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FINAL REPORT – UNU BIOLAC
International Course
Title of the course
“Theoretical and Practical Course: Flow cytometry and cell sorting in
Biotechnology and Biomedicine Research”
Date and venue
March 17th - 28th, 2014
Institut Pasteur de Montevideo
Mataojo 2020, Montevideo (11400) URUGUAY
Organizers
Dra. Mariela Bollati – Fogolín – Cell Biology Unit – Institut Pasteur de
Montevideo (Uruguay)
Jean Michel Garcia PhD – HORIBA Medicals (France) – Former member of
Institut Pasteur Korea
Lothar Groebe PhD - Helmholtz Centre for Infection Research Braunschweig
(Germany)
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
Invited Speakers and teaching staff
We were able to gather an outstanding group of teachers and tutors (11) who are
worldwide leaders in their fields. Mariela Bollati (from IP Montevideo) also delivered
lectures and worked as the local teaching staff coordinator.
Name
Institution & Nationality
E-mail
Nicole Borth
Institute of Applied
Microbiology, Vienna, Austria
[email protected]
Andrew Filby
London Research Institute,
Holborn, London
[email protected]
Jean Michel Garcia
Lothar Groebe
Amadou Alpha Sall
Alvaro Luiz Bertho
Daniel Scott
María Bernarda Ganem
HORIBA Medicals, Montpellier,
France
Helmholtz Centre for Infection
Research, Hannover, France
Institut Pasteur de Dakar,
Dakar, Senegal
Oswaldo Cruz Institute –
Fiocruz, Rio de Janeiro, Brasil
Institut Pasteur Paris, Paris,
France
BD Biosciences, Buenos Aires,
Argentina
[email protected]
[email protected]
[email protected]
[email protected]
[email protected]
[email protected]
Cecilia Alonso
CURE, Rocha, Uruguay
[email protected]
Gustavo Folle
IIBCE, Monteviideo, Uruguay
[email protected]
Marcelo Comini
Institut Pasteur de Montevideo,
Uruguay
[email protected]
Furthermore, local instructor’s staff participated in the delivery of experimental
activities:
Name
Institution & Nationality
E-mail
Inés Tiscornia
Institut Pasteur de Montevideo,
Uruguay
[email protected]
Soledad Astrada
Institut Pasteur de Montevideo,
Uruguay
[email protected]
Valentina Porro
Sabina Victoria
Karen Perelmuter
Institut Pasteur de Montevideo,
Uruguay
Institut Pasteur de Montevideo,
Uruguay
Institut Pasteur de Montevideo,
Uruguay
[email protected]
[email protected]
[email protected]
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
Program
Theoretical activities (total: 30 h):
Practical activities (total: 40 h):
Topics covered during practical activities (40 h)
•
•
•
•
•
Familiarization and Operation of a flow cytometer. Cytometer performance.
Beads for calibration, counting beads, etc.
Multicolor analysis: Compensation. Dendritic Cells (DC)
Multiplexed microspheres: cytokine analysis: Supernatant from a DC culture
Methods for apoptosis detection (Annexin V/PI, Caspase 3, sub G1). Untreated and treated
cells in culture
• Analysis of DNA content and the cell cycle: cell line
• High speed cell sorting:
a- GFP recombinant cell line (enrichment, single cell deposition)
b- 2 ways sorting of PBMC
Total course hours: 70 h
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
Selected Students
Name
Surname
Institution
Country
E-mail
Fabiana
Celes
FIOCRUZ
Brazil
[email protected]
Danielle
Vasconcelos
FIOCRUZ
Brazil
[email protected]
Marina de Asis
Souza
CPqAM/FIOCRUZ
Brazil
[email protected]
Milagros
Griet
CERELA-CONICET
Argentina
[email protected]
María Eugenia
Baravalle
UNL
Argentina
[email protected]
Fernanda
Peri
UNSAM
Argentina
[email protected]
M° de los Milagros
Cámara
UNSAM
Argentina
[email protected]
Yanina
Kolling
CERELA-CONICET
Argentina
[email protected]
Victoria
Bocanegra
UNCUYO
Argentina
[email protected]
Paula
Carasi
UNLP
Argentina
[email protected]
Augusto
Bivona
UBA
Argentina
[email protected]
Aldana
Gojanovich
PUCV
Argentina
[email protected]
Brizaida Oliva
Arguelles
CIGB
Cuba
[email protected]
Yaimel
Cepero
CIM
Cuba
[email protected]
Mauricio
Vergara Castro
PUCV
Chile
[email protected]
Erika
Melchy
UNAM
Mexico
[email protected]
Katherine
Torres
UPCH
Peru
[email protected]
Ligia
Abrante
IVIC
Venezuela
[email protected]
Ricardo
Gamez
IVIC
Venezuela
[email protected]
Mona
Zidan
Tanta University
Egypt
[email protected]
Marija
Rogar
Slovenia
[email protected]
Florencia
Sardi
Uruguay
[email protected]
Patricia
Silveira
Uruguay
[email protected]
Natalia
Ríos
University of
Lyubljana
Institut Pasteur
de Montevideo
Facultad de
Medicina
Facultad de
Medicina
Uruguay
[email protected]
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
Activity Report
1. General Summary
The course was held in the city of Montevideo (Uruguay) from 17th - 28th
March 2014. Due to both the number and high quality level of the professors,
and to the fact that all participants were carefully selected among a long list of
candidates (more than 100), the course had a high international standard and
was a very successful event, from the scientific, technical and social points of
view, allowing more than 40 researchers from different countries and continents
to spent together ten working-days for fruitful discussion and interactions.
