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Objectives
1. Be more familiar with the potential breeches in asepsis in an
A Review of Asepsis and
Safe Medication Practices
ASC.
2. Be able to discuss the 7 Principles of Surgical Asepsis
3. Describe safe medication practices in the ASC practice
setting.
Mary L. Gish, DNP, RN, NEA-BC
Nurse Consultant III
Center for Health Care Quality
Licensing and Certification
Asepsis
! 
Asepsis is the state of being free from disease-causing
contaminants (such as bacteria, viruses, fungi, and parasites)
or, preventing contact with microorganisms. The term asepsis
often refers to those practices used to promote or induce
asepsis in an operative field in surgery or medicine to prevent
infection.
Essential Components of Medical
Asepsis
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Handwashing
Utilizing gloves, gowns and masks as indicated
Cleaning equipment
Handling linens properly
Medical vs Surgical Asepsis
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Medical asepsis or clean asepsis is the state of being free
from disease causing microorganisms. Medical asepsis is
concerned with eliminating the spread of microorganisms
through facility practices.
Surgical or Sterile Asepsis includes procedures to eliminate
micro-organisms from an area and is practiced by the members
of the surgical team in operating rooms and treatment areas.
Hand Hygiene
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Sanitizer or Hand Wash
Before and after gloving (sterile or clean)
Medication Administration
Feeding residents (before and after)
Any invasive procedure (catheterization, blood glucose)
Contact with resident
Are sinks and hand sanitizers readily available?
Are gloves readily accessible?
Hand wash only for dietary,
GI symptoms
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Aseptic Technique
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Chain of Infection
Infectious Disease Cycle
Aseptic technique refers to a procedure that is performed under
sterile conditions. This includes medical and laboratory
techniques, such as with cultures.
Pathogen The largest example of aseptic techniques is in hospital
operating suites.
Host Reservoir Portal of Exit New Host (Disease) Aseptic technique is the effort taken to keep patients as free
from micro-organisms as possible
Portal of Entry Infection Process
Mode of Transmission
Agents
•  Bacteria
!  Aerobic
!  Anaerobic
•  Viruses
!  HBV
!  Influenza
•  Fungi
!  Responsible for some of the most common infections
•  Protozoa
Entrance of Microorganisms
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! 
Skin is the first line of defense
Depends on nature of the pathogen
•  Influenza (Respiratory Droplet)
•  MRSA (Contact)
•  HIV/HBV (Blood and Body Fluids)
Mode of Transmission 1. Fomite
•  Inanimate objects
•  Stethoscope, thermometer, scissors
2. Vector
•  Living carrier
•  Human hands
Infection Process
! 
Host
•  A micro-organism must accept the host
•  Infection develops as the strength and numbers grow in host
•  Immunizations have proved effective in providing additional
protection against infectious disease
2
Medical Asepsis =
Standard Precautions
1. 
2. 
3. 
4. 
5. 
6. 
7. 
Combines Universal Precautions and BSI
Applies to all patient encounters
Emphasizes hand hygiene
Use of gloves, gown masks and eye protection depending on the
anticipated exposure
Safe injection practices
Equipment or items in the resident environment likely to have been
contaminated with infectious body fluids must be handled in a
manner to prevent transmission
Education and training are the foundation
Transmission Based Precautions
! 
3 Categories
•  Contact Precautions - excessive wound drainage, fecal
incontinence, or other
•  Droplet Precautions – influenza, group A Strep
•  Airborne Precautions - TB
Surgical Asepsis
! 
Requires the absence of all microorganisms, pathogens and
spores from an object
Standard Precautions
Combines Universal Precautions and BSI
Applies to all residents
Emphasizes hand hygiene
Use of gloves, gown masks and eye protection depending on the
anticipated exposure
Safe injection practices
Equipment or items in the resident environment likely to have been
contaminated with infectious body fluids must be handled in a
manner to prevent transmission
Education and training are the foundation
1. 
2. 
3. 
4. 
5. 
6. 
7. 
Hand Hygiene
• 
• 
• 
• 
• 
• 
• 
• 
• 
Sanitizer or Hand Wash
Before and after gloving (sterile or clean)
Medication Administration
Feeding residents (before and after)
Any invasive procedure (catheterization, blood glucose)
Contact with resident
Are sinks and hand sanitizers readily available?
Are gloves readily accessible?
Hand wash only for dietary,
GI symptoms
Surgical Asepsis
Principles of Sterile technique
•  A sterile object remains sterile until touched by another
sterile object
•  Only sterile objects may be placed on a sterile field
•  A sterile object or field out of vision is contaminated
•  A sterile object or filed becomes contaminated by prolonged
exposure to air
3
Surgical Asepsis
Principles of Sterile technique
•  When a sterile surface comes in contact with a wet,
contaminated surface, the sterile object of field becomes
contaminated
•  Fluids flow in the direction of gravity
•  The edge of the sterile field or container is consider
contaminated
Surgical Asepsis
! 
Opening Sterile packages
•  Labels indicate the date that sterilization expires
•  Compromised packaging are no longer considered sterile
•  Providers follow strict handwashing protocol
Surgical Asepsis Principles
Surgical Asepsis Principles
Principle # 1: Scrubbed persons function within a sterile field
Principle #2 Sterile drapes are used to crate a sterile field
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Surgical team consists of sterile and non-sterile members
Sterile=Scrubbed
Non sterile remain in periphery
All wear scrub attire
Scrubbed persons wear sterile gown, mask, gloves, shields
Sterile areas
•  Gown front from chest to sterile field level
•  Sleeves 2” above elbow to cuff
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Establish an aseptic barrier minimizing the passage of
microorganisms
Places on patient, furniture, and equipment to be included in the
sterile field
Only incisional area is exposed
Only scrubbed personnel handle drapes
Held higher than the OR table, from incision out
Not moved or rearranged
Top surface is only area sterile
Surgical Asepsis Principles
Surgical Asepsis Principles
Principle #3: All items used on a field must be sterile
Principle #4: All items introduced to the sterile field should be
opened, dispensed, and transferred by methods that maintain
sterility and integrity
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Sterile and non sterile never mix
Sterility is determined by events not by time
Items are inspected for package integrity and indicators
Otherwise considered contaminated
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Non-sterile personnel (i.e. Circ Nurse) uses good judgment
when dispensing items
Tossing may compromise field by tearing, displacing
The 5 minute rule does not apply
4
Surgical Asepsis Principles
Principle #4
Principle #4 continued
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Open the top wrapper away first, then open the flaps to each
side
The last wrapper flap is pulled toward the nonsterile person
opened the package
Once opened contents are sterile up to 1 inch outer edge
Margin of safety
Double wrapped relies on institution policy
Surgical Asepsis Principles
Surgical Asepsis Principles
Principle #5: A sterile field should be maintained and monitored
constantly
Principle #6: Personnel should move around the field in a manner
that maintains sterility
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Must be monitored by all members of OR team
Sterility cannot be guaranteed but team must be vigilant
Breeches require immediate action to correct
Prepared as close to the OR time as possible
Time and exposure are risk factors
Risks: personnel, airborne contaminants, insects and liquids
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Establish safe distance or margin of safety between sterile and
nonsterile areas
Non-sterile personnel remain in non sterile areas
Always face the field
Never walk between two sterile fields
Never reach over or risk contact of touching sterile field
Surgical Asepsis Principles
Surgical Asepsis Principles
Principle #6: Personnel should move around the field in a manner
that maintains sterility
Principle #7: Policies and Procedures for maintenance of sterile
field should written, reviewed annually and available in practice
setting
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Patient is the center with scrubbed personnel close without
movement away
Move from sterile to sterile only
Scrub personnel keep distance and pass face to fact or back to
back
Scrubbed keep same position throughout procedure
Arms and hands always in the field
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Included in initial orientation and ongoing education of staff
Training of aseptic technique and practices by skilled members
to new and inexperienced.
Mentors and preceptors
5
New Work Being Done
Safe Injection Practices
Pharmacy Guidelines
Never re-use needles
Never share lancet holders
Never share insulin pens
Never use finger stick devices for more than one person
Never use a blood glucose meter for more than one
person without cleaning and disinfecting it in
between uses
•  Never use insulin pens for more than one person
•  Never fail to change gloves and perform hand
hygiene between finger stick procedures
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ASHP(2013). Drug Distribution and Control: Preparation and Handling–Guidelines. ASHP
Guidelines on Compounding Sterile Preparations, retrieved 10/24/2014 from http://www.ashp.org/
doclibrary/bestpractices/prepgdlcsp.aspx
Pharmacy Guidelines
Medication Safety
What is a multi-dose vial?
! 
! 
Multiple dose vials my be used for more than on patients as long as they are not
accessed in a patient care area.
ASHP (2013). Drug Distribution and Control: Preparation and Handling–Guidelines. ASHP Guidelines on
Compounding Sterile Preparations, retrieved 10/24/2014 from http://www.ashp.org/doclibrary/bestpractices/prepgdlcsp.aspx
! 
A multi-dose vial is a vial of liquid parenteral medication
(injection or infusion) that contains more than one dose of
medication.
Multi-dose vials are labeled as such by the manufacturer and
typically contain an antimicrobial preservative to help prevent
the growth of bacteria.
The preservative has no effect on viruses
6
Medication Safety
Can multi-dose vials be used for more than one patient? How?
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Dedicated to a single
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Examples of the immediate patient treatment area include
patient rooms or bays, hallways and operating rooms.
If a multi-dose vial enters the immediate patient treatment
area, it should be dedicated to that patient only and discarded
after use.
! 
When should multi-dose vials be discarded?
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Whenever sterility is compromised or questionable.
! 
What are examples of the “immediate patient treatment area”?
More than one patient not kept or accessed in the immediate
patient treatment area.
Medication Safety
! 
Medication Safety
Medication Safety Video
If a multi-dose has been opened or accessed (28 days or
manufacturer’s date, soonest)
Unopened = manufacturer’s expiration date.
Tools Available for Use
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CMS Exhibit 351
Infection Prevention Checklist for Outpatient Settings: Minimum
Expectation for Safe Care
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References
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ASHP(2013). Drug Distribution and Control: Preparation and Handling–Guidelines.
ASHP Guidelines on Compounding Sterile Preparations, retrieved 10/24/2014 from
http://www.ashp.org/doclibrary/bestpractices/prepgdlcsp.aspx
http://oneandonlycampaign.org/content/audio-video
http://antt.org/ANTT_Site/resources.html
http://www.cdc.gov/injectionsafety/providers/provider_faqs_multivials.html
Leopold, J. (2014)Aseptic Technique: Principles and Practices. AORN Journal,
Volume 94 , Issue 2 , 213 – 214
http://www.ismp.org/selfassessments/default.asp
http://www.cms.gov/Regulations-and-Guidance/Guidance/Manuals/downloads/
som107_exhibit_351.pdf
AORN Journal 2011 94, 213-214DOI: (10.1016/j.aorn.2011.04.018)
Copyright © 2011 AORN, Inc
Nurse Consultant
Infection Prevention and Control
Mary Gish, DNP, RN, NEA-BC
Nurse Consultant III, Infection Control
916-552-8636
[email protected]
Thank You!
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