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A human Mix-like homeobox gene MIXL shows functional
similarity to Xenopus Mix.1
by Wei Guo, Agnes Pui-yee Chan, Hong Liang, Eric D. Wieder, Jeffrey J. Molldrem,
Laurence D. Etkin, and Lalitha Nagarajan
Blood
Volume 100(1):89-95
July 1, 2002
©2002 by American Society of Hematology
Human Mix.1-like homeobox gene MIXL. (A) The predicted ORF of MIXL. Nucleotides 1-285 were
derived from sequencing BAC CITB-HSP-C216I11.
Wei Guo et al. Blood 2002;100:89-95
©2002 by American Society of Hematology
High degree of conservation in the homeo- and acidic carboxy-terminal domains in evolution.(A)
ClustalW alignment of the 60 amino acid homeodomain sequences.
Wei Guo et al. Blood 2002;100:89-95
©2002 by American Society of Hematology
MIXL localizes to human chromosome 1q42.MIXL was localized to human chromosome 1q42 and
linked to the markers in bold by radiation hybrid mapping.
Wei Guo et al. Blood 2002;100:89-95
©2002 by American Society of Hematology
MIXL expression is restricted in normal hematopoietic tissues.(A) Human immune system
multitissue Northern blot (Clontech Laboratories) was hybridized overnight with
radiolabeledMIXL cDNA probe as described in “Materials and methods,” and the blot was
expos...
Wei Guo et al. Blood 2002;100:89-95
©2002 by American Society of Hematology
Characterization of MIXL protein expression.(A) Specificities of MIXL antibodies. 293T cells were
transfected with wild-type (WT) MIXL and mutant MIXL without the homeobox (ΔMIXL) cloned in
the vector pcDNA3.1-MycHis.
Wei Guo et al. Blood 2002;100:89-95
©2002 by American Society of Hematology
MIXL -induced expression of αT4-globin inXenopus animal caps.Synthetic mRNAs (300 pg or 1
ng RNAs) were injected into Xenopus one-cell embryos in 2 separate sets.
Wei Guo et al. Blood 2002;100:89-95
©2002 by American Society of Hematology