The entire course took place at Institut Pasteur de Montevideo (IPMon). More
precisely, the local organization of the course was held by Mariela Bollati-Fogolín,
(Responsible of the Cell Biology Unit, IPMon, Uruguay) together with Jean Michel
Garcia (HORIBA Medicals, France) and Lothar Groebe PhD (Helmholtz Centre for
Infection Research Braunschweig, Germany).
A group of 11 professors were invited, from Europe, Africa and America, as
specified in the table entitled Invited Speakers and teaching staff. Their travels
and other expenses were covered by the organization. In addition there were 5
practical training instructors from the Institut Pasteur de Montevideo.
2. Students Applications and Appraisal
Hundred five participants from around the world applied to perform the
course, and priority was given to 24 participants, based firstly on the excellence
of their CV or of the laboratory where they are conducting their studies, since
they do not have many opportunities to access to this kind of practical courses.
Selected participants were from Argentina, Brazil, Chile, Mexico, Cuba,
Peru, Venezuela, Slovenia, Egypt and Uruguay, mainly Master and PhD students
(please see the table entitled Selected Students). In addition, it was decided to
keep equilibrium between countries and universities in order to avoid as much as
possible the participation of more than two students belonging to the same
university or institute.
Furthermore, due to the validation of the event as a postgraduate course,
those students who asked received the credits for their Master program or PhD
curricula, meaning that this training course will be considered as a significant
element for their educational research career.
In addition, the students filled out a survey form (anonymous) at the end of
the course (the form is joined below as Annex I). Concerning the questions with
quantitative grading (from 1 to 5: 1=“poor”, 5=”excellent”), the percentage out
of the 22 surveys was:
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
Constructive suggestions and feedback were kept concerning the qualitative
comments section, and will be used as valuable input for future events.
3. Scientific Highlights
The scientific program was organized in three main activities:
- Theoretical lectures and round tables: covering from basic flow cytometry
to advance applications
- Tutorial-style classroom, computer-based exercises: dedicated to data
analysis using different software, on a basis of one computer per student
- Practical activities: sample preparation and analysis on different flow
cytometers.
The first activity was covered during the first week (30 hours). During the
round tables all professors participated and discuss with the students case
studies and propose solutions to technical problems and troubleshooting in
different situations. Since these activities were open to the public during the first
week of the course, many students and researchers from Uruguay, Argentina
and Chile who did not received fellowships to participate to the course, attended
nevertheless the lectures.
In addition, on Monday and Tuesday there was a student poster session, in
which each participant presented orally their scientific work.
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
The companies Merck-Millipore and BD Biosciences presented technical talks
and showed their latest products to the audience in exhibition booths during the
first three days of the course.
The tutorials and practical activities were developed during the second week
(40 hours). Students were divided into four groups and each group rotated
within the described activities, assuring maximized hands-on time for all the
participants.
The last day of the course was devoted to present and discuss results
obtained during the hand on training and the final evaluation of the students. All
of them succeeded and obtained rather high marks.
Also from the social point of view the meeting was very successful. All the
participants were hosted in the same hotel and were together from breakfast to
dinner, and without introducing hierarchical divisions at table, which greatly
facilitated the interactions and informal discussions.
All the participants were really grateful with the scientific level and the
excellent organization of the course, as can be seen in the evaluations of the
course they made (see students survey). This will contribute to establish much
collaboration among them.
The students and the teachers agreed in that the lectures were of high
level. For many of the participants this was the first opportunity to visit Uruguay,
and particularly Montevideo. All the participants agreed in that it was a wonderful
experience that open their minds to the existence of this small but delightful
country and their people.
