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Collision of Particulate Matter on Selected Plants Growing On National Highway (NH-28) Faizabad, India Vipendra Kumar Singh 1, Gyan Deep Sharma 2 , Imran Ahmad 3 ,Mohd Imran Ansari 4 1 Environmental Carcinogenesis Laboratory, Food Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology Research Mahatma Gandhi Marg, P.O. Box 80, Lucknow-226001, INDIA 2 Immunotoxicology Laboratory, System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research Mahatma Gandhi Marg, P.O. Box 80, Lucknow-226001, INDIA 3 Environmental Monitoring Division Environmental Toxicology Group CSIR-Indian Institute of Toxicology Research Mahatma Gandhi Marg, P.O. Box 80, Lucknow-226001, INDIA 4 Academy of Scientific and Innovative Research, New Delhi Email: [email protected] Abstract-Air pollution introduces the toxic substances in the atmosphere which cause detrimental effects to human health and plant species. It is known fact that 60% of air pollution in small cities is caused by automobiles exhaust and heavy vehicles on highway. The effect of these pollutants is observed on perennial plants which are considers for investigation of effect of auto exhaust pollutants on highway side flora and fauna. The aim of present study to investigate the impact of accumulation of particulate matter (SPM and RSPM), Gaseous pollutant (SOx, NOx) on photosynthetic pigments (Chlorophyll a Chlorophyll b and carotenoids of seven common highway side plant species (Acacia veirbicky, Neolmarckia kadamba, Dulbergia sisso, Azadiractica indica, Magnifera indica, Ficus virens, Ficus reliagosa) growing at national highway (NH-28) between Faizabad to Ayodhya. Six samplings were performed in a period of January to June months. Further, increased concentration of particulate matter (SPM 650±24 and RSPM 412.23±20 µg/m3) and gaseous pollutant (SOx 16.52±3.2 and NOx 35.25±4.1 µg/m3) were measured with respiratory dust sampler. which was several fold greater than the normal standard and significant reduction in chlorophyll content and change in pH were recorded at the highway site plants, compared to control site (Rural area) There was a value of pollutant in controlled area (SPM 97.64±7.4 and RSPM 80.23±6 µg/m3 and SOx 9.85±1.5 and NOx 19.25±2.2 µg/m3). Change in the concentration of different photosynthetic pigments (Chlorophyll 'a' 60%, Chlorophyll 'b' 50% and carotenoids 48%). The data were analyzer using one-way ANOVA and a significant change was observed in the studied parameter. Our studies clearly indicate that the vehicular pollution reduces the concentration of photosynthetic pigments in the trees exposed to highway side pollution. Keywords: Collision, air pollution , photosynthetic pigments, ANOVA