Download Lung Cancer ALK/ROS1/RET Testing Panel

1165 O’Brien Dr. Suite A
Menlo Park, CA 94025 USA
www.QuanDx.com
1-855-QuanDx-Care
Lung Cancer ALK/ROS1/RET Testing Panel
Fusion Gene Detection
The ability to detect cancer specific fusion genes is important
Disease Relevance:
not only in cancer research, but also increasingly in clinical
Non-Small Cell Lung Cancer
settings to ensure that correct diagnosis is made and the
optimal treatment is chosen. Current methodologies to detect such fusion genes all
have distinct shortcomings. The detection of fusion genes in cancer is laborious and
time consuming and usually includes fluorescence-in situ hybridization (FISH) studies
and molecular analyses based on the reverse transcriptase polymerase chain reaction
(RT-PCR).
LC Testing Panel Description
Yin-Yang probes are a novel technology for
nucleic acid detection and related
applications (Patent No. US 7799522). The
probe itself is non-fluorescent due to the
close proximity of the fluorophore and the
quencher. In the presence of target the
negative strand is displaced by the target
and the fluorophore becomes fluorescent.
The Lung Cancer ALK/ROS1/RET Testing
Panel is a novel multiplex reverse
transcription real-time PCR (RT-qPCR) system for simultaneous detection of 6 fusion
genes including 35 fusion variants. Fusion and control gene transcripts are co-amplified
in each reaction and identified by specific fluorescent probes. The LC Testing Panel
provides a sensitive, specific, reliable and cost-effective tool that is easy to perform for
the routine screening of lung cancer fusion transcripts.
Key Features

Multiplexing: simultaneous detection of 6 fusion genes with 35 fusion variants

Compatible with current real-time qPCR instruments

Rapid results within 2 hours

Cost effective: a fraction of the cost of FISH

Easy to perform for routine screening
Product Specifications
Fusion genes
KIF5B-RET,KIF5B-ALK,CD74-ROS1,TFG-ALK,SLC34A2-ROS1,EML4-ALK
Sample types
FFPE, frozen, and fresh lung cancer tissue
Reaction
Volume
20 µL
Reaction time 2 hours
Internal
control
Sensitivity
Instruments
GUSB as housekeeping genes
10-4
Compatible with Bio-Rad CFX96, iCycler, ABI7500, Roche Lightcycler 480,
Stratagene Mx3000P/Mx3005P, and other commonly used RT-qPCR
instruments
Ordering Information
Cat. No.
Description
Size
1-200-20020
Lung Cancer ALK/ROS1/RET Testing Panel
20 reactions
Research Use Only. Not intended for use in diagnostic procedures
Headquarters:
1165 O’Brien Dr. Suite A
Menlo Park, CA 94025 USA
www.QuanDx.com
Tel: 1-855-QuanDx-CARE
1-855-782-6392
Fax: 1-866-928-7828
E-Mail: [email protected]
European office:
Dalmore Steading East,
Auldearn,
Nairn, IV12 5HZ,
UK
Tel: +44-7832-570742
E-Mail: [email protected]