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The Cell Cell: the basic unit of living organisms HISTORY OF THE CELL History: 1665-Robert Hooke viewed thin slices of cork under a crude microscope and observed tiny hollow boxes. He called these boxes cells. 1674- Anton van Leeuwenhoek observed tiny living organisms in drops of pond water 1838- Matthias Schleiden concludes that all plants are made up of cells. 1839-Theodor Schwann concludes that all animals are made up of cells. 1855-Rudolph Virchow completes the cell theory. “ father of modern pathology” 1970- Lynn Marguilis proposes the Endosymbiont Hypothesis. The Cell Theory: 1. All living things are composed of cells. 2. Cells are the basic unit of structure and function in living things. 3. New cells are produced from existing cells. There are two types of cells: prokaryotes and eukaryotes Cell types: Prokaryote Cell Type Prokaryote Description Cells that have genetic material not contained in a nucleus. Example Bacteria Components 1. Plasma membrane: controls what enters or leaves the cell. 2. Chromosome: contains the DNA (the genetic info.) 3. Ribosomes: make proteins. Exception: Ribosomes are not true organelles because they are not enclosed in a membrane. Eukaryote Cell Type Description Example(s) Components Eukaryote Cells that contain a nucleus in which their genetic material is separated from the rest of the cell. Plants, Animals, Fungi, Protists See homework for the list of organelles Plant cells VS. Animal Cells 10/13, 10/14 Plant and animal cells differ in the following ways: Type of Cell Shape Boundary Vacuoles Chloroplasts/ Plastids Centrioles Plant Rectangular Animal Irregular or roundish Cell wall and cell Cell membrane membrane only One large Several smaller vacuole vacuoles Present Absent Absent Present How to Use a Microscope STEPS FOR USING A COMPOUND LIGHT MICROSCOPE 1. Plug the microscope in, turn on the light and place the slide on the stage. 2. Start with the scan objective (the shortest objective). The magnification is 4 X 10 = 40X. (Magnification is determined by the objective X the eyepiece.) 3. Adjust the diaphragm so the most light is allowed through. (#5) 4. Using the coarse adjustment knob (the biggest), slowly focus so that the stage moves AWAY from the objective until you just see image. You must do this step VERY SLOWLY. 5. Carefully move the slide until the image is in the center of the field of view. NOTE: Moving the slide left will move the image right and moving the slide up will move the image down etc… 6. Using the fine adjustment knob, (the smaller knob) bring the image into focus. 7. Without touching the adjustment knobs, carefully switch to the low power objective (the medium sized one). You should look to the side while switching objectives. Magnification: ____________________. NOTE: YOUR IMAGE SHOULD BE IN THE FIELD OF VIEW. IF NOT, START AGAIN AT THE BEGINNING. 8. You should only need to do some fine adjusting to bring the image into focus. 10. Without touching the adjustment knobs, carefully switch to the high power objective (the longest one). Look to the side again. Magnification: ____________________________ 11. You should only need to do some fine adjusting to bring the image into focus. WARNING: NEVER USE THE COARSE ADJUSTMENT KNOB AT HIGH POWER Cell organelles Organelle Plant cell or both function Cell (plasma) membrane Both Controls the movement of materials that enter and exit the cell Nucleus Both Nuclear Envelope Both Nucleolus Both Chromatin Both Houses the DNA of the cell, controls the functions of the cell Controls the movement of materials that enter and exit the nucleus Produces ribosomes Made out of DNA, stores the genetic information for the organism Continued cell organelles Organelle Cytoplasm Plant cell or Both both Function The site of many chemical reactions in the cell Mitochondria Both Lysosomes Both Breaks down food particles to release energy Digests excess or worn out cell parts (contains digestive enzymes) Ribosomes Both Makes proteins Endoplasmic Reticulum (Smooth) Both Serves as the cell’s transport system, site where lipids are made Continued cell organelles Cell organelle Plant cell or both function Endoplasmic Reticulum (Rough) Both Serves as the cell’s transport system, transports proteins Golgi Apparatus Both Receives, packages and labels proteins and lipids for delivery to other parts of the organism Vacuole Both Temporary storage of water, food and waste Cytoskeleton Both In plants only, also used to support heavy structures such as leaves and flowers. Acts as a scaffold to provide Continued…. Cell organelle Plant cell or both function Cilia p. 501 Animal Short, numerous, hair-like structures used in movement Flagella p. 499 Animal Longer whip-like structures, usually only a few, used in movement Cell Wall Plant Supports and protects the plant cell Centrioles Animal Used during mitosis to separate the chromosomes Continued……. Cell organelle Plant cell or both Chloroplasts Plant Plastids Plant function Transform light energy in to chemical energy and store the energy in food (sugar) Storage of starches, lipids, and pigments in plants. The Plasma membrane Function: to control the movement of substances into and out of the cell. Maintains homeostasis. Plasma Membrane selectively permeable (allows some materials to pass through while keeping other out). A.Phospholipid: Phosphate Head (hydrophilic): Water loving; Contains phosphate * POLAR Fatty Acid Tail (hydrophobic): Water fearing; Contains fatty acids: saturated are rigid and unsaturated are flexible * NONPOLAR form a bilayer :phosphate heads lined up on the outside fatty acids tails on the inside of the bilayer Small nonpolar molecules are able to pass right through the plasma membrane. Ex: O2, CO2 Entering the cell Polar molecules, ions or large molecules are not able to pass through the membrane. Ex: Na+, Cl-, H+, Ca+, H2O Polar molecules, ions and large molecules can pass through the membrane with the help of proteins. Structure B. cholesterol helps to give a little rigidity (to strengthen) to the plasma membrane Structure C. proteins can be embedded in the membrane or located outside the membrane carry out many different functions 1. protein channels -used in diffusion. 2. protein pumps-used in active transport. 3. enzymes-speeds up reactions. 4. receptor proteins-accepts certain molecules. 5. marker proteins-where the carbohydrates are attached. Structure: D. carbohydrates · attached to marker proteins · help in cell recognition so the body can distinguish between foreign substances · act like a “name tag” · called the glycocalyx THE FLUID MOSAIC MODEL · Describes the plasma membrane. · The phospholipids are able to move sideways around each other (fluid) but still stay within the membrane. · The membrane is made up of so many different molecules it is like a mosaic. · The dual-natured polarity of the phospholipids maintains the structure of the cell membrane. · The phospholipid bilayer is an example of the fact that oil (fats) and water don’t mix!!! The lipid bilayer is held together by the fact that the polar heads (hydrophilic) are attracted to water both inside and outside the cell. The fatty acid tails (hydrophobic) are repelled by the water so they “hide” inside the bilayer. Model