturers: Doc. RNDr. Eva Anzenbacherová, CSc. Study: Continuous Date 1 17.2.2017 2 3.3.2017 3 17.3.2017 4 31.3.2017 5 14.4.2017 6 28.4.2017 7 12.5.2017 Theme Key role of acetylcoenzyme A in metabolism. Tricarboxylic acid cycle (Citrate cycle), redox (respiratory) chain, aerobic phosphorylation. Metabolism of foreign compounds (xenobiotics). Function of the GIT, liver, kidney. Endocrine, paracrine and autocrine regulation of metabolic processes. Oral biochemistry Part I. Function of saliva, salivary diagnostics. Oral biochemistry Part II. Saliva biomarkers. Oxidative stress, radicals, reactive oxygen species, nitric oxide, antioxidants. Biochemistry of solid tissues. Osteosynthesis, osteolysis, osteoporosis. No. of Less. 2 Anzenbacherová 2 Anzenbacherová 2 Anzenbacherová 2 Anzenbacherová 2 Anzenbacherová 2 Anzenbacherová 3 Anzenbacherová Lecturer Practical training : Le Leading Lecturer: inuous Study: Doc. RNDr. Eva Anzenbacherová, CSc. Continuos Date Theme Number hrs. 1 2 14.2.2017 21.2.2017 2 2 3 28.2.2017 4 7.3.2017 5 14.3.2017 6 21.3.2017 7 28.3. 2017 8 4.4.2017 9 11.4.2017 10 18.4.2017 11 12 25.4.2017 2.5.2017 13 9.5.2017 14 15 16.5.2017 23.5.2017 Chemical literature, databases. Biochemical processes in the GIT III Optimal temperature for enzyme reaction of trypsine. Determination of creatinine in blood serum and urine. Determination of creatine kinase activity. Xenobiotics I Determination of acetylsalicylic acid. Xenobiotics II Determination of paracetamol (acetaminophen). Active compounds in oral hygiene I Sanguinarine determination in dental preparations. Active compounds in oral hygiene II Determination of fluorides in dental preparations. Biochemistry of oral cavity I Qualitative determination of inorganic compounds in saliva. Demineralization hard tissues in the oral cavity. Biochemistry of oral cavity II Qualitative determination of organic substances in saliva. Salivary protein electrophoresis on cellulose acetate membrane Biochemistry of oral cavity III Buffering capacity of saliva. Presentation of essays I Radical processes in the organism Antioxidant capacity of saliva. Determination of ascorbate. Biochemistry of oral cavity IV Determination of enzyme activities in saliva (ALT, AST, LD, ALP). Presentation of essays II Evaluation of knowledge on biochemical processes in oral cavity. Credits. Revision. The basic metabolic processes. Completed by: Requirements: Literature: 2 2 2 2 2 2 2 2 2 2 2 2 2 Course unit credit. 100% presence in practicals. Champe P. C., Harvey R.A.: Biochemistry (4th ed.), Lipincott Williams & Wilkins 2008. Devlin T.M. Textbook of biochemistry with clinical correlations (6th ed.), John Wiley & Sons, Inc. 2006.