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RT-PCR: PRKWNK1,WNK1, T39 T40 (232 bp), Reamplifikation M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24M M 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 M 040402_Reampli_Gewebe_1_A1_T39T40_calb M = Marker 1 = Oesophagus 2 = Stomach 3 = Stomach Tumor 4 = Corpus 5 = Antrum 6 = Small Intestine 7 = Duodenum 8 = Large Intestine 9 = Large Intestine 10 = Coecum 11 = Coecum 12 = Colon 13 = Colon Tumor 14 = Rectum 15 = Rectum 16 = Cervix 17 = Endometrium 18 = Uterus 19 = Uterus Tumor 20 = Ovary 21 = Ovary Tumor 22 = Ovary Tumor 23 = Placenta 24 = Testis Bedingungen: 2% TBE 90V 1h 40min 9 µ Reampli-Probe 2 µl LB 25 = Testis Tumor 26 = Prostate gland 27 = Prostate gland 28 = Adrenal gland 29 = Adrenal gland 30 = Kidney 31 = Kidney Fetal 32 = Kidney Tumor 33 = Kidney Tumor 34 = Bladder 35 = Spleen 36 = Spleen 37 = Spleen Fetal 38 = Liver 39 = Liver 40 = Liver Fetal 41 = Pancreas 42 = Pancreas 43 = Salivary gland RT-PCR: PRKWNK1,WNK1, T39 T40 (232 bp), Reamplifikation M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 M 040402_Reampli_Gewebe_2_A1_T39T40_calb Bedingungen: 2% TBE 90V 1h 40min 9 µl Reampli-Probe 2 µl LB M = Marker 1 = Thyroid gland 2 = Thymus 3 = Thymus Fetal 4 = Tonsil 5 = Tonsil 6 = Lymph nodes Tumor 7 = Marrow 8 = Spinal Cord 9 = Brain 10 = Brain Fetal 11 = H2O 12 = Heart 13 = Heart Fetal 14 = Lung 15 = Lung 16 = Lung Tumor 17 = Breast 18 = Breast 19 = Breast Tumor 20 = Muscle 21 = Muscle 22 = Retina RT-PCR: PRKWNK1,WNK1, T41 T42 (217 bp), Reamplifikation M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 M M 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 M 040402_Reampli_Gewebe_1_A2_T41T42_calb M = Marker 1 = Oesophagus 2 = Stomach 3 = Stomach Tumor 4 = Corpus 5 = Antrum 6 = Small Intestine 7 = Duodenum 8 = Large Intestine 9 = Large Intestine 10 = Coecum 11 = Coecum 12 = Colon 13 = Colon Tumor 14 = Rectum 15 = Rectum 16 = Cervix 17 = Endometrium 18 = Uterus 19 = Uterus Tumor 20 = Ovary 21 = Ovary Tumor 22 = Ovary Tumor 23 = Placenta 24 = Testis Bedingungen: 2% TBE 90V 1h 35min 9 µ Reampli-Probe 2 µl LB 25 = Testis Tumor 26 = Prostate gland 27 = Prostate gland 28 = Adrenal gland 29 = Adrenal gland 30 = Kidney 31 = Kidney Fetal 32 = Kidney Tumor 33 = Kidney Tumor 34 = Bladder 35 = Spleen 36 = Spleen 37 = Spleen Fetal 38 = Liver 39 = Liver 40 = Liver Fetal 41 = Pancreas 42 = Pancreas 43 = Salivary gland RT-PCR: PRKWNK1,WNK1, T41 T42 (217 bp), Reamplifikation M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 M 040402_Reampli_Gewebe_2_A2_T41T42_calb Bedingungen: 2% TBE 90V 1h 40min 9 µl Reampli-Probe 2 µl LB M = Marker 1 = Thyroid gland 2 = Thymus 3 = Thymus Fetal 4 = Tonsil 5 = Tonsil 6 = Lymph nodes Tumor 7 = Marrow 8 = Spinal Cord 9 = Brain 10 = Brain Fetal 11 = H2O 12 = Heart 13 = Heart Fetal 14 = Lung 15 = Lung 16 = Lung Tumor 17 = Breast 18 = Breast 19 = Breast Tumor 20 = Muscle 21 = Muscle 22 = Retina
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