4. Organization
The organization of the course was carried out by Mariela Bollati-Fogolín,
(Uruguay), Jean Michel Garcia (France) and Lothar Groebe PhD (Germany).
The main sponsors of the Course were Institut Pasteur International
Network, United Nations University (UNU BIOLAC), International Centre for
Genetic Engineering and Biotechnology (ICGEB), The World Academy of Sciences
(TWAS) and the Institut Pasteur de Montevideo.
Venue and facilities available:
The Institut Pasteur de Montevideo is located at short distance from good
housing and hotel infrastructure in Montevideo. Air plane arrivals from Europe
and USA are well covered, mostly via Brazil, but also now with some direct flights
from Paris, Madrid, Miami and New York.
Teachers and students were accommodated in the same hotel (TRYP
http://www.tryphotels.com/es/hotel-tryp-montevideoMontevideo
Hotel,
uruguay.html), which was important to foster interaction. An efficient transfer
bus ensured daily hotel/institute/hotel transport.
Theoretical lectures took place at the auditorium facilities at Institut Pasteur
de Montevideo, which has a capacity to allocate 70 persons. This room is fully
equipped for conferences and teaching activities (computer, slides projector).
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
Practical activities took place at the Education Center and the Cell Biology
Unit labs at Institut Pasteur de Montevideo. Those labs are extremely well
equipped, including a Beckman Coulter CyAn ADP analyzer, with 2 lasers and 7
color detectors, Beckman Coulter Legacy MoFlo Cell sorter, biological safety
cabinets, incubators, centrifuges. In addition, it was obtained a demo benchtop
machine from BD Biosciences (BD Accuri C6) and the students could use it during
the hand-on training.
For data analysis and processing students used a large computer room as
teaching lab, with individual computers for each of them. Projector facility allows
for direct visualization of the teachers’ tutorials. Different analysis software and
internet connection were accessible for the participants during the course.
Furthermore, internet wireless connection was available for lab top user.
Efficient secretarial and technical assistance was available, both for
organizational logistics, as well as for informatics system management. Good
previous experience in organizing theoretical and practical courses, which
resulted in a very smooth flow, with no major problems, and solving minor
obstacles fast and efficiently.
a) Academic organization
The program, invited speakers list and final selection of students were
worked out in tight coordination between the three organizers Drs M. Bollati,
Lothar Groebe and Jean Michel García.
In addition, a dedicated web page and e-mail were set early on during
organization, allowing for advertisement and efficient interaction with applicants
and invited speakers (see below).
Web:
e-mail:
http://www.pasteur.edu.uy/index.php/en/teaching/courses
[email protected]
b) Administrative organization
The Unidad de Gestión Científica (UGC) of the IP Montevideo took care of
every non-academic detail, from scheduling flight arrivals and departures to
dealing with food, lodging and travel providers. Natalia López from the UGC
executed these and other tasks with high professionalism and very efficiently.
Budgetary balance was achieved, greatly acknowledging the different
agencies that contributed to the Course: the Institut Pasteur International
Network, United Nations University (UNU BIOLAC), International Centre for
Genetic Engineering and Biotechnology (ICGEB), The World Academy of Sciences
(TWAS) and the Institut Pasteur de Montevideo.
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
Photos
Teachers and students
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
Poster session
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
Tutorials
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
Hands-on
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
Social activities
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay
5. Perspectives
To conclude, our feeling is that Flow Cytometry and Cell Sorting in
Biotechnology and Biomedicine Research Course was a very successful event. We
developed much collaboration between the speakers and the participants that
will contribute to improve the quality of the research and the exchange of
students in the region and the world.
We agree both with the speakers and the participants that it would be
necessary to establish this kind of course in the Institute or in the region more
often, every 2 or 3 years, in order to give more opportunities to regional
students to participate.
Finally, we consider that this was a successful activity taking into account
the number and quality of the applications we received. Also, from the survey all
participants agreed in the good quality of the activities taking into account time,
topics, lecturers, etc. Also professors were very satisfied with the quality of the
students allowing delivering very detailed lectures. The organization was also
positively evaluated by all participants.
Expenditures
Original documents kept at Institut Pasteur de Montevideo.
N°
1
2
3
4
5
6
Description
Bank Fee - BBVA
Lab ware – IP Montevideo (tips, tubes, pipettes, etc.)
Reagents - Life Technologies Corporation
Reagents - Macromar
Accommodation (professors and students) – Tryp Hotel
Local Transportation - Monydan
USD
30
307
816
20
7793
1032
Total
9998
This activity also received support from:
Theoretical and Practical Course: Flow Cytometry and Cell Sorting
in Biotechnology and Biomedicine Research”
Institut Pasteur de Montevideo, Montevideo, Uruguay