Download Effects of virus infection on growth and photosynthesis in Vitis

Effects of virus infection on growth and photosynthesis in Vitis
vinifera L. cv Malvasia de Banyalbufar
Carámbula C1, Sampol B1, Bota J1, Cifre J1, Medrano H1, Escalona J M2
1Laboratorio
de Fisiología Vegetal, Departamento de Biología, Universidad de las Islas Baleares. Ctra. Valldemossa
Km. 7,5. 07071. Palma de Mallorca. (España).
2Conselleria d´Agricultura i Pesca. Govern de les Illes Balears. c/Foners nº 10. 07006. Palma de Mallorca. (España)
“Malvasia de Banyalbufar” is an autochthonous grapevine variety from Mallorca.
The currently existing individuals proceed from a single survival stock which was infected by Grapevine Fan Leaf Virus (GFLV),
Grapevine Leaf-Roll associated Virus (GLRaV), Grapevine Fleck Virus (GFkV) and Rugose Woody Complex (RgWC).
In a previous work (Sampol et al., 2001) meristem tip “in vitro” culture enabled to obtain some virus-free plants and some others
were still infected by GFLV and GLRaV.
OBJETIVE:
analyse the effect of virus infection on different parameters of vegetative growth and photosynthesis rate.
Main Results
Infection caused a was reduced by
44% reduction of accumulated shoot
growth
(fig. 1).
Accumulated leaf appearance rate (leaf
number per day) showed a clear reduction
from 1 leaf/day for non-infected to 0,7
leaf/day for virus infected plants (fig.2).
Figure 1:Accumulated Shoot
growth
Figure 2: Accumulated Leaf
appearance rate
Only, on exponential stage of leaf
expansion curve there are highly
significant differences between virus
infected plants and non infected ones. (fig.
3).
Photosynthesis rate showed significant
differences between infected and noninfected plants on exponential growth
stage (fig.4).
Figure 3: Leaf Expansion
Figure 4: Photosynthesis rate
The reduction of net photosynthesis
was between 20-30% in virus infected
plants with respect to non-infected ones.
Experiment
References
Bertamini, M., Muthuchelian,K., Nedunchezian,N. 2004. J. Phytopathology 152,145-152.
Borgo, M. 1991. Riv. Vitic. Enol. 2, 21-30.
Cabaleiro,C., Segura, A., García –Berríos, J.J. 1999. Am. J. Enol. Vitic. 50, 40-43.
Cifre,J., Sampol, B., Escalona, J.M., Riera, D., Raya, S., Medrano, H. 2003. XV Reunión de la Sociedad Española de Fisiología Vegetal &
VIII Congreso Hispano-Luso. Palma de Mallorca.
Clingeleffer, P.R., Krake, L.R. 1992. Am. J. Enol. Vitic. 43, 31-37.
Hoefert, L.L., Gifford Jr., E.M. 1967. Hilgardia 38, 403-426.
Hoffmann, E.L. 1984.”. Wein-Wiss 39, 16-29.
Sampol, B., Medrano, H., Juarez, J., Durán, N. 2001.. IV Congreso Ibérico de Ciencias Hortícolas. Cáceres, Mayo 2001.
Acknowledgments: This work was financed by the project: Malvasia
de Banyalbufar Recovery, Conselleria d´Agricultura i Pesca del Govern
de les Illes Balears.
Six virus infected plants (VI) and six non infected plants (NI) grown in 60 litre
containers were used in this work, all of them coming from “in vitro” culture
(Sampol et al., 2001).
Two shoot length and total number of leaves were measured on each plant
once a week during seven weeks. Length of six leaves was measured on each
plant (1st or 2nd from apex) during 18 days. Shoot length was measured with a
metal rule. Leaf elongation rate was calculated as the difference in leaf length
between 2 consecutives samplings.
The photosynthesis was determined by gas exchange using a Li-6400 (Li-Cor
Inc., Lincoln, NE, USA). Six leaves were measured by treatment (VI and NI).
Statistical analyses were performed with the SPSS for windows 10.0.