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Medical Research Society 2 5~ pulmonary venous pressure on pulmonary vein flow have also been studied. left ventricular filling pressure, provided the assessment is made within 36 h of the acute infarction. 16. THE VALUE OF LEFT ATRIAL VECTORCARDIOGRAPHY I N THE ASSESSMENT O F LEFT VENTRICULAR FUNCTION FOLLOWING ACUTE MYOCARDIAL INFARCTION 17. A STUDY OF RIGHT VENTRICULAR FUNCTION USING INDICES OF CONTRACTILITY DERIVED FROM PRESSURE MEASUREMENTS P. FINNEGAN and M. V. FORBES University of Birmingham, Department of Medicine, Queen Elizabeth Hospital, Birmingham The analysis of myocardial muscle mechanics in terms of the Hill analogue model led to the observation that the maximal velocity of the contractile elements (Vmax)was independent of loading (Sonnenblick, 1962, American Journal of Physiology, 202, 931). The later application of these concepts in patient studies was based on ventricular volume and pressure measurements (Hugenholtz, Ellison, Urschel, Mirsky & Sonnenblick, 1970, Circulation, 41,191). A number of investigators claimed that V,,, in the intact ventricle was also unaffected by loading and was therefore a valid index of contractility (Wolk, Keefe, Bing, Finkelstein & Levine, 1971, Journal of Clinical Zntestigation, 50, 1276; Mason, Spann & Zelis, 1970, American Journal of Cardiology, 26,248). In the present studies of right ventricular function in twenty-three patients, pressure-velocity curves were derived using the formula (dp/dt)/KPand the indices of contractility (Vmaxand V,,,,,) were calculated. The pressure-velocity curves were variable in shape and curves with single, linear descending slopes were observed in only seven patients. The other patients had pressure-velocity curves which were either biphasic or hyperbolic in form. The effects of a number of physiological variables were examined and it was demonstrated that Vmax and V,,, were not independent of the loading conditions of the ventricle. An impaired response of maximum dp/dt to inotropic stimulation was observed in patients with right ventricular failure. J. R. MUIR,G. J. WILLIAMS and G. DAVIES Department of Cardiology, University Hospital of Wales, Heath Park, Cardiff It is now established that, in the absence of lungdisease or long-standing pulmonary venous hypertension, the pulmonary arterial diastolic pressure reflects left ventricular end diastolic pressure. The measurement of pulmonary diastolic pressure by a flow guided catheter technique has, therefore, been widely used in the early detection of left ventricular failure following acute myocardial infarction. However, a non-invasive method for the measurement of left ventricular end diastolic pressure would have obvious advantages in this situation. The pulmonary arterial diastolic pressure, a timed vectorcardiogram and a standard twelve lead electrocardiogram were recorded simultaneously in thirtytwo previously well patients following an acute myocardial infarction. The recordings were made as soon as possible after the patients were free of pain (2-18 h after admission). A pulmonary arterial diastolic pressure of 15 mmHg was taken as the upper limit of normal. If the pressure was raised, the recordings were repeated twice daily until the pressure was within the normal range. Time vectorcardiograms were also obtained from sixty ‘normal’ hospitalized individuals. There was a close correlation between the initial left atrial electrical activity calculated from the timed vectorcardiogram and the pulmonary arterial diastolic pressure (correlation coefficient = 0.85 ; P c 0.001). The correlation of ‘P terminal force’ calculated from V1 of the standard ECG with the pulmonary arterial diastolic pressure did not reach significance. The vector results at the initial investigation enabled the patients to be separated into three groups corresponding to the pulmonary diastolic pressure: (a) normal (< 15 mmHg), (b) mildly elevated (16-20 mmHg), (c) severely elevated (2130 mmHg). The value for the atrial electrical activity for the sixty ‘normal’ individuals all fell within the range for normal pressure. However, the subsequent vectorcardiographic values obtained on reinvestigation of those patients with initially high pressures were poorly correlated with subsequent pressure measurements, in that left atrial electrical activity returned more slowly to normal than did the pressure. These findings indicate that analysis of the left atrial trace obtained from the timed vectorcardiogram at high gain is a valuable non-invasive indicator of 18.THE CELL MEMBRANE AND THE CARDIOSTIMULANT EFFECT O F ADRENALINE WINFREDG. NAYLER Cardiothoracic Institute, 2 Beaumont Street, London, w.1 Excitation-contraction coupling in heart muscle cells depends upon an inwards displacement of Ca2+ from the extra to the intracellular phase. Drugs which increase the peak tension developed during contraction, e.g. adrenaline, may act by increasing the amount of Caz+ which is displaced inwards in response to the arrival of the depolarizing signal. Recent studies in our laboratories have shown that adrenaline interacts with the cell membrane of cardiac muscle cells, thereby increasing its capacity to accumulate Ca2+. Possibly some of the Ca2+ which is displaced Medical Research Society inwards during the processes associated with excitation-contraction coupling is derived from superficially-located sites within or close to the cell membrane. The hypothesis that the cardiostimulant effect of adrenaline results from a cell-membrane located action is supported by the finding that its positive inotropic effect is abolished by processes which render the cell membrane freely permeable to Ca2+. 19. RELATIONSHIP BETWEEN CARBOHYDRATE AND FAT METABOLISM IN THE NORMAL AND DIABETIC DOG HEART IN vzvo S. P. ALLISON GeneraZ Hospital, Nottingham NGI 6HA Randle and his colleagues have described the relationship between glucose and fatty acid metabolism, the so-called glucose fatty acid cycle, whereby fatty acid oxidation inhibits glycolysis at the level of PFK and pyruvate dehydrogenase. This concept has derived from in uitro work and until recently had not been confirmed in vivo. Experiments were therefore carried out on anaesthetized beagle dogs weighing 8-10 kg. The coronary sinus was catheterized under radiological control and simultaneous arterial and coronary sinus blood samples were taken throughout all experiments. The elevation of free fatty acid levels from infusion of intralipid and Heparin inhibited glucose lactate and pyruvate lactate by the dog heart. The inhibition of lactate and pyruvate uptake was reversed by sodium dichloroacetate, a substance which activates pyruvate dehydrogenase. Similar data was obtained on dogs rendered diabetic with Alloxan. The significance of these results will be discussed. 20. WATER VAPOUR EXCHANGE WITHIN THE LUNG G. CUMMING Midhurst Medical Research Institute, Midhurst, Sussex Expired air contains more water vapour than inspired air, though the water content of the latter may vary within very wide limits. How far expired gas represents alveolar gas remains a matter for discussion in respect of oxygen and carbon dioxide, but there is much evidence t o suggest that expired gas is in no way representative of alveolar gas in respect of its water vapour content. There is a tacit assumption that the water vapour tension within alveoli is the vapour tension of lung water at body temperature. Since lung water contains solutes this tension will be somewhat less than the value for distilled water, but only by about 0.5 torr so that at 37°C body temperature the expected tension would be 46.5 torr. Measurements made of expired water vapour tension indicate values of less than 40 torr, equivalent to a temperature of 33°C at full saturation. Direct measurements of temperature confirm this value. In the present study water vapour tension was measured directly with a Quadropole mass spectrometer (20th Century Electronics 4806) at a mass/ charge ratio of 18. Twenty-two normal subjects were studied, their mean end tidal water vapour tension being 37.9 torr (male subjects, 38.6+ 3-3 torr (s.D.); female subjects 37.1 & 3.3 torr). At the end of a vital capacity this increased to 39.7 torr (males, 40.4_+2.8 torr; females 38.7 & 2.8 torr), and did not alter when preceded by a 10 s breath-hold. Inspired water vapour tension was then increased to about 80 torr when the end tidal tension rose from 45.4 torr in the first breath to 4 7 7 torr in the third breath. A vital capacity in the first breath yielded a value of 48.7 torr not increasing with repetition. Thirdly, the ambient air was warmed to 80°C and the end tidal and end expiratory tensions measured. These did not differ significantly from those breathing ambient air at 23°C. The explanation of these observations is thought to be as follows: during inspiration of water vapour at a tension of about 10 torr, water vapour is added so that in the alveoli it has a tension around 47 torr. Water evaporates from the fluid lining the bronchial tree and requires the latent heat of change of state and also the heat required to raise the inspirate to body temperature. The mucosal surface of the bronchi is thereby cooled and a gradient of temperature then exists down the airways from ambient in the mouth to core temperature in the alveoli. The anatomical site of this gradient i s undefined. On expiration the gas saturated at core temperature is cooled by the mucosal surface which is itself warmed by latent heat of condensation and by temperature difference. Thus the airways retain both heat and water vapour during ventilation. During breathing of air heated to 80°C the subjective sensation is of coolness, because of the large heat transfer resulting from latent heat of evaporation. The flux of water vapour occurring at an undefined part of the airways and of the order of 35 torr has important consequences for the measurement of other gases during the course of an expiration. 21. THE EFFECT OF VARIATION IN ALPHA 1ANTITRYPSIN PHENOTYPE UPON THE INCIDENCE OF RESPIRATORY ILLNESS I N A WORKING POPULATION R. B. COLE,N. C. NEVINand G. BLUNDELL Departments of Medicine and Medical Statistics, Queen’s University of Belfast, and Department of Clinical Chemistry, Belfast City Hospital An increased liability to chronic obstructive lung disease occurs in the few individuals who have inherited severe alpha I-antitrypsin (alpha 1-AT)