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Poster Presentation Abstract Submission Form 11 June 2015, Amsterdam During the conference (young) scientists will have the opportunity to present non-commercial posters. Abstracts can be submitted using this form only. We have limited space available. Abstracts received at [email protected] before 6 April 2015 will be considered for poster presentation. Selection will be done by the Joaquin partnership and conference organization. E-mail notification of acceptance or rejection will be sent to the submitting author no later than 1 May 2015. Further details and requirements will be given upon acceptance. Poster Title Determination of Estrogenic Activity in PM10 Air Samples from Flanders (Belgium) using the BG1 Ovarian Cancer Cell Line Authors and Affiliations K. CROES1, R. VAN DEN HEUVEL2, M. PLUSQUIN3, J. STAELENS4, B. VAN DEN BRIL4, M.S. DENISON5, T. VANDERMARKEN1, K. VAN LANGENHOVEN1, M. ELSKENS1 1 Department of Analytical, Environmental and Geo-Chemistry (AMGC), Vrije Universiteit Brussel; Pleinlaan 2, 1050 Brussels, Belgium; [email protected], [email protected], [email protected], [email protected] 2 Environmental Health and Risk, VITO; Boeretang 200, 2400 Mol, Belgium; [email protected], 3 Interuniversity Institute for Biostatistics and Statistical Bioinformatics, Hasselt University; Agoralaan 1, 3590 Diepenbeek, Belgium; [email protected] 4 Unit Air, Flemish Environment Agency (VMM); Kronenburgstraat 45, 2000 Antwerp, Belgium; [email protected], [email protected] 5 Department of Environmental Toxicology, University of California; Davis, CA 95616 USA; [email protected] Abstract (max 250 words) The abstract should state briefly and clearly the Objective, Methods, Results and Conclusions of the work. Do not include tables or figures. Endocrine disrupting chemicals (EDCs) represent a broad class of compounds, are widespread in the environment and can pose severe health effects. In this study, a novel methodology to measure the overall response of EDCs in PM10 air samples was developed and the effect of different extraction solvents was investigated. Therefore, a bioassay technique using a BG1Luc4E2 ovarian carcinoma cell line was employed to quantify the potency of the mixture of estrogenic compounds in PM10 air samples. Between April 2013 and January 2014, samples were taken in a rural, industrial and urban area in Flanders (Belgium). Estrogen disruptive potencies were reported as biologic equivalents (BEQ), expressed in pg 17β-estradiol/m³ air. Estrogenic activity was measured in more than 70% of the samples. No statistical significant differences in estrogenic activity were found between the locations, but large day-by-day variations were seen in all locations. The estrogenic activity was positively related with PM10 levels, black carbon, pyrene and fluoranthene, while a significant negative correlation with air temperature was found. Furthermore, the estrogenic potency was generally higher when a polar extraction solvent was used (ethanol or acetonitrile) instead of a hexane/acetone (1/1) mixture. This study shows that the in vitro bioassay is a suitable tool to monitor estrogenic disruption in air samples. However, it seems that changes in sample pre-treatment methods can significantly influence the observed potencies and that a harmonized approach for sampling, extraction and in vitro measurement is thus necessary. Contact Name and Position Organisation Vrije Universiteit Brussel (VUB) Address Pleinlaan 2 City Brussels Postal Code 1050 Country Belgium Telephone +32 2 629 32 93 E-mail [email protected]