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1165 O’Brien Dr. Suite A Menlo Park, CA 94025 USA www.QuanDx.com 1-855-QuanDx-Care Lung Cancer ALK/ROS1/RET Testing Panel Fusion Gene Detection The ability to detect cancer specific fusion genes is important Disease Relevance: not only in cancer research, but also increasingly in clinical Non-Small Cell Lung Cancer settings to ensure that correct diagnosis is made and the optimal treatment is chosen. Current methodologies to detect such fusion genes all have distinct shortcomings. The detection of fusion genes in cancer is laborious and time consuming and usually includes fluorescence-in situ hybridization (FISH) studies and molecular analyses based on the reverse transcriptase polymerase chain reaction (RT-PCR). LC Testing Panel Description Yin-Yang probes are a novel technology for nucleic acid detection and related applications (Patent No. US 7799522). The probe itself is non-fluorescent due to the close proximity of the fluorophore and the quencher. In the presence of target the negative strand is displaced by the target and the fluorophore becomes fluorescent. The Lung Cancer ALK/ROS1/RET Testing Panel is a novel multiplex reverse transcription real-time PCR (RT-qPCR) system for simultaneous detection of 6 fusion genes including 35 fusion variants. Fusion and control gene transcripts are co-amplified in each reaction and identified by specific fluorescent probes. The LC Testing Panel provides a sensitive, specific, reliable and cost-effective tool that is easy to perform for the routine screening of lung cancer fusion transcripts. Key Features Multiplexing: simultaneous detection of 6 fusion genes with 35 fusion variants Compatible with current real-time qPCR instruments Rapid results within 2 hours Cost effective: a fraction of the cost of FISH Easy to perform for routine screening Product Specifications Fusion genes KIF5B-RET,KIF5B-ALK,CD74-ROS1,TFG-ALK,SLC34A2-ROS1,EML4-ALK Sample types FFPE, frozen, and fresh lung cancer tissue Reaction Volume 20 µL Reaction time 2 hours Internal control Sensitivity Instruments GUSB as housekeeping genes 10-4 Compatible with Bio-Rad CFX96, iCycler, ABI7500, Roche Lightcycler 480, Stratagene Mx3000P/Mx3005P, and other commonly used RT-qPCR instruments Ordering Information Cat. No. Description Size 1-200-20020 Lung Cancer ALK/ROS1/RET Testing Panel 20 reactions Research Use Only. Not intended for use in diagnostic procedures Headquarters: 1165 O’Brien Dr. Suite A Menlo Park, CA 94025 USA www.QuanDx.com Tel: 1-855-QuanDx-CARE 1-855-782-6392 Fax: 1-866-928-7828 E-Mail: [email protected] European office: Dalmore Steading East, Auldearn, Nairn, IV12 5HZ, UK Tel: +44-7832-570742 E-Mail: [email protected]