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A novel amidohydrolase (DmhA) that can hydrolyze the organophosphorus pesticide dimethoate
to dimethoate carboxylic acid and methylamine
QingChen2#, Kai Chen1#, Haiyan Ni1,Wen Zhuang3, Hongmei Wang2, Jianchun Zhu1, Qin He1, Jian
He*1
1
Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment,
Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing,
People’s Republic of China.
College of Life Sciences, Zaozhuang University, 277160, Zaozhuang, People’s Republic of China.
2
College of City and Architecture Engineering, Zaozhuang University, 277160, Zaozhuang, People’s
3
Republic of China.
1
Supporting information
Supplementary Figure 1GC-MS profile of the metabolite produced during dimethoate degradation
bystrainDC-6. A, GC spectra of the sample collected at 0 h; B, GC spectra of the extract obtained from
the culture at 4 h; C, D charged ions mass spectra for the peak at 8.68 and 9.63 min, respectively.
Supplementary Figure 2SDS-PAGE analysis of the purified DmhA. Lane M, protein markers (kDa);
lane A, total protein of Escherichia coli BL21(DE3) harbouring pET-29a(+) as control. lane B, total
protein of Escherichia coli
BL21(DE3) harbouring pET-29a-dmhA, induced by 0.5 m mol·L-1. lane C,
purified DmhA.
2
Supplementary Figure 1
Supplementary Figure 2
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