Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download (A) sickle cell perfusion (Sickle) and normal RBC
Document related concepts
Transcript
Perfusion with sickle erythrocytes up-regulates ICAM-1 andVCAM-1 gene expression in cultured human endothelial cells by Yan-Ting Shiu, Mark M. Udden, and Larry V. McIntire Blood Volume 95(10):3232-3241 May 15, 2000 ©2000 by American Society of Hematology Schematic of the flow apparatus used for exposing ECs to RBC perfusion.The endothelial monolayers were mounted in the parallel plate flow chamber by applying vacuum to hold the glass slide, gasket, and polycarbonate base together. Yan-Ting Shiu et al. Blood 2000;95:3232-3241 ©2000 by American Society of Hematology Schematic of the construction of cDNA for ICAM-1 competitors.Similar procedures were used to construct VCAM-1 and GAPDH competitors. Yan-Ting Shiu et al. Blood 2000;95:3232-3241 ©2000 by American Society of Hematology Simplified procedures of quantitative RT-PCR followed by HPLC analysis to determine ICAM-1 mRNA levels.Similar procedures were used to quantify VCAM-1 mRNA amounts. Yan-Ting Shiu et al. Blood 2000;95:3232-3241 ©2000 by American Society of Hematology ICAM-1 mRNA levels in ECs after exposure to different perfusing conditions.The time course is depicted for ICAM-1 gene expression in HUVECs after exposure to sickle cell perfusion (Sickle), normal RBC perfusion (Normal), sickle cell perfusion with cytokine ... Yan-Ting Shiu et al. Blood 2000;95:3232-3241 ©2000 by American Society of Hematology Membrane-bound ICAM-1 levels in ECs after exposure to different perfusing conditions.The time course is depicted for the cell surface expression of ICAM-1 in HUVECs after exposure to sickle cell perfusion (Sickle), normal RBC perfusion (Normal), sickle cell... Yan-Ting Shiu et al. Blood 2000;95:3232-3241 ©2000 by American Society of Hematology Soluble ICAM-1 amounts released from ECs after exposure to different perfusing conditions.The time course is depicted for the release of soluble ICAM-1 by HUVECs after exposure to sickle cell perfusion (Sickle), normal RBC perfusion (Normal), sickle cell pe... Yan-Ting Shiu et al. Blood 2000;95:3232-3241 ©2000 by American Society of Hematology VCAM-1 mRNA levels in ECs after exposure to different perfusing conditions.The time course is depicted for VCAM-1 gene expression in HUVECs after exposure to sickle cell perfusion (Sickle), normal RBC perfusion (Normal), sickle cell perfusion with cytokine ... Yan-Ting Shiu et al. Blood 2000;95:3232-3241 ©2000 by American Society of Hematology Membrane-bound VCAM-1 levels in ECs after exposure to different perfusing conditions.The time course is depicted for the cell surface expression of VCAM-1 in HUVECs after exposure to (A) sickle cell perfusion (Sickle) and normal RBC perfusion (Normal) and (... Yan-Ting Shiu et al. Blood 2000;95:3232-3241 ©2000 by American Society of Hematology Soluble VCAM-1 amounts released from ECs after exposure to different perfusing conditions.The time course is depicted for the release of soluble VCAM-1 in HUVECs after exposure to (A) sickle cell perfusion (Sickle) and normal RBC perfusion (Normal) and (B) ... Yan-Ting Shiu et al. Blood 2000;95:3232-3241 ©2000 by American Society of Hematology
