Download In one assay: •Analysis of 36 CF mutations and wild types •Optional

INNO-LiPA
CFTR
Innogenetics 2001
2008
©©Innogenetics
DNA line probe assay for the identification of CFTR gene mutations
Mutation CFTR dele2,3 (21kb) included
In one assay:
assay:
• Analysis
Analysisof
of36
36CF
CFmutations
mutationsand
andwild
wildtypes
types
•
• Optional
OptionalTn
Tntyping
typing
•
• Optimal
strip
layout
easier
interpretation
New strip
layout
forfor
easier
interpretation
•
• Easy
to perform
Simplified
test procedure
•
• Automatable
Automatable
•
• Objective
Objectivereading
reading
•
I N N O - L i PA C F T R 1 9 a n d C F T R 1 7 + T n U p d a t e
Extra
mutations,
Tn typing,
and and
easier
interpretation
Available
mutations,
Tn typing,
interpretation
Seven extra 36
mutations
and frequent
their wild-types
on themutations
INNO-LiPA
■ Containing
of the most
CFTR-related
in EuCFTR19
(compared
to INNO-LiPA
CFTR12):
rope
andstrip
the ACMG
recommended
mutations,
providing a good
CFTRdele2,3
711+1G➔T,
3272-26A➔G, 1898+1G➔A, I148T,
detection
rate(21kb),
for Caucasian
populations.*
3199del6, 3120+1G➔A.
■ Optional Tn typing by insertion of a marker line.
■ Optional Tn typing by insertion of a marker line.
■ Mutant probes on the top half of the strip.
■ Mutant probes on the top half of the strip.
■ Wild-type probes on the lower half of the strip.
■ Wild-type probes on the lower half of the strip.
*Available from: http://www.acmg.net/Pages/ACMG_Activities/stds-2002/cf.htm
Simplified
test procedure
Easy
test procedure
■ Test principle based on reverse hybridization line probe assay
(LiPA) on strip.
■ Can be processed manually or automatically using the Auto-LiPA.
■ INNO-LiPA CFTR19 and CFTR17+Tn Update tests can be run
simultaneously in one amplification and assay run due to identical
test procedures.
■ Only two amplicons are needed to detect and identify 36 mutations.
New
layout
Stripstrip
layout
INNO-LiPA CFTR17+ Tn
Update
INNO-LiPA CFTR19
Automation
Objective reading
®
™
Auto-LiPA
LiRAS for LiPA Cystic Fibrosis
■ Complete automation from hybridization to color development.
■ Automated reading of the strips.
■ Processing
of up
upto
to48
48tests
strips
upcoming
Processing of
perper
runrun
on on
thethe
Auto-LiPA
48.
48. of test manipulation: higher accuracy and
■ Auto-LiPA
Standardization
■ Complete interpretation
typing and interpretation
of reports.
and generation
of reports.
■ Scanned or manual entry.
■
trail
and filter management.
■ Audit
Scanner
calibration.
■ Standardization
reproducibility. of test manipulation: higher accuracy and
reproducibility.
trail and
filter management.
■ Audit
Test review
management.
Presentation
INNO-LiPA CFTR19
INNO-LiPA CFTR17 + Tn Update
®
LiRASTwin
for LiPA
CFTR
Set Cystic Fibrosis
LiRAS™ for LiPA Cystic Fibrosis
20 tests
20 tests
2 x 25 tests
Ask
more
information
about
new
products
Ask
forfor
more
information
about
our our
other
CFTR
products
art. no.
INNO-LiPA
Twin Set CFTR Italian Regional
80560
80558
80675
80604
80249
■ 21
Ready-to-use
controls
for INNO-LiPA
mutations and
wild-types
specific forCFTR.
the Italian population.
■ Same
36 mutant
probes, 36aswild-type
probes,
plus
5T, 7T, and 9T.
■
test procedure
INNO-LiPA
CFTR19
and
■ INNO-LiPA
Assures correct
handlingUpdate.
from hybridization to color development.
CFTR17+Tn
■ Assures the presence and reactivity of each probe on the strip.
CFTR testing by microarray technology
■ Assures the specificity of each probe on the strip.
■ Under development.
05130
Test format
©©Innogenetics
Innogenetics 2004
2008
■ Laboratory Instrument Management System (LIMS) compatible.
Regional panels
Commercial office: INNOGENETICS N.V.
Technologiepark 6 - B-9052 ZWIJNAARDE - BELGIUM
Tel. +32-(0)9-329 13 29 - Fax +32-(0)9-329 17 75
www.innogenetics.com
14898
■ Under